Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table
The first aim of this study was to determine the prevalence and number of thermophilic Campylobacter spp. (Campylobacter jejuni, Campylobacter coli and Campylobacter fetus) in raw vegetables (ulam) at pre-harvest and retail level, soil and animal manure in an organic and a traditional vegetable f...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English English |
| Published: |
2008
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/5340/1/FSTM_2008_5a.pdf |
| _version_ | 1825943843887906816 |
|---|---|
| author | Chai, Lay Ching |
| author_facet | Chai, Lay Ching |
| author_sort | Chai, Lay Ching |
| collection | UPM |
| description | The first aim of this study was to determine the prevalence and number of
thermophilic Campylobacter spp. (Campylobacter jejuni, Campylobacter coli and
Campylobacter fetus) in raw vegetables (ulam) at pre-harvest and retail level,
soil and animal manure in an organic and a traditional vegetable farm. The
biosafety of Campylobacter jejuni was assessed by phenotypic (antibiotic
resistance) and genotypic (presence of virulent and toxin genes) as well as
RAPD-PCR characteristics of the strains isolated from vegetables. A kitchen
simulation study was conducted to provide decontamination and crosscontamination
data and information for estimation of the risk of acquiring
campylobacteriosis from consumption of ulam using a step-wise risk
assessment. The prevalence of thermophilic Campylobacter spp. in 309 (number of
samples) raw vegetables purchased from two supermarkets and a wet
market was relatively high, 29% to 68%. Campylobacter jejuni (25.5% to 67.7%)
and C. coli (21.6% to 65.7%) were predominant species isolated; while C.
fetus was only detected in two samples (1.9%) from one of the supermarkets.
Only 18.3% of Campylobacter-MPN-PCR positive samples were recovered by
enumeration-plating method indicating that routine enumeration-plating
methods has very low recovery rate for Campylobacter spp. from vegetables.
The study was extended to investigate the level of contamination with
Campylobacter spp. in vegetables farms. A total of 172 samples of animal
manure (n=18), soil (n=60), irrigation water (n=45) and vegetables (n=49)
samples were collected from both an organic and a conventional vegetable
farm. The organic vegetable farm (20.5%) was found to have a higher
prevalence of Campylobacter spp. compared to the vegetable farm practicing
conventional farming (2%). The low contamination level in the conventional
farm was most probably due to the bed-burning practice and the use of
composted manure in the farm. Campylobacter coli was not detected in all the
samples from both farms. Soil (30.4%) and animal manure (57.1%) sampled from the organic vegetable farm were found to harbor Campylobacter spp.
and C. jejuni. However, none of the irrigation water samples examined from
both farms were positive for Campylobacter spp.
RAPD-PCR fingerprinting and antibiotic resistance profiling indicated that
multi-resistant Campylobacter spp. might be wide-spread in the study area.
Clustering of C. jejuni isolates based on RAPD-PCR profiles suggested that
some isolates from different sources and locations were genotypically
closely related. Clusters A2, A3, A5 and A6 comprised C. jejuni strains
isolated from raw vegetables in the supermarkets and a wet market. All
clusters including B1 and B3, which comprised strains only from
supermarkets, were actually consisted of isolates from different sources. The
isolates showed multi-resistance to as many as 10 antibiotics tested. All the
isolates were detected to carry the virulent genes, cadF, ceuE and flaA.
However, toxin genes detection indicated only 16.1% and 10.7% of the
isolates carry cdtB and cdtC toxin genes, respectively; while none of the
isolates carry cdtA gene.
