Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment
Background: Complement system is theoretically believed to halt the progression of tumor by the activity of C5a/CD88. Protein C5a is a potent pro.inflammatory mediator that activates the complement system by binding to its receptor. Objectives: The purpose of this study is to determine the expressi...
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| Format: | Article |
| Language: | English |
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Medknow Publications
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54248/1/Expression%20of%20complement%20C5a%20receptor.pdf |
| _version_ | 1796976043130617856 |
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| author | Kosni, Nurneqman Nashreq Ganti, Norhaifa Mohd Noor, Mohd Hezmee Abdul Razak, Intan Shameha Md Ajat, Mohd Mokrish Omar, Abdul Rahman |
| author_facet | Kosni, Nurneqman Nashreq Ganti, Norhaifa Mohd Noor, Mohd Hezmee Abdul Razak, Intan Shameha Md Ajat, Mohd Mokrish Omar, Abdul Rahman |
| author_sort | Kosni, Nurneqman Nashreq |
| collection | UPM |
| description | Background: Complement system is theoretically believed to halt the progression of tumor by the activity of C5a/CD88. Protein C5a is a potent pro.inflammatory mediator that activates the complement system by binding to its receptor.
Objectives: The purpose of this study is to determine the expression of the anaphylatoxin C5a receptor on 4T1 cell line and to study the viability of the cells after being treated with the C5a peptides. Materials and Methods: The cells 4T1 had undergone immunofluorescence staining, conventional polymerase chain reaction (PCR) and real-time PCR for the expression of determination part. Whereas Alamar Blue and MTT assays were conducted for the viability study of the cells. Results: The cells showed positive result in expressing the receptor of the C5a through immunostaining and PCR. The CT value recorded at initial dilution was 22.24. In cell viability assay, the cell was treated with C5a peptides, PMX205 and EP54. The purpose of this treatment was to see whether C5a had a direct effect on the cell itself using both assays. The result showed that PMX205, which is an antagonist, gave more effects towards the cell as compared with the treatment of EP54. Conclusion: This experiment shows the presence of C5a receptor on 4T1 cell line. We believe that the antagonist peptide is eligible to be used widely in cancer immunotherapy field; but in vivo studies need to be carried out first in the future, as it will determine how these drugs affect the tumor cell growth. |
| first_indexed | 2024-03-06T09:20:07Z |
| format | Article |
| id | upm.eprints-54248 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:20:07Z |
| publishDate | 2016 |
| publisher | Medknow Publications |
| record_format | dspace |
| spelling | upm.eprints-542482018-03-08T07:22:05Z http://psasir.upm.edu.my/id/eprint/54248/ Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment Kosni, Nurneqman Nashreq Ganti, Norhaifa Mohd Noor, Mohd Hezmee Abdul Razak, Intan Shameha Md Ajat, Mohd Mokrish Omar, Abdul Rahman Background: Complement system is theoretically believed to halt the progression of tumor by the activity of C5a/CD88. Protein C5a is a potent pro.inflammatory mediator that activates the complement system by binding to its receptor. Objectives: The purpose of this study is to determine the expression of the anaphylatoxin C5a receptor on 4T1 cell line and to study the viability of the cells after being treated with the C5a peptides. Materials and Methods: The cells 4T1 had undergone immunofluorescence staining, conventional polymerase chain reaction (PCR) and real-time PCR for the expression of determination part. Whereas Alamar Blue and MTT assays were conducted for the viability study of the cells. Results: The cells showed positive result in expressing the receptor of the C5a through immunostaining and PCR. The CT value recorded at initial dilution was 22.24. In cell viability assay, the cell was treated with C5a peptides, PMX205 and EP54. The purpose of this treatment was to see whether C5a had a direct effect on the cell itself using both assays. The result showed that PMX205, which is an antagonist, gave more effects towards the cell as compared with the treatment of EP54. Conclusion: This experiment shows the presence of C5a receptor on 4T1 cell line. We believe that the antagonist peptide is eligible to be used widely in cancer immunotherapy field; but in vivo studies need to be carried out first in the future, as it will determine how these drugs affect the tumor cell growth. Medknow Publications 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54248/1/Expression%20of%20complement%20C5a%20receptor.pdf Kosni, Nurneqman Nashreq and Ganti, Norhaifa and Mohd Noor, Mohd Hezmee and Abdul Razak, Intan Shameha and Md Ajat, Mohd Mokrish and Omar, Abdul Rahman (2016) Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment. Journal of Cancer Research and Therapeutics, 12 (2). pp. 590-596. ISSN 0973-1482; ESSN: 1998-4138 http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2016;volume=12;issue=2;spage=590;epage=596;aulast=Kosni C5a; C5aR; 4T1; C5a peptides; Tamoxifen 10.4103/0973-1482.146066 |
| spellingShingle | C5a; C5aR; 4T1; C5a peptides; Tamoxifen Kosni, Nurneqman Nashreq Ganti, Norhaifa Mohd Noor, Mohd Hezmee Abdul Razak, Intan Shameha Md Ajat, Mohd Mokrish Omar, Abdul Rahman Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment |
| title | Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment |
| title_full | Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment |
| title_fullStr | Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment |
| title_full_unstemmed | Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment |
| title_short | Expression of complement C5a receptor and the viability of 4T1 tumor cells following agonist–antagonist treatment |
| title_sort | expression of complement c5a receptor and the viability of 4t1 tumor cells following agonist antagonist treatment |
| topic | C5a; C5aR; 4T1; C5a peptides; Tamoxifen |
| url | http://psasir.upm.edu.my/id/eprint/54248/1/Expression%20of%20complement%20C5a%20receptor.pdf |
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