Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy
The interaction of tetrabutylammonium bromide (TBABr)-based deep eutectic solvents (DESs) with calf thymus DNA (ct-DNA) was investigated by fluorescence and circular dichroism (CD) spectroscopy. The influences of hydrogen bond donors (HBDs), with respect to chain lengths and the presence of OH group...
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| Format: | Article |
| Language: | English |
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Malaysian Analytical Sciences Society
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53918/1/Studies%20of%20interaction%20between%20tetrabutylammonium%20bromide.pdf |
| _version_ | 1825930942026350592 |
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| author | Yusof, Rizana Ahmad, Haslina Abdul Rahman, Mohd Basyaruddin |
| author_facet | Yusof, Rizana Ahmad, Haslina Abdul Rahman, Mohd Basyaruddin |
| author_sort | Yusof, Rizana |
| collection | UPM |
| description | The interaction of tetrabutylammonium bromide (TBABr)-based deep eutectic solvents (DESs) with calf thymus DNA (ct-DNA) was investigated by fluorescence and circular dichroism (CD) spectroscopy. The influences of hydrogen bond donors (HBDs), with respect to chain lengths and the presence of OH group were determined through ethidium bromide (EB) fluorescence quenching. Based on the quenching process, it was concluded that hydrophobic interactions were responsible for the DES ability to quench EB from EB-bound DNA. The strong electrostatic attraction between TBA+ cation on DES and negative charge phosphate DNA was proved from fluorescence and CD spectroscopy. DNA was able to maintain its double helical structure at 25 °C with a concentration of 25% DES. |
| first_indexed | 2024-03-06T09:19:13Z |
| format | Article |
| id | upm.eprints-53918 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:19:13Z |
| publishDate | 2016 |
| publisher | Malaysian Analytical Sciences Society |
| record_format | dspace |
| spelling | upm.eprints-539182018-02-21T04:44:49Z http://psasir.upm.edu.my/id/eprint/53918/ Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy Yusof, Rizana Ahmad, Haslina Abdul Rahman, Mohd Basyaruddin The interaction of tetrabutylammonium bromide (TBABr)-based deep eutectic solvents (DESs) with calf thymus DNA (ct-DNA) was investigated by fluorescence and circular dichroism (CD) spectroscopy. The influences of hydrogen bond donors (HBDs), with respect to chain lengths and the presence of OH group were determined through ethidium bromide (EB) fluorescence quenching. Based on the quenching process, it was concluded that hydrophobic interactions were responsible for the DES ability to quench EB from EB-bound DNA. The strong electrostatic attraction between TBA+ cation on DES and negative charge phosphate DNA was proved from fluorescence and CD spectroscopy. DNA was able to maintain its double helical structure at 25 °C with a concentration of 25% DES. Malaysian Analytical Sciences Society 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/53918/1/Studies%20of%20interaction%20between%20tetrabutylammonium%20bromide.pdf Yusof, Rizana and Ahmad, Haslina and Abdul Rahman, Mohd Basyaruddin (2016) Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy. Malaysian Journal of Analytical Sciences, 20 (6). pp. 1233-240. ISSN 1394-2506 http://www.ukm.my/mjas/mjas2018/ Binding; Deep eutectic solvent; Tetrabutylammonium bromide; Fluorescence quenching; Circular dichroism spectroscopy 10.17576/mjas-2016-2006-01 |
| spellingShingle | Binding; Deep eutectic solvent; Tetrabutylammonium bromide; Fluorescence quenching; Circular dichroism spectroscopy Yusof, Rizana Ahmad, Haslina Abdul Rahman, Mohd Basyaruddin Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy |
| title | Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy |
| title_full | Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy |
| title_fullStr | Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy |
| title_full_unstemmed | Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy |
| title_short | Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy |
| title_sort | studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and dna using fluorescence quenching method and circular dichroism spectroscopy |
| topic | Binding; Deep eutectic solvent; Tetrabutylammonium bromide; Fluorescence quenching; Circular dichroism spectroscopy |
| url | http://psasir.upm.edu.my/id/eprint/53918/1/Studies%20of%20interaction%20between%20tetrabutylammonium%20bromide.pdf |
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