Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells
Patients with cancer often exhibit signs of anemia as the result of the disease. Thus, cancer chemotherapies often include erythropoietin (EPO) in the regime to improve the survival rate of these patients. The aim of the present study was to determine the effect of EPO on doxorubicin-treated breast...
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| Format: | Article |
| Language: | English |
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Spandidos Publications
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54047/1/Effect%20of%20recombinant%20human%20erythropoietin%20and%20doxorubicin.pdf |
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| author | Radwan, Esam M. Abdullah, Rasedee Al‑Qubaisi, Mothanna Sadiq El Zowalaty, Mohamed E. Naadja, Seif‑Eddine Mohamed Alitheen, Noorjahan Banu Omar, Abdul Rahman |
| author_facet | Radwan, Esam M. Abdullah, Rasedee Al‑Qubaisi, Mothanna Sadiq El Zowalaty, Mohamed E. Naadja, Seif‑Eddine Mohamed Alitheen, Noorjahan Banu Omar, Abdul Rahman |
| author_sort | Radwan, Esam M. |
| collection | UPM |
| description | Patients with cancer often exhibit signs of anemia as the result of the disease. Thus, cancer chemotherapies often include erythropoietin (EPO) in the regime to improve the survival rate of these patients. The aim of the present study was to determine the effect of EPO on doxorubicin-treated breast cancer cells. The cytotoxicity of doxorubicin alone or in combination with EPO against the MCF-7 and MDA-MB 231 human breast cancer cells were determined using an MTT cell viability assay, neutral red (NR) uptake assay and lactate dehydrogenase (LDH) assay. The estimated half maximal inhibitory concentration values for doxorubicin and the combination of doxorubicin with EPO were between 0.140 and 0.260 µg/ml for all cells treated for 72 h. Treatment with doxorubicin in combination with EPO led to no notable difference in cytotoxicity, compared with treatment with doxorubicin alone. The antiproliferative effect of doxorubicin at a concentration of 1 µg/ml on the MDA MB 231 cells was demonstrated by the decrease in viable cells from 3.6x10(5) at 24 h to 2.1x10(5) at 72 h of treatment. In order to confirm apoptosis in the doxorubicin-treated cells, the activities of caspases-3/7 and 9 were determined using a TBE assay. The results indicated that the activities of caspases-3/7 and 9 were significantly elevated in the doxorubicin-treated MDA-MB-231 cells by 571 and 645%, respectively, and in the MCF 7 cells by 471 and 345%, respectively, compared with the control cells. EPO did not modify the effect of doxorubicin on these cell lines. The results of the present study suggested that EPO was safe for use in combination with doxorubicin in the treatment of patients with breast cancer and concurrent anemia. |
| first_indexed | 2024-03-06T09:19:32Z |
| format | Article |
| id | upm.eprints-54047 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:19:32Z |
| publishDate | 2016 |
| publisher | Spandidos Publications |
| record_format | dspace |
| spelling | upm.eprints-540472018-02-26T09:29:41Z http://psasir.upm.edu.my/id/eprint/54047/ Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells Radwan, Esam M. Abdullah, Rasedee Al‑Qubaisi, Mothanna Sadiq El Zowalaty, Mohamed E. Naadja, Seif‑Eddine Mohamed Alitheen, Noorjahan Banu Omar, Abdul Rahman Patients with cancer often exhibit signs of anemia as the result of the disease. Thus, cancer chemotherapies often include erythropoietin (EPO) in the regime to improve the survival rate of these patients. The aim of the present study was to determine the effect of EPO on doxorubicin-treated breast cancer cells. The cytotoxicity of doxorubicin alone or in combination with EPO against the MCF-7 and MDA-MB 231 human breast cancer cells were determined using an MTT cell viability assay, neutral red (NR) uptake assay and lactate dehydrogenase (LDH) assay. The estimated half maximal inhibitory concentration values for doxorubicin and the combination of doxorubicin with EPO were between 0.140 and 0.260 µg/ml for all cells treated for 72 h. Treatment with doxorubicin in combination with EPO led to no notable difference in cytotoxicity, compared with treatment with doxorubicin alone. The antiproliferative effect of doxorubicin at a concentration of 1 µg/ml on the MDA MB 231 cells was demonstrated by the decrease in viable cells from 3.6x10(5) at 24 h to 2.1x10(5) at 72 h of treatment. In order to confirm apoptosis in the doxorubicin-treated cells, the activities of caspases-3/7 and 9 were determined using a TBE assay. The results indicated that the activities of caspases-3/7 and 9 were significantly elevated in the doxorubicin-treated MDA-MB-231 cells by 571 and 645%, respectively, and in the MCF 7 cells by 471 and 345%, respectively, compared with the control cells. EPO did not modify the effect of doxorubicin on these cell lines. The results of the present study suggested that EPO was safe for use in combination with doxorubicin in the treatment of patients with breast cancer and concurrent anemia. Spandidos Publications 2016-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54047/1/Effect%20of%20recombinant%20human%20erythropoietin%20and%20doxorubicin.pdf Radwan, Esam M. and Abdullah, Rasedee and Al‑Qubaisi, Mothanna Sadiq and El Zowalaty, Mohamed E. and Naadja, Seif‑Eddine and Mohamed Alitheen, Noorjahan Banu and Omar, Abdul Rahman (2016) Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells. Molecular Medicine Reports, 13 (5). pp. 3945-3952. ISSN 1791-2997; ESSN: 1791-3004 https://www.spandidos-publications.com/mmr/13/5/3945/abstract Erythropoietin; Doxorubicin; Proliferation; MCF 7; MDA MB231; Breast cancer 10.3892/mmr.2016.4989 |
| spellingShingle | Erythropoietin; Doxorubicin; Proliferation; MCF 7; MDA MB231; Breast cancer Radwan, Esam M. Abdullah, Rasedee Al‑Qubaisi, Mothanna Sadiq El Zowalaty, Mohamed E. Naadja, Seif‑Eddine Mohamed Alitheen, Noorjahan Banu Omar, Abdul Rahman Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells |
| title | Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells |
| title_full | Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells |
| title_fullStr | Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells |
| title_full_unstemmed | Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells |
| title_short | Effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of MCF-7 and MDA-mb231 breast cancer cells |
| title_sort | effect of recombinant human erythropoietin and doxorubicin in combination on the proliferation of mcf 7 and mda mb231 breast cancer cells |
| topic | Erythropoietin; Doxorubicin; Proliferation; MCF 7; MDA MB231; Breast cancer |
| url | http://psasir.upm.edu.my/id/eprint/54047/1/Effect%20of%20recombinant%20human%20erythropoietin%20and%20doxorubicin.pdf |
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