Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC
Hyperlipidemia is defined as the presence of either hypertriglyceridemia or hypercholesterolemia, which could cause atherosclerosis. Although hyperlipidemia can be treated by hypolipidemic drugs, they are limited due to lack of effectiveness and safety. Previous studies demonstrated that xanthorrhiz...
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54474/1/Hypolipidemic%20activities%20of%20xanthorrhizol%20purified%20from%20centrifugal%20TLC.pdf |
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| author | Oon, Seok Fang Nallappan, Meenakshii Kassim, Nur Kartinee Shohaimi, Shamarina Sa'ariwijaya, Mohd Shazrul Fazry Tee, Thiam Tsui Cheah, Yew Hoong |
| author_facet | Oon, Seok Fang Nallappan, Meenakshii Kassim, Nur Kartinee Shohaimi, Shamarina Sa'ariwijaya, Mohd Shazrul Fazry Tee, Thiam Tsui Cheah, Yew Hoong |
| author_sort | Oon, Seok Fang |
| collection | UPM |
| description | Hyperlipidemia is defined as the presence of either hypertriglyceridