Regeneration potential of seedling explants of chilli (Capsicum annuum)
A study was conducted with hypocotyl, cotyledon and shoot tip of chilli as explants for regeneration on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Regeneration potential was determined by two ways. One is regeneration of shoot via callus formation...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5369/1/regeration_potential_of_seedling_explants.pdf |
| _version_ | 1796966949091016704 |
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| author | Md Ashrafuzzaman, Hossain, Mohammad Mosharraf Ismail, Mohd Razi Haque, M. Shahidul Shahidullah, S. M. Shahin-uz-zaman, |
| author_facet | Md Ashrafuzzaman, Hossain, Mohammad Mosharraf Ismail, Mohd Razi Haque, M. Shahidul Shahidullah, S. M. Shahin-uz-zaman, |
| author_sort | Md Ashrafuzzaman, |
| collection | UPM |
| description | A study was conducted with hypocotyl, cotyledon and shoot tip of chilli as explants for regeneration on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Regeneration potential was determined by two ways. One is regeneration of shoot via callus formation from hypocotyls and cotyledon explants; another was direct shoot regeneration from shoot tip explant. The highest callus was induced from hypocotyl in a combination of BAP (5.0 mgL-1) with NAA (0.1 mgL-1) and cotyledon in a combination of BAP (5.0 mgL-1) with IAA ((1.0 mgL-1). The callus induction as well as shoot initiation was higher in hypocotyls than cotyledon. Shoot tips regenerated into plantlets directly with sporadic small callus at the base. Shoot elongation was accelerated by using additional supplementation of GA3 and AgNO3. Regenerated shoots rooted best on the MS medium supplemented with 0.1 mg L-1 NAA + 0.05 mg L-1 IBA. |
| first_indexed | 2024-03-06T07:06:43Z |
| format | Article |
| id | upm.eprints-5369 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:06:43Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-53692017-11-14T09:05:05Z http://psasir.upm.edu.my/id/eprint/5369/ Regeneration potential of seedling explants of chilli (Capsicum annuum) Md Ashrafuzzaman, Hossain, Mohammad Mosharraf Ismail, Mohd Razi Haque, M. Shahidul Shahidullah, S. M. Shahin-uz-zaman, A study was conducted with hypocotyl, cotyledon and shoot tip of chilli as explants for regeneration on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Regeneration potential was determined by two ways. One is regeneration of shoot via callus formation from hypocotyls and cotyledon explants; another was direct shoot regeneration from shoot tip explant. The highest callus was induced from hypocotyl in a combination of BAP (5.0 mgL-1) with NAA (0.1 mgL-1) and cotyledon in a combination of BAP (5.0 mgL-1) with IAA ((1.0 mgL-1). The callus induction as well as shoot initiation was higher in hypocotyls than cotyledon. Shoot tips regenerated into plantlets directly with sporadic small callus at the base. Shoot elongation was accelerated by using additional supplementation of GA3 and AgNO3. Regenerated shoots rooted best on the MS medium supplemented with 0.1 mg L-1 NAA + 0.05 mg L-1 IBA. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5369/1/regeration_potential_of_seedling_explants.pdf Md Ashrafuzzaman, and Hossain, Mohammad Mosharraf and Ismail, Mohd Razi and Haque, M. Shahidul and Shahidullah, S. M. and Shahin-uz-zaman, (2009) Regeneration potential of seedling explants of chilli (Capsicum annuum). African Journal of Biotechnology, 8 (4). art. no. F27DFA18213. pp. 591-596. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/F27DFA18213 |
| spellingShingle | Md Ashrafuzzaman, Hossain, Mohammad Mosharraf Ismail, Mohd Razi Haque, M. Shahidul Shahidullah, S. M. Shahin-uz-zaman, Regeneration potential of seedling explants of chilli (Capsicum annuum) |
| title | Regeneration potential of seedling explants of chilli (Capsicum annuum) |
| title_full | Regeneration potential of seedling explants of chilli (Capsicum annuum) |
| title_fullStr | Regeneration potential of seedling explants of chilli (Capsicum annuum) |
| title_full_unstemmed | Regeneration potential of seedling explants of chilli (Capsicum annuum) |
| title_short | Regeneration potential of seedling explants of chilli (Capsicum annuum) |
| title_sort | regeneration potential of seedling explants of chilli capsicum annuum |
| url | http://psasir.upm.edu.my/id/eprint/5369/1/regeration_potential_of_seedling_explants.pdf |
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