PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae
Rice blast caused by Pyricularia oryzae is the most devastative disease especially under aerobic cultivation systems. The bio-efficacy of plant growth-promoting microorganisms: Pseudomonas aeruginosa (UPMP1), Corynebacterium agropyri (UPMP7), Enterobacter gergoviae (UPMP9) and Bacillus amyloliquefac...
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| Format: | Article |
| Language: | English |
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Springer
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53552/1/PGPM-induced%20defense-related%20enzymes%20in%20aerobic%20rice%20against%20.pdf |
| _version_ | 1825930873648709632 |
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| author | Ng, Lee Chuen Meon, Sariah Sariam, O. Othman, Radziah Zainal Abidin, M. A. |
| author_facet | Ng, Lee Chuen Meon, Sariah Sariam, O. Othman, Radziah Zainal Abidin, M. A. |
| author_sort | Ng, Lee Chuen |
| collection | UPM |
| description | Rice blast caused by Pyricularia oryzae is the most devastative disease especially under aerobic cultivation systems. The bio-efficacy of plant growth-promoting microorganisms: Pseudomonas aeruginosa (UPMP1), Corynebacterium agropyri (UPMP7), Enterobacter gergoviae (UPMP9) and Bacillus amyloliquefaciens (UPMS3), Trichoderma harzianum (UPMT1) and Trichoderma virens (UPMT2) in induction of defense-related enzymes against Pyricularia oryzae was evaluated in rice cultivated under aerobic conditions. Under dual culture plate testing, all PGPMs indicated antagonism against P. oryzae with percentage inhibition radial growth (PIRG) which ranged from 51.69–81.97 %. The bio-efficacy of the respective PGPM in induction of defense-related enzymes in rice seedlings was evaluated based on individual inoculation before challenged inoculation with P. oryzae under greenhouse conditions. Inoculation of all PGPMs significantly reduced rice leaf blast severity at day eight after P. oryzae inoculation. The reduction in rice leaf blast disease severity was associated to the increase of peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities in rice seedlings when pre-inoculated with PGPMs. The highest leaf blast disease reduction (59.17 %) occurred with rice seedlings pre-inoculated with C. agropyri (UPMP9), followed by P. aeruginosa (UPMP1) (40.65 %), T. harzianum (UPMT1) (42.23 %), T. virens (UPMT2) (20.85 %), E. gergoviae (UPMP9) (17.84 %) and B. amyloliquefaciens (UPMS3). The high efficiency of PGPM in leaf blast disease suppression was associated with significant increase in total microbial activity (FDA hydrolysis) in rhizosphera soil (4.80–7.86 μg/g/0.5 h) compared to the control (2.25 μg/g/0.5 h). Thus, the application of PGPM is a potential alternative approach in rice leaf blast disease management of aerobic rice. |
| first_indexed | 2024-03-06T09:18:11Z |
| format | Article |
| id | upm.eprints-53552 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:18:11Z |
| publishDate | 2016 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-535522017-11-07T08:22:31Z http://psasir.upm.edu.my/id/eprint/53552/ PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae Ng, Lee Chuen Meon, Sariah Sariam, O. Othman, Radziah Zainal Abidin, M. A. Rice blast caused by Pyricularia oryzae is the most devastative disease especially under aerobic cultivation systems. The bio-efficacy of plant growth-promoting microorganisms: Pseudomonas aeruginosa (UPMP1), Corynebacterium agropyri (UPMP7), Enterobacter gergoviae (UPMP9) and Bacillus amyloliquefaciens (UPMS3), Trichoderma harzianum (UPMT1) and Trichoderma virens (UPMT2) in induction of defense-related enzymes against Pyricularia oryzae was evaluated in rice cultivated under aerobic conditions. Under dual culture plate testing, all PGPMs indicated antagonism against P. oryzae with percentage inhibition radial growth (PIRG) which ranged from 51.69–81.97 %. The bio-efficacy of the respective PGPM in induction of defense-related enzymes in rice seedlings was evaluated based on individual inoculation before challenged inoculation with P. oryzae under greenhouse conditions. Inoculation of all PGPMs significantly reduced rice leaf blast severity at day eight after P. oryzae inoculation. The reduction in rice leaf blast disease severity was associated to the increase of peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities in rice seedlings when pre-inoculated with PGPMs. The highest leaf blast disease reduction (59.17 %) occurred with rice seedlings pre-inoculated with C. agropyri (UPMP9), followed by P. aeruginosa (UPMP1) (40.65 %), T. harzianum (UPMT1) (42.23 %), T. virens (UPMT2) (20.85 %), E. gergoviae (UPMP9) (17.84 %) and B. amyloliquefaciens (UPMS3). The high efficiency of PGPM in leaf blast disease suppression was associated with significant increase in total microbial activity (FDA hydrolysis) in rhizosphera soil (4.80–7.86 μg/g/0.5 h) compared to the control (2.25 μg/g/0.5 h). Thus, the application of PGPM is a potential alternative approach in rice leaf blast disease management of aerobic rice. Springer 2016-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53552/1/PGPM-induced%20defense-related%20enzymes%20in%20aerobic%20rice%20against%20.pdf Ng, Lee Chuen and Meon, Sariah and Sariam, O. and Othman, Radziah and Zainal Abidin, M. A. (2016) PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae. European Journal of Plant Pathology, 145 (1). pp. 167-175. ISSN 0929-1873; ESSN: 1573-8469 https://link.springer.com/article/10.1007/s10658-015-0826-1 10.1007/s10658-015-0826-1 |
| spellingShingle | Ng, Lee Chuen Meon, Sariah Sariam, O. Othman, Radziah Zainal Abidin, M. A. PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae |
| title | PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae |
| title_full | PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae |
| title_fullStr | PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae |
| title_full_unstemmed | PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae |
| title_short | PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia aryzae |
| title_sort | pgpm induced defense related enzymes in aerobic rice against rice leaf blast caused by pyricularia aryzae |
| url | http://psasir.upm.edu.my/id/eprint/53552/1/PGPM-induced%20defense-related%20enzymes%20in%20aerobic%20rice%20against%20.pdf |
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