Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease
Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alo...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53656/1/Preparation%2C%20characterization%2C%20and%20in%20ovo%20vaccination.pdf |
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| author | Firouzamandi, Masoumeh Moeini, Hassan Hosseini, Seyed Davood Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh El Zowalaty, Mohamed Ezzat Webster, Thomas Jay Ideris, Aini |
| author_facet | Firouzamandi, Masoumeh Moeini, Hassan Hosseini, Seyed Davood Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh El Zowalaty, Mohamed Ezzat Webster, Thomas Jay Ideris, Aini |
| author_sort | Firouzamandi, Masoumeh |
| collection | UPM |
| description | Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 µg pDNA/egg alone induced high levels of antibody titer (P<0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 µg pDNA + 64 µg D-SPM and 40 µg pDNA at 4 weeks postvaccination (P>0.05). Higher antibody titer was observed in the group immunized with 40 µg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 µg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery. |
| first_indexed | 2024-03-06T09:18:29Z |
| format | Article |
| id | upm.eprints-53656 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:18:29Z |
| publishDate | 2016 |
| publisher | Dove Medical Press |
| record_format | dspace |
| spelling | upm.eprints-536562017-11-23T10:26:42Z http://psasir.upm.edu.my/id/eprint/53656/ Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease Firouzamandi, Masoumeh Moeini, Hassan Hosseini, Seyed Davood Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh El Zowalaty, Mohamed Ezzat Webster, Thomas Jay Ideris, Aini Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 µg pDNA/egg alone induced high levels of antibody titer (P<0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 µg pDNA + 64 µg D-SPM and 40 µg pDNA at 4 weeks postvaccination (P>0.05). Higher antibody titer was observed in the group immunized with 40 µg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 µg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery. Dove Medical Press 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53656/1/Preparation%2C%20characterization%2C%20and%20in%20ovo%20vaccination.pdf Firouzamandi, Masoumeh and Moeini, Hassan and Hosseini, Seyed Davood and Bejo, Mohd Hair and Omar, Abdul Rahman and Mehrbod, Parvaneh and El Zowalaty, Mohamed Ezzat and Webster, Thomas Jay and Ideris, Aini (2016) Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease. International Journal of Nanomedicine, 2016 (11). pp. 259-267. ISSN 1176-9114; ESSN:1178-2013 https://www.dovepress.com/preparation-characterization-and-in-ovo-vaccination-of-dextran-spermin-peer-reviewed-article-IJN 10.2147/IJN.S92225 |
| spellingShingle | Firouzamandi, Masoumeh Moeini, Hassan Hosseini, Seyed Davood Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh El Zowalaty, Mohamed Ezzat Webster, Thomas Jay Ideris, Aini Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease |
| title | Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease |
| title_full | Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease |
| title_fullStr | Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease |
| title_full_unstemmed | Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease |
| title_short | Preparation, characterization and in ovo vaccination of dextran-spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease |
| title_sort | preparation characterization and in ovo vaccination of dextran spermine nanopeprints dna vaccine coexpressing the fusion and hemagglutinin genes against newcastle disease |
| url | http://psasir.upm.edu.my/id/eprint/53656/1/Preparation%2C%20characterization%2C%20and%20in%20ovo%20vaccination.pdf |
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