Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13
Bacillus subtilis UPMB13 was found to be an L-glutamic acid independent producer of extracellular polymeric substances (EPS) with bioflocculation properties. Optimum production of the bioflocculant was found to be at the early stage of cell propagation of 24–72 h of fermentation. At a limited nutrie...
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| Format: | Article |
| Language: | English |
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Springer Verlag
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54391/1/Glutamic%20acid%20independent%20production%20of%20bioflocculants%20by%20Bacillus%20subtilis%20UPMB13.pdf |
| _version_ | 1796976072669003776 |
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| author | Zulkeflee, Zufarzaana Shamsuddin, Zulkifli Aris, Ahmad Zaharin Yusoff, Mohd Kamil Komilis, Dimitrios Sánchez, Antoni |
| author_facet | Zulkeflee, Zufarzaana Shamsuddin, Zulkifli Aris, Ahmad Zaharin Yusoff, Mohd Kamil Komilis, Dimitrios Sánchez, Antoni |
| author_sort | Zulkeflee, Zufarzaana |
| collection | UPM |
| description | Bacillus subtilis UPMB13 was found to be an L-glutamic acid independent producer of extracellular polymeric substances (EPS) with bioflocculation properties. Optimum production of the bioflocculant was found to be at the early stage of cell propagation of 24–72 h of fermentation. At a limited nutrient input of 100 mL tryptic soy broth, the flocculating activities were found to be negatively correlated (p < 0.01) with growth as it continued to decline after 72 h, while cell growth proliferated further. Ample nutrient supply may prolong bioflocculant production with flocculating activities of 90 % and higher, while excess oxygen supply may promote rapid growth that can lead to poor flocculation due to the re-use of the bioflocculant as a substitute for food during starvation. Bioflocculant production occurred at best at 25-30 °C incubation temperature and at the initial pH medium of 7 to 8. The bioflocculant was proven to be extracellularly produced as the broth and the supernatant possessed the ability to flocculate the suspended kaolin particles. Bioflocculant productions by UPMB13 were hereditarily stable among succeeding progenies, hence, proving genetic competency. About 0.90 g of purified bioflocculant were collected from 1 L culture broth of UPMB13 under the optimized fermentation conditions. |
| first_indexed | 2024-03-06T09:20:32Z |
| format | Article |
| id | upm.eprints-54391 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:20:32Z |
| publishDate | 2016 |
| publisher | Springer Verlag |
| record_format | dspace |
| spelling | upm.eprints-543912018-03-15T03:08:05Z http://psasir.upm.edu.my/id/eprint/54391/ Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13 Zulkeflee, Zufarzaana Shamsuddin, Zulkifli Aris, Ahmad Zaharin Yusoff, Mohd Kamil Komilis, Dimitrios Sánchez, Antoni Bacillus subtilis UPMB13 was found to be an L-glutamic acid independent producer of extracellular polymeric substances (EPS) with bioflocculation properties. Optimum production of the bioflocculant was found to be at the early stage of cell propagation of 24–72 h of fermentation. At a limited nutrient input of 100 mL tryptic soy broth, the flocculating activities were found to be negatively correlated (p < 0.01) with growth as it continued to decline after 72 h, while cell growth proliferated further. Ample nutrient supply may prolong bioflocculant production with flocculating activities of 90 % and higher, while excess oxygen supply may promote rapid growth that can lead to poor flocculation due to the re-use of the bioflocculant as a substitute for food during starvation. Bioflocculant production occurred at best at 25-30 °C incubation temperature and at the initial pH medium of 7 to 8. The bioflocculant was proven to be extracellularly produced as the broth and the supernatant possessed the ability to flocculate the suspended kaolin particles. Bioflocculant productions by UPMB13 were hereditarily stable among succeeding progenies, hence, proving genetic competency. About 0.90 g of purified bioflocculant were collected from 1 L culture broth of UPMB13 under the optimized fermentation conditions. Springer Verlag 2016-06 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/54391/1/Glutamic%20acid%20independent%20production%20of%20bioflocculants%20by%20Bacillus%20subtilis%20UPMB13.pdf Zulkeflee, Zufarzaana and Shamsuddin, Zulkifli and Aris, Ahmad Zaharin and Yusoff, Mohd Kamil and Komilis, Dimitrios and Sánchez, Antoni (2016) Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13. Environmental Processes, 3 (2). pp. 353-367. ISSN 2198-7491; ESSN: 2198-7505 https://link.springer.com/article/10.1007/s40710-016-0161-3 Extracellular polymeric substance (EPS); Biopolymer; γ-PGA; De-novo; Kaolin assay 10.1007/s40710-016-0161-3 |
| spellingShingle | Extracellular polymeric substance (EPS); Biopolymer; γ-PGA; De-novo; Kaolin assay Zulkeflee, Zufarzaana Shamsuddin, Zulkifli Aris, Ahmad Zaharin Yusoff, Mohd Kamil Komilis, Dimitrios Sánchez, Antoni Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13 |
| title | Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13 |
| title_full | Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13 |
| title_fullStr | Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13 |
| title_full_unstemmed | Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13 |
| title_short | Glutamic acid independent production of bioflocculants by Bacillus subtilis UPMB13 |
| title_sort | glutamic acid independent production of bioflocculants by bacillus subtilis upmb13 |
| topic | Extracellular polymeric substance (EPS); Biopolymer; γ-PGA; De-novo; Kaolin assay |
| url | http://psasir.upm.edu.my/id/eprint/54391/1/Glutamic%20acid%20independent%20production%20of%20bioflocculants%20by%20Bacillus%20subtilis%20UPMB13.pdf |
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