The potential of raw salad vegetables as a vehicle in C. jejuni transmission
was demonstrated by a step-wise risk assessment. Based on the assumptions used in the step-wise risk assessment, the annual number of
cases of campylobacteriosis acquired from the consumption of ulam is
estimated to be 4992/100,000 of Malaysian population, assuming that 10% of
Campylobacter spp. infection translates into illness. However, the risk
estimate was predicted to reduce to 175/100,000 if an extra blanching step
was incorporated into the model. In conclusion, there is an immediate need
for further investigation to look into the wide-spread problem of
Campylobacter spp. in ready-to-eat foods, such as salad and ulam, in Malaysia. |
| first_indexed | 2024-03-06T07:06:37Z |
| format | Thesis |
| id | upm.eprints-5340 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:06:37Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | upm.eprints-53402013-05-27T07:22:07Z http://psasir.upm.edu.my/id/eprint/5340/ Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table Chai, Lay Ching The first aim of this study was to determine the prevalence and number of thermophilic Campylobacter spp. (Campylobacter jejuni, Campylobacter coli and Campylobacter fetus) in raw vegetables (ulam) at pre-harvest and retail level, soil and animal manure in an organic and a traditional vegetable farm. The biosafety of Campylobacter jejuni was assessed by phenotypic (antibiotic resistance) and genotypic (presence of virulent and toxin genes) as well as RAPD-PCR characteristics of the strains isolated from vegetables. A kitchen simulation study was conducted to provide decontamination and crosscontamination data and information for estimation of the risk of acquiring campylobacteriosis from consumption of ulam using a step-wise risk assessment. The prevalence of thermophilic Campylobacter spp. in 309 (number of samples) raw vegetables purchased from two supermarkets and a wet market was relatively high, 29% to 68%. Campylobacter jejuni (25.5% to 67.7%) and C. coli (21.6% to 65.7%) were predominant species isolated; while C. fetus was only detected in two samples (1.9%) from one of the supermarkets. Only 18.3% of Campylobacter-MPN-PCR positive samples were recovered by enumeration-plating method indicating that routine enumeration-plating methods has very low recovery rate for Campylobacter spp. from vegetables. The study was extended to investigate the level of contamination with Campylobacter spp. in vegetables farms. A total of 172 samples of animal manure (n=18), soil (n=60), irrigation water (n=45) and vegetables (n=49) samples were collected from both an organic and a conventional vegetable farm. The organic vegetable farm (20.5%) was found to have a higher prevalence of Campylobacter spp. compared to the vegetable farm practicing conventional farming (2%). The low contamination level in the conventional farm was most probably due to the bed-burning practice and the use of composted manure in the farm. Campylobacter coli was not detected in all the samples from both farms. Soil (30.4%) and animal manure (57.1%) sampled from the organic vegetable farm were found to harbor Campylobacter spp. and C. jejuni. However, none of the irrigation water samples examined from both farms were positive for Campylobacter spp. RAPD-PCR fingerprinting and antibiotic resistance profiling indicated that multi-resistant Campylobacter spp. might be wide-spread in the study area. Clustering of C. jejuni isolates based on RAPD-PCR profiles suggested that some isolates from different sources and locations were genotypically closely related. Clusters A2, A3, A5 and A6 comprised C. jejuni strains isolated from raw vegetables in the supermarkets and a wet market. All clusters including B1 and B3, which comprised strains only from supermarkets, were actually consisted of isolates from different sources. The isolates showed multi-resistance to as many as 10 antibiotics tested. All the isolates were detected to carry the virulent genes, cadF, ceuE and flaA. However, toxin genes detection indicated only 16.1% and 10.7% of the isolates carry cdtB and cdtC toxin genes, respectively; while none of the isolates carry cdtA gene. The potential of raw salad vegetables as a vehicle in C. jejuni transmission was demonstrated by a step-wise risk assessment. Based on the assumptions used in the step-wise risk assessment, the annual number of cases of campylobacteriosis acquired from the consumption of ulam is estimated to be 4992/100,000 of Malaysian population, assuming that 10% of Campylobacter spp. infection translates into illness. However, the risk estimate was predicted to reduce to 175/100,000 if an extra blanching step was incorporated into the model. In conclusion, there is an immediate need for further investigation to look into the wide-spread problem of Campylobacter spp. in ready-to-eat foods, such as salad and ulam, in Malaysia. 2008 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5340/1/FSTM_2008_5a.pdf Chai, Lay Ching (2008) Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table. PhD thesis, Universiti Putra Malaysia. Thermophilic bacteria Campylobacter English |
| spellingShingle | Thermophilic bacteria Campylobacter Chai, Lay Ching Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table |
| title | Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table |
| title_full | Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table |
| title_fullStr | Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table |
| title_full_unstemmed | Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table |
| title_short | Microbial Risk Assessment of Thermophilic Campylobacter SPP. in Raw Vegetables from Farm to Table |
| title_sort | microbial risk assessment of thermophilic campylobacter spp in raw vegetables from farm to table |
| topic | Thermophilic bacteria Campylobacter |
| url | http://psasir.upm.edu.my/id/eprint/5340/1/FSTM_2008_5a.pdf |
| work_keys_str_mv | AT chailayching microbialriskassessmentofthermophiliccampylobactersppinrawvegetablesfromfarmtotable |