Effect of diet on the quality and quantity of venom produced by Apis cerana
The bee venom samples from honey bee (Apis cerana) foraged on seven plants dominating seven sites in Malaysia were collected. The protein content of the pollen loads of these test plants were tested according to the method of Kjeldahl (AOAC 1990). The samples were analysed to determine the effects o...
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Format: | Article |
Language: | English |
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EuroJournals Publishing
2016
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Online Access: | http://psasir.upm.edu.my/id/eprint/54003/1/Effect%20of%20diet%20on%20the%20quantity%20and%20quality%20of%20venom%20produced%20by%20Apis%20Cerana.pdf |
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author | Abusabbah, Mohammed Lau, Wei Hong Mahmoud, Mohammed E. E. M. Salih, Ashraf Omar, Dzolkhifli |
author_facet | Abusabbah, Mohammed Lau, Wei Hong Mahmoud, Mohammed E. E. M. Salih, Ashraf Omar, Dzolkhifli |
author_sort | Abusabbah, Mohammed |
collection | UPM |
description | The bee venom samples from honey bee (Apis cerana) foraged on seven plants dominating seven sites in Malaysia were collected. The protein content of the pollen loads of these test plants were tested according to the method of Kjeldahl (AOAC 1990). The samples were analysed to determine the effects of diet type on the quantity and quality of its constituents – melittin, phospholipase A2 and apamin using HPLC. The highest melittin (677.86μg/ml), phospholipase (477.96 μg/ml) and apamin (136.10 μg/ml) content were recorded in venom collected from honey bee colonies foraged on Durian plant which has protein content (31.7%) of its pollen loads. A positive correlation was recorded between the protein content with melittin (R2= 0.9372), phospholipase A2 (R2= 0.7208) and apamin (R2=0.4128), while a negative correlation was observed between the protein content and the weight of the venom mount produced (R2= -0.7549). A direct relationship was observed between the quality of the venom and the protein content of pollen loads, while the quantity of the venom was not in accordance with the venom quality. |
first_indexed | 2024-03-06T09:19:24Z |
format | Article |
id | upm.eprints-54003 |
institution | Universiti Putra Malaysia |
language | English |
last_indexed | 2024-03-06T09:19:24Z |
publishDate | 2016 |
publisher | EuroJournals Publishing |
record_format | dspace |
spelling | upm.eprints-540032018-02-22T09:29:38Z http://psasir.upm.edu.my/id/eprint/54003/ Effect of diet on the quality and quantity of venom produced by Apis cerana Abusabbah, Mohammed Lau, Wei Hong Mahmoud, Mohammed E. E. M. Salih, Ashraf Omar, Dzolkhifli The bee venom samples from honey bee (Apis cerana) foraged on seven plants dominating seven sites in Malaysia were collected. The protein content of the pollen loads of these test plants were tested according to the method of Kjeldahl (AOAC 1990). The samples were analysed to determine the effects of diet type on the quantity and quality of its constituents – melittin, phospholipase A2 and apamin using HPLC. The highest melittin (677.86μg/ml), phospholipase (477.96 μg/ml) and apamin (136.10 μg/ml) content were recorded in venom collected from honey bee colonies foraged on Durian plant which has protein content (31.7%) of its pollen loads. A positive correlation was recorded between the protein content with melittin (R2= 0.9372), phospholipase A2 (R2= 0.7208) and apamin (R2=0.4128), while a negative correlation was observed between the protein content and the weight of the venom mount produced (R2= -0.7549). A direct relationship was observed between the quality of the venom and the protein content of pollen loads, while the quantity of the venom was not in accordance with the venom quality. EuroJournals Publishing 2016-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54003/1/Effect%20of%20diet%20on%20the%20quantity%20and%20quality%20of%20venom%20produced%20by%20Apis%20Cerana.pdf Abusabbah, Mohammed and Lau, Wei Hong and Mahmoud, Mohammed E. E. and M. Salih, Ashraf and Omar, Dzolkhifli (2016) Effect of diet on the quality and quantity of venom produced by Apis cerana. European Journal of Scientific Research, 139 (4). pp. 387-394. ISSN 1450-216X; ESSN: 1450-202X http://www.europeanjournalofscientificresearch.com/issues/EJSR_139_4.html Apis cerana; Bee venom, Bee diet; HPLC |
spellingShingle | Apis cerana; Bee venom, Bee diet; HPLC Abusabbah, Mohammed Lau, Wei Hong Mahmoud, Mohammed E. E. M. Salih, Ashraf Omar, Dzolkhifli Effect of diet on the quality and quantity of venom produced by Apis cerana |
title | Effect of diet on the quality and quantity of venom produced by Apis cerana |
title_full | Effect of diet on the quality and quantity of venom produced by Apis cerana |
title_fullStr | Effect of diet on the quality and quantity of venom produced by Apis cerana |
title_full_unstemmed | Effect of diet on the quality and quantity of venom produced by Apis cerana |
title_short | Effect of diet on the quality and quantity of venom produced by Apis cerana |
title_sort | effect of diet on the quality and quantity of venom produced by apis cerana |
topic | Apis cerana; Bee venom, Bee diet; HPLC |
url | http://psasir.upm.edu.my/id/eprint/54003/1/Effect%20of%20diet%20on%20the%20quantity%20and%20quality%20of%20venom%20produced%20by%20Apis%20Cerana.pdf |
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