Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines
Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction. Methods: The cytotoxic effect of n-butanol fraction, crude ethano...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/53559/1/Phenolics-saponins%20rich%20fraction%20of%20defatted%20kenaf%20seed%20meal%20exhibits%20cytotoxicity%20towards%20cancer%20cell%20lines.pdf |
| _version_ | 1825930875031781376 |
|---|---|
| author | Saiful Yazan, Latifah Abd Rahman, Napsiah Kim Wei, Chan Wan Abd Ghani, Wan Nor Hafiza Tor, Yin Sim Foo, Jhi Biau |
| author_facet | Saiful Yazan, Latifah Abd Rahman, Napsiah Kim Wei, Chan Wan Abd Ghani, Wan Nor Hafiza Tor, Yin Sim Foo, Jhi Biau |
| author_sort | Saiful Yazan, Latifah |
| collection | UPM |
| description | Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction. Methods: The cytotoxic effect of n-butanol fraction, crude ethanolic extract and aqueous fraction on breast cancer (MCF-7 and MDA-MB-231), colon cancer (HT29), lung cancer (A549), cervical cancer (HeLa) and normal mouse fibroblast (3T3) cell lines was determined using MTT assay. The morphological changes of the treated cells were observed under an inverted light microscope. Results: n-Butanol fraction was the most cytotoxic towards HT29 and MCF-7 cells in a dose-dependent manner compared to crude ethanolic extract and aqueous fraction (P < 0.05). The IC50 of n-butanol fraction for HT29 and MCF-7 was (780.00 ± 28.28) and (895.00 ± 7.07) μg/mL, respectively. Cell shrinkage, membrane blebbing and formation of apoptotic bodies were noted following treatment of HT29 cells with n-butanol fraction. Conclusions: In conclusion, n-butanol fraction was more cytotoxic than crude ethanolic extract and aqueous fraction towards the selected cancerous cell lines and induced apoptosis in HT29 cells. |
| first_indexed | 2024-03-06T09:18:12Z |
| format | Article |
| id | upm.eprints-53559 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:18:12Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-535592017-11-08T02:20:03Z http://psasir.upm.edu.my/id/eprint/53559/ Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines Saiful Yazan, Latifah Abd Rahman, Napsiah Kim Wei, Chan Wan Abd Ghani, Wan Nor Hafiza Tor, Yin Sim Foo, Jhi Biau Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction. Methods: The cytotoxic effect of n-butanol fraction, crude ethanolic extract and aqueous fraction on breast cancer (MCF-7 and MDA-MB-231), colon cancer (HT29), lung cancer (A549), cervical cancer (HeLa) and normal mouse fibroblast (3T3) cell lines was determined using MTT assay. The morphological changes of the treated cells were observed under an inverted light microscope. Results: n-Butanol fraction was the most cytotoxic towards HT29 and MCF-7 cells in a dose-dependent manner compared to crude ethanolic extract and aqueous fraction (P < 0.05). The IC50 of n-butanol fraction for HT29 and MCF-7 was (780.00 ± 28.28) and (895.00 ± 7.07) μg/mL, respectively. Cell shrinkage, membrane blebbing and formation of apoptotic bodies were noted following treatment of HT29 cells with n-butanol fraction. Conclusions: In conclusion, n-butanol fraction was more cytotoxic than crude ethanolic extract and aqueous fraction towards the selected cancerous cell lines and induced apoptosis in HT29 cells. Elsevier 2016-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53559/1/Phenolics-saponins%20rich%20fraction%20of%20defatted%20kenaf%20seed%20meal%20exhibits%20cytotoxicity%20towards%20cancer%20cell%20lines.pdf Saiful Yazan, Latifah and Abd Rahman, Napsiah and Kim Wei, Chan and Wan Abd Ghani, Wan Nor Hafiza and Tor, Yin Sim and Foo, Jhi Biau (2016) Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines. Asian Pacific Journal of Tropical Biomedicine, 6 (5). pp. 404-409. ISSN 2221-1691; ESSN: 2588-9222 http://www.sciencedirect.com/science/article/pii/S2221169115308431 10.1016/j.apjtb.2016.03.007 |
| spellingShingle | Saiful Yazan, Latifah Abd Rahman, Napsiah Kim Wei, Chan Wan Abd Ghani, Wan Nor Hafiza Tor, Yin Sim Foo, Jhi Biau Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines |
| title | Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines |
| title_full | Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines |
| title_fullStr | Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines |
| title_full_unstemmed | Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines |
| title_short | Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines |
| title_sort | phenolics saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines |
| url | http://psasir.upm.edu.my/id/eprint/53559/1/Phenolics-saponins%20rich%20fraction%20of%20defatted%20kenaf%20seed%20meal%20exhibits%20cytotoxicity%20towards%20cancer%20cell%20lines.pdf |
| work_keys_str_mv | AT saifulyazanlatifah phenolicssaponinsrichfractionofdefattedkenafseedmealexhibitscytotoxicitytowardscancercelllines AT abdrahmannapsiah phenolicssaponinsrichfractionofdefattedkenafseedmealexhibitscytotoxicitytowardscancercelllines AT kimweichan phenolicssaponinsrichfractionofdefattedkenafseedmealexhibitscytotoxicitytowardscancercelllines AT wanabdghaniwannorhafiza phenolicssaponinsrichfractionofdefattedkenafseedmealexhibitscytotoxicitytowardscancercelllines AT toryinsim phenolicssaponinsrichfractionofdefattedkenafseedmealexhibitscytotoxicitytowardscancercelllines AT foojhibiau phenolicssaponinsrichfractionofdefattedkenafseedmealexhibitscytotoxicitytowardscancercelllines |