Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification
Effects of nutritional and physicochemical factors were investigated for cellulase production by the newly isolated thermophilic strain Bacillus licheniformis 2D55 (Accession No. KT799651). The optimum cellulase production in shake flask fermentation was attained at 60 °C, pH 3.5, 180 rpm, and in a...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54159/1/Enhanced%20cellulase%20production%20by%20a%20novel%20thermophilic%20Bacillus%20licheniformis%202D55.pdf |
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| author | Kazeem, Muinat Olanike Md Shah, Umi Kalsom Baharuddin, Azhari Shamsu Abdul Rahman, Nor' Aini |
| author_facet | Kazeem, Muinat Olanike Md Shah, Umi Kalsom Baharuddin, Azhari Shamsu Abdul Rahman, Nor' Aini |
| author_sort | Kazeem, Muinat Olanike |
| collection | UPM |
| description | Effects of nutritional and physicochemical factors were investigated for cellulase production by the newly isolated thermophilic strain Bacillus licheniformis 2D55 (Accession No. KT799651). The optimum cellulase production in shake flask fermentation was attained at 60 °C, pH 3.5, 180 rpm, and in a medium containing untreated sugarcane bagasse and pre-treated rice husk at 7% (w/v), urea, 1 g/L, peptone, 11.0 g/L, Mg(SO4)2, 0.40 g/L, CaCl2, 0.03 g/L, Tween 80, 0.2% (w/v), and 3% inoculum. The highest carboxymethyl cellulase (CMCase), filtre paperase (FPase), and β-glucosidase produced under the optimized conditions were 29.4 U/mL, 12.9 U/mL, and 0.06 U/mL, respectively, after 18 h of fermentation. Optimization of the parameters increased the CMCase, FPase, and β-glucosidase activities by 77.4-fold, 44.5-fold, and 10-fold, respectively. The crude enzyme was highly active and stable over broad temperature (50 to 80 °C) and pH (3.5 to 10.0) ranges with optimum temperature at 65 °C and 80 ºC for CMCase and FPase, respectively. The optimum pH for CMCase and FPase was 7.5 and 6.0, respectively. Saccharification of sugar cane bagasse and rice husk by crude cellulase resulted in perspective yields of 0.348 and 0.301 g g-1 dry substrate of reducing sugars. These results suggest prospects of thermostable cellulase from B. licheniformis 2D55 in application for bio-sugar production and other industrial bioprocess applications involving high temperatures. |
| first_indexed | 2024-03-06T09:19:51Z |
| format | Article |
| id | upm.eprints-54159 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:19:51Z |
| publishDate | 2016 |
| publisher | North Carolina State University |
| record_format | dspace |
| spelling | upm.eprints-541592018-03-01T07:58:07Z http://psasir.upm.edu.my/id/eprint/54159/ Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification Kazeem, Muinat Olanike Md Shah, Umi Kalsom Baharuddin, Azhari Shamsu Abdul Rahman, Nor' Aini Effects of nutritional and physicochemical factors were investigated for cellulase production by the newly isolated thermophilic strain Bacillus licheniformis 2D55 (Accession No. KT799651). The optimum cellulase production in shake flask fermentation was attained at 60 °C, pH 3.5, 180 rpm, and in a medium containing untreated sugarcane bagasse and pre-treated rice husk at 7% (w/v), urea, 1 g/L, peptone, 11.0 g/L, Mg(SO4)2, 0.40 g/L, CaCl2, 0.03 g/L, Tween 80, 0.2% (w/v), and 3% inoculum. The highest carboxymethyl cellulase (CMCase), filtre paperase (FPase), and β-glucosidase produced under the optimized conditions were 29.4 U/mL, 12.9 U/mL, and 0.06 U/mL, respectively, after 18 h of fermentation. Optimization of the parameters increased the CMCase, FPase, and β-glucosidase activities by 77.4-fold, 44.5-fold, and 10-fold, respectively. The crude enzyme was highly active and stable over broad temperature (50 to 80 °C) and pH (3.5 to 10.0) ranges with optimum temperature at 65 °C and 80 ºC for CMCase and FPase, respectively. The optimum pH for CMCase and FPase was 7.5 and 6.0, respectively. Saccharification of sugar cane bagasse and rice husk by crude cellulase resulted in perspective yields of 0.348 and 0.301 g g-1 dry substrate of reducing sugars. These results suggest prospects of thermostable cellulase from B. licheniformis 2D55 in application for bio-sugar production and other industrial bioprocess applications involving high temperatures. North Carolina State University 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54159/1/Enhanced%20cellulase%20production%20by%20a%20novel%20thermophilic%20Bacillus%20licheniformis%202D55.pdf Kazeem, Muinat Olanike and Md Shah, Umi Kalsom and Baharuddin, Azhari Shamsu and Abdul Rahman, Nor' Aini (2016) Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification. BioResources, 11 (2). pp. 5404-5423. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_2_5404_Kazeem_Enhanced_Cellulase_Production Bacillus licheniformis 2D55; Thermostable cellulase; Lignocellulosic saccharification; Optimization |
| spellingShingle | Bacillus licheniformis 2D55; Thermostable cellulase; Lignocellulosic saccharification; Optimization Kazeem, Muinat Olanike Md Shah, Umi Kalsom Baharuddin, Azhari Shamsu Abdul Rahman, Nor' Aini Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification |
| title | Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification |
| title_full | Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification |
| title_fullStr | Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification |
| title_full_unstemmed | Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification |
| title_short | Enhanced cellulase production by a novel thermophilic Bacillus licheniformis 2D55: characterization and application in lignocellulosic saccharification |
| title_sort | enhanced cellulase production by a novel thermophilic bacillus licheniformis 2d55 characterization and application in lignocellulosic saccharification |
| topic | Bacillus licheniformis 2D55; Thermostable cellulase; Lignocellulosic saccharification; Optimization |
| url | http://psasir.upm.edu.my/id/eprint/54159/1/Enhanced%20cellulase%20production%20by%20a%20novel%20thermophilic%20Bacillus%20licheniformis%202D55.pdf |
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