Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment
Mosquitoes are arthropods of huge medical and veterinary relevance, since they vector pathogens and parasites of public health importance, including malaria, dengue and Zika virus. Currently, nanotechnology is considered a potential eco-friendly approach in mosquito control research. We proposed a n...
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54260/1/Fabrication%20of%20nano-mosquitocides%20using%20chitosan%20from%20crab%20shells.pdf |
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| author | Murugan, Kadarkarai Jaganathan, Anitha Dinesh, Devakumar Suresh, Udaiyan Rajaganesh, Rajapandian Chandramohan, Balamurugan Subramaniam, Jayapal Paulpandi, Manickam Vadivalagan, Chitravel Amuthavalli, Pandiyan Wang, Lan Hwang, Jiang Shiou Wei, Hui Alsalhi, Mohamad Saleh Devanesan, Sandhanasamy Kumar, Suresh Pugazhendy, Kannaiyan Higuchi, Akon Nicoletti, Marcello Benelli, Giovanni |
| author_facet | Murugan, Kadarkarai Jaganathan, Anitha Dinesh, Devakumar Suresh, Udaiyan Rajaganesh, Rajapandian Chandramohan, Balamurugan Subramaniam, Jayapal Paulpandi, Manickam Vadivalagan, Chitravel Amuthavalli, Pandiyan Wang, Lan Hwang, Jiang Shiou Wei, Hui Alsalhi, Mohamad Saleh Devanesan, Sandhanasamy Kumar, Suresh Pugazhendy, Kannaiyan Higuchi, Akon Nicoletti, Marcello Benelli, Giovanni |
| author_sort | Murugan, Kadarkarai |
| collection | UPM |
| description | Mosquitoes are arthropods of huge medical and veterinary relevance, since they vector pathogens and parasites of public health importance, including malaria, dengue and Zika virus. Currently, nanotechnology is considered a potential eco-friendly approach in mosquito control research. We proposed a novel method of biofabrication of silver nanoparticles (AgNP) using chitosan (Ch) from crab shells. Ch-AgNP nanocomposite was characterized by UV–vis spectroscopy, FTIR, SEM, EDX and XRD. Ch-AgNP were tested against larvae and pupae of the malaria vector Anopheles stephensi obtaining LC50 ranging from 3.18 ppm (I) to 6.54 ppm (pupae). The antibacterial properties of Ch-AgNP were proved against Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi, while no growth inhibition was reported in assays conducted on Proteus vulgaris. Concerning non-target effects, in standard laboratory considtions the predation efficiency of Danio rerio zebrafishes was 68.8% and 61.6% against I and II instar larvae of A. stephensi, respectively. In a Ch-AgNP-contaminated environment, fish predation was boosted to 89.5% and 77.3%, respectively. Quantitative analysis of antioxidant enzymes SOD, CAT and LPO from hepatopancreas of fresh water crabs Paratelphusa hydrodromous exposed for 16 days to a Ch-AgNP-contaminated aquatic environment were conducted. Notably, deleterious effects of Ch-AgNP contaminating aquatic enviroment on the non-target crab P. hydrodromous were observed, particularly when doses higher than 8–10 ppm are tested. Overall, this research highlights the potential of Ch-AGNP for the development of newer control tools against young instar populations of malaria mosquitoes, also highlighting some risks concerned the employ of nanoparticles in aquatic environments. |
| first_indexed | 2024-03-06T09:20:09Z |
| format | Article |
| id | upm.eprints-54260 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:20:09Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-542602018-03-09T02:35:21Z http://psasir.upm.edu.my/id/eprint/54260/ Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment Murugan, Kadarkarai Jaganathan, Anitha Dinesh, Devakumar Suresh, Udaiyan Rajaganesh, Rajapandian Chandramohan, Balamurugan Subramaniam, Jayapal Paulpandi, Manickam Vadivalagan, Chitravel Amuthavalli, Pandiyan Wang, Lan Hwang, Jiang Shiou Wei, Hui Alsalhi, Mohamad Saleh Devanesan, Sandhanasamy Kumar, Suresh Pugazhendy, Kannaiyan Higuchi, Akon Nicoletti, Marcello Benelli, Giovanni Mosquitoes are arthropods of huge medical and veterinary relevance, since they vector