Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis)
Thiamine or vitamin B1 comprises a pyrimidine moiety and a thiazole moiety. Thiamine pyrophosphate (TPP), the active form of thiamine, acts as a cofactor for various major enzymes for example transketolase (TK), α-ketoglutarate dehydrogenase (KGDH) and pyruvate dehydrogenase (PDH). In this study, th...
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| Format: | Article |
| Language: | English |
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Malaysian Palm Oil Board
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53470/1/Osmotic%2C%20oxidative%20and%20salinity%20stresses%20upregulate%20the%20expressions%20of%20Thiamine.pdf |
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| author | Zainal Abidin, Aisamuddin Ardi Wong, Sook Yee Abdul Rahman, Nur Syuhadah Che Idris, Zainor Hafisah Balia Yusof, Zetty Norhana |
| author_facet | Zainal Abidin, Aisamuddin Ardi Wong, Sook Yee Abdul Rahman, Nur Syuhadah Che Idris, Zainor Hafisah Balia Yusof, Zetty Norhana |
| author_sort | Zainal Abidin, Aisamuddin Ardi |
| collection | UPM |
| description | Thiamine or vitamin B1 comprises a pyrimidine moiety and a thiazole moiety. Thiamine pyrophosphate (TPP), the active form of thiamine, acts as a cofactor for various major enzymes for example transketolase (TK), α-ketoglutarate dehydrogenase (KGDH) and pyruvate dehydrogenase (PDH). In this study, the gene transcripts of first two enzymes in thiamine biosynthesis pathway, THIC and THI1/THI4 were identified and amplified from oil palm tissues. Primers were designed based on sequence comparison of the genes from Arabidopsis thaliana, Zea mays, Oryza sativa and Alnus glutinosa. The oil palm's response in terms of the expression profiles of these two thiamine biosynthesis genes to osmotic stress, salinity stress and oxidative stress were analysed in this project. This is due to the suggestion that TPP plays another important role, which is protecting plants against abiotic and biotic stresses. Both THIC and THI1/THI4 gene transcripts were successfully amplified and the expressions of the gene transcripts were detected using reverse transcriptase polymerase chain reaction (RT-PCR). The levels of expressions were measured and the results showed that the expression of THIC gene transcripts showed increase in gene expression in conjunction with increase concentration of stress inducer. |
| first_indexed | 2024-03-06T09:17:57Z |
| format | Article |
| id | upm.eprints-53470 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:17:57Z |
| publishDate | 2016 |
| publisher | Malaysian Palm Oil Board |
| record_format | dspace |
| spelling | upm.eprints-534702017-11-13T03:34:09Z http://psasir.upm.edu.my/id/eprint/53470/ Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis) Zainal Abidin, Aisamuddin Ardi Wong, Sook Yee Abdul Rahman, Nur Syuhadah Che Idris, Zainor Hafisah Balia Yusof, Zetty Norhana Thiamine or vitamin B1 comprises a pyrimidine moiety and a thiazole moiety. Thiamine pyrophosphate (TPP), the active form of thiamine, acts as a cofactor for various major enzymes for example transketolase (TK), α-ketoglutarate dehydrogenase (KGDH) and pyruvate dehydrogenase (PDH). In this study, the gene transcripts of first two enzymes in thiamine biosynthesis pathway, THIC and THI1/THI4 were identified and amplified from oil palm tissues. Primers were designed based on sequence comparison of the genes from Arabidopsis thaliana, Zea mays, Oryza sativa and Alnus glutinosa. The oil palm's response in terms of the expression profiles of these two thiamine biosynthesis genes to osmotic stress, salinity stress and oxidative stress were analysed in this project. This is due to the suggestion that TPP plays another important role, which is protecting plants against abiotic and biotic stresses. Both THIC and THI1/THI4 gene transcripts were successfully amplified and the expressions of the gene transcripts were detected using reverse transcriptase polymerase chain reaction (RT-PCR). The levels of expressions were measured and the results showed that the expression of THIC gene transcripts showed increase in gene expression in conjunction with increase concentration of stress inducer. Malaysian Palm Oil Board 2016-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53470/1/Osmotic%2C%20oxidative%20and%20salinity%20stresses%20upregulate%20the%20expressions%20of%20Thiamine.pdf Zainal Abidin, Aisamuddin Ardi and Wong, Sook Yee and Abdul Rahman, Nur Syuhadah and Che Idris, Zainor Hafisah and Balia Yusof, Zetty Norhana (2016) Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis). Journal of Oil Palm Research, 28 (3). pp. 308-319. ISSN 1511-2780 http://jopr.mpob.gov.my/osmotic-oxidative-and-salinity-stresses-upregulate-the-expressions-of-thiamine-vitamin-b1-biosynthesis-genes-thic-and-thi1thi4-in-oil-palm-elaeis-guineensis/ |
| spellingShingle | Zainal Abidin, Aisamuddin Ardi Wong, Sook Yee Abdul Rahman, Nur Syuhadah Che Idris, Zainor Hafisah Balia Yusof, Zetty Norhana Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis) |
| title | Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis) |
| title_full | Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis) |
| title_fullStr | Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis) |
| title_full_unstemmed | Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis) |
| title_short | Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis) |
| title_sort | osmotic oxidative and salinity stresses upregulate the expressions of thiamine vitamin b1 biosynthesis genes thic and thi1 thi4 in oil palm elaeis guineensis |
| url | http://psasir.upm.edu.my/id/eprint/53470/1/Osmotic%2C%20oxidative%20and%20salinity%20stresses%20upregulate%20the%20expressions%20of%20Thiamine.pdf |
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