Effect of diet on the quality and quantity of venom produced by Apis cerana

The bee venom samples from honey bee (Apis cerana) foraged on seven plants dominating seven sites in Malaysia were collected. The protein content of the pollen loads of these test plants were tested according to the method of Kjeldahl (AOAC 1990). The samples were analysed to determine the effects o...

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Main Authors: Abusabbah, Mohammed, Lau, Wei Hong, Mahmoud, Mohammed E. E., M. Salih, Ashraf, Omar, Dzolkhifli
Format: Article
Language:English
Published: EuroJournals Publishing 2016
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/54003/1/Effect%20of%20diet%20on%20the%20quantity%20and%20quality%20of%20venom%20produced%20by%20Apis%20Cerana.pdf
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author Abusabbah, Mohammed
Lau, Wei Hong
Mahmoud, Mohammed E. E.
M. Salih, Ashraf
Omar, Dzolkhifli
author_facet Abusabbah, Mohammed
Lau, Wei Hong
Mahmoud, Mohammed E. E.
M. Salih, Ashraf
Omar, Dzolkhifli
author_sort Abusabbah, Mohammed
collection UPM
description The bee venom samples from honey bee (Apis cerana) foraged on seven plants dominating seven sites in Malaysia were collected. The protein content of the pollen loads of these test plants were tested according to the method of Kjeldahl (AOAC 1990). The samples were analysed to determine the effects of diet type on the quantity and quality of its constituents – melittin, phospholipase A2 and apamin using HPLC. The highest melittin (677.86μg/ml), phospholipase (477.96 μg/ml) and apamin (136.10 μg/ml) content were recorded in venom collected from honey bee colonies foraged on Durian plant which has protein content (31.7%) of its pollen loads. A positive correlation was recorded between the protein content with melittin (R2= 0.9372), phospholipase A2 (R2= 0.7208) and apamin (R2=0.4128), while a negative correlation was observed between the protein content and the weight of the venom mount produced (R2= -0.7549). A direct relationship was observed between the quality of the venom and the protein content of pollen loads, while the quantity of the venom was not in accordance with the venom quality.
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spelling upm.eprints-540032018-02-22T09:29:38Z http://psasir.upm.edu.my/id/eprint/54003/ Effect of diet on the quality and quantity of venom produced by Apis cerana Abusabbah, Mohammed Lau, Wei Hong Mahmoud, Mohammed E. E. M. Salih, Ashraf Omar, Dzolkhifli The bee venom samples from honey bee (Apis cerana) foraged on seven plants dominating seven sites in Malaysia were collected. The protein content of the pollen loads of these test plants were tested according to the method of Kjeldahl (AOAC 1990). The samples were analysed to determine the effects of diet type on the quantity and quality of its constituents – melittin, phospholipase A2 and apamin using HPLC. The highest melittin (677.86μg/ml), phospholipase (477.96 μg/ml) and apamin (136.10 μg/ml) content were recorded in venom collected from honey bee colonies foraged on Durian plant which has protein content (31.7%) of its pollen loads. A positive correlation was recorded between the protein content with melittin (R2= 0.9372), phospholipase A2 (R2= 0.7208) and apamin (R2=0.4128), while a negative correlation was observed between the protein content and the weight of the venom mount produced (R2= -0.7549). A direct relationship was observed between the quality of the venom and the protein content of pollen loads, while the quantity of the venom was not in accordance with the venom quality. EuroJournals Publishing 2016-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54003/1/Effect%20of%20diet%20on%20the%20quantity%20and%20quality%20of%20venom%20produced%20by%20Apis%20Cerana.pdf Abusabbah, Mohammed and Lau, Wei Hong and Mahmoud, Mohammed E. E. and M. Salih, Ashraf and Omar, Dzolkhifli (2016) Effect of diet on the quality and quantity of venom produced by Apis cerana. European Journal of Scientific Research, 139 (4). pp. 387-394. ISSN 1450-216X; ESSN: 1450-202X http://www.europeanjournalofscientificresearch.com/issues/EJSR_139_4.html Apis cerana; Bee venom, Bee diet; HPLC
spellingShingle Apis cerana; Bee venom, Bee diet; HPLC
Abusabbah, Mohammed
Lau, Wei Hong
Mahmoud, Mohammed E. E.
M. Salih, Ashraf
Omar, Dzolkhifli
Effect of diet on the quality and quantity of venom produced by Apis cerana
title Effect of diet on the quality and quantity of venom produced by Apis cerana
title_full Effect of diet on the quality and quantity of venom produced by Apis cerana
title_fullStr Effect of diet on the quality and quantity of venom produced by Apis cerana
title_full_unstemmed Effect of diet on the quality and quantity of venom produced by Apis cerana
title_short Effect of diet on the quality and quantity of venom produced by Apis cerana
title_sort effect of diet on the quality and quantity of venom produced by apis cerana
topic Apis cerana; Bee venom, Bee diet; HPLC
url http://psasir.upm.edu.my/id/eprint/54003/1/Effect%20of%20diet%20on%20the%20quantity%20and%20quality%20of%20venom%20produced%20by%20Apis%20Cerana.pdf
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