Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms
In this study, we prepared a series of lutein nanodispersions via the solvent displacement method, by using surfactants with different stabilizing mechanisms. The surfactants used include Tween 80 (steric stabilization), sodium dodecyl sulfate (SDS; electrostatic stabilization), sodium caseinate (el...
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| Format: | Article |
| Language: | English |
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Royal Society of Chemistry
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53585/1/Physicochemical%2C%20morphological%20and%20cellular%20uptake%20properties%20of%20lutein.pdf |
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| author | Tan, Tai Boon Chu, Wern Cui Yussof, Nor Shariffa Abas, Faridah Mirhosseini, Hamed Cheah, Yoke Kqueen Nehdi, Imededdine Arbi Tan, Chin Ping |
| author_facet | Tan, Tai Boon Chu, Wern Cui Yussof, Nor Shariffa Abas, Faridah Mirhosseini, Hamed Cheah, Yoke Kqueen Nehdi, Imededdine Arbi Tan, Chin Ping |
| author_sort | Tan, Tai Boon |
| collection | UPM |
| description | In this study, we prepared a series of lutein nanodispersions via the solvent displacement method, by using surfactants with different stabilizing mechanisms. The surfactants used include Tween 80 (steric stabilization), sodium dodecyl sulfate (SDS; electrostatic stabilization), sodium caseinate (electrosteric stabilization) and SDS–Tween 80 (electrostatic–steric stabilization). We then characterized the resulting lutein nanodispersions in terms of their particle size, particle size distribution, zeta potential, lutein content, flow behavior, apparent viscosity, transmittance, color, morphological properties and their effects on cell viability and cellular uptake. The type of surfactant used significantly (p < 0.05) affected the physical properties of the nanodispersions, but the chemical properties (lutein content) remained unaffected. Transmission electron microscopy (TEM) images obtained from this study demonstrated that the solvent displacement method was capable of producing lutein nanodispersions containing spherical particles with sizes ranging from 66.20–125.25 nm, depending on the type of surfactant used. SDS and SDS–Tween 80 surfactants negatively affected the viability of the HT-29 cells used in this study. Thus, for the cellular uptake determination, only Tween 80 and sodium caseinate surfactants were used. The cellular uptake of the lutein nanodispersion stabilized by sodium caseinate was higher than that which was stabilized by Tween 80. All things considered, the type of surfactant with different stabilizing mechanisms did produce lutein nanodispersions with different characteristics. These findings would aid in future selection of surfactants in order to produce nanodispersions with desirable properties. |
| first_indexed | 2024-03-06T09:18:16Z |
| format | Article |
| id | upm.eprints-53585 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:18:16Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | upm.eprints-535852017-11-09T04:48:21Z http://psasir.upm.edu.my/id/eprint/53585/ Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms Tan, Tai Boon Chu, Wern Cui Yussof, Nor Shariffa Abas, Faridah Mirhosseini, Hamed Cheah, Yoke Kqueen Nehdi, Imededdine Arbi Tan, Chin Ping In this study, we prepared a series of lutein nanodispersions via the solvent displacement method, by using surfactants with different stabilizing mechanisms. The surfactants used include Tween 80 (steric stabilization), sodium dodecyl sulfate (SDS; electrostatic stabilization), sodium caseinate (electrosteric stabilization) and SDS–Tween 80 (electrostatic–steric stabilization). We then characterized the resulting lutein nanodispersions in terms of their particle size, particle size distribution, zeta potential, lutein content, flow behavior, apparent viscosity, transmittance, color, morphological properties and their effects on cell viability and cellular uptake. The type of surfactant used significantly (p < 0.05) affected the physical properties of the nanodispersions, but the chemical properties (lutein content) remained unaffected. Transmission electron microscopy (TEM) images obtained from this study demonstrated that the solvent displacement method was capable of producing lutein nanodispersions containing spherical particles with sizes ranging from 66.20–125.25 nm, depending on the type of surfactant used. SDS and SDS–Tween 80 surfactants negatively affected the viability of the HT-29 cells used in this study. Thus, for the cellular uptake determination, only Tween 80 and sodium caseinate surfactants were used. The cellular uptake of the lutein nanodispersion stabilized by sodium caseinate was higher than that which was stabilized by Tween 80. All things considered, the type of surfactant with different stabilizing mechanisms did produce lutein nanodispersions with different characteristics. These findings would aid in future selection of surfactants in order to produce nanodispersions with desirable properties. Royal Society of Chemistry 2016-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53585/1/Physicochemical%2C%20morphological%20and%20cellular%20uptake%20properties%20of%20lutein.pdf Tan, Tai Boon and Chu, Wern Cui and Yussof, Nor Shariffa and Abas, Faridah and Mirhosseini, Hamed and Cheah, Yoke Kqueen and Nehdi, Imededdine Arbi and Tan, Chin Ping (2016) Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms. Food & Function, 7 (4). pp. 2043-2051. ISSN 2042-6496; ESSN: 2042-650X http://pubs.rsc.org/-/content/articlelanding/2016/fo/c5fo01621e/unauth#!divAbstract 10.1039/c5fo01621e |
| spellingShingle | Tan, Tai Boon Chu, Wern Cui Yussof, Nor Shariffa Abas, Faridah Mirhosseini, Hamed Cheah, Yoke Kqueen Nehdi, Imededdine Arbi Tan, Chin Ping Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms |
| title | Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms |
| title_full | Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms |
| title_fullStr | Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms |
| title_full_unstemmed | Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms |
| title_short | Physicochemical, morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms |
| title_sort | physicochemical morphological and cellular uptake properties of lutein nanodispersions prepared by using surfactants with different stabilizing mechanisms |
| url | http://psasir.upm.edu.my/id/eprint/53585/1/Physicochemical%2C%20morphological%20and%20cellular%20uptake%20properties%20of%20lutein.pdf |
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