pathogens and parasites of public health importance, including malaria, dengue and Zika virus. Currently, nanotechnology is considered a potential eco-friendly approach in mosquito control research. We proposed a novel method of biofabrication of silver nanoparticles (AgNP) using chitosan (Ch) from crab shells. Ch-AgNP nanocomposite was characterized by UV–vis spectroscopy, FTIR, SEM, EDX and XRD. Ch-AgNP were tested against larvae and pupae of the malaria vector Anopheles stephensi obtaining LC50 ranging from 3.18 ppm (I) to 6.54 ppm (pupae). The antibacterial properties of Ch-AgNP were proved against Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi, while no growth inhibition was reported in assays conducted on Proteus vulgaris. Concerning non-target effects, in standard laboratory considtions the predation efficiency of Danio rerio zebrafishes was 68.8% and 61.6% against I and II instar larvae of A. stephensi, respectively. In a Ch-AgNP-contaminated environment, fish predation was boosted to 89.5% and 77.3%, respectively. Quantitative analysis of antioxidant enzymes SOD, CAT and LPO from hepatopancreas of fresh water crabs Paratelphusa hydrodromous exposed for 16 days to a Ch-AgNP-contaminated aquatic environment were conducted. Notably, deleterious effects of Ch-AgNP contaminating aquatic enviroment on the non-target crab P. hydrodromous were observed, particularly when doses higher than 8–10 ppm are tested. Overall, this research highlights the potential of Ch-AGNP for the development of newer control tools against young instar populations of malaria mosquitoes, also highlighting some risks concerned the employ of nanoparticles in aquatic environments. Elsevier 2016-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54260/1/Fabrication%20of%20nano-mosquitocides%20using%20chitosan%20from%20crab%20shells.pdf Murugan, Kadarkarai and Jaganathan, Anitha and Dinesh, Devakumar and Suresh, Udaiyan and Rajaganesh, Rajapandian and Chandramohan, Balamurugan and Subramaniam, Jayapal and Paulpandi, Manickam and Vadivalagan, Chitravel and Amuthavalli, Pandiyan and Wang, Lan and Hwang, Jiang Shiou and Wei, Hui and Alsalhi, Mohamad Saleh and Devanesan, Sandhanasamy and Kumar, Suresh and Pugazhendy, Kannaiyan and Higuchi, Akon and Nicoletti, Marcello and Benelli, Giovanni (2016) Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment. Ecotoxicology and Environmental Safety, 132. 318 - 328. ISSN 0147-6513; ESSN: 1090-2414 https://www.sciencedirect.com/science/article/pii/S0147651316302275?via%3Dihub Anopheles stephensi; Biosafety; Culicidae; Danio rerio; Non-target effects; Plasmodium 10.1016/j.ecoenv.2016.06.021 |
| spellingShingle | Anopheles stephensi; Biosafety; Culicidae; Danio rerio; Non-target effects; Plasmodium Murugan, Kadarkarai Jaganathan, Anitha Dinesh, Devakumar Suresh, Udaiyan Rajaganesh, Rajapandian Chandramohan, Balamurugan Subramaniam, Jayapal Paulpandi, Manickam Vadivalagan, Chitravel Amuthavalli, Pandiyan Wang, Lan Hwang, Jiang Shiou Wei, Hui Alsalhi, Mohamad Saleh Devanesan, Sandhanasamy Kumar, Suresh Pugazhendy, Kannaiyan Higuchi, Akon Nicoletti, Marcello Benelli, Giovanni Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment |
| title | Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment |
| title_full | Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment |
| title_fullStr | Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment |
| title_full_unstemmed | Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment |
| title_short | Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment |
| title_sort | fabrication of nano mosquitocides using chitosan from crab shells impact on non target organisms in the aquatic environment |
| topic | Anopheles stephensi; Biosafety; Culicidae; Danio rerio; Non-target effects; Plasmodium |
| url | http://psasir.upm.edu.my/id/eprint/54260/1/Fabrication%20of%20nano-mosquitocides%20using%20chitosan%20from%20crab%20shells.pdf |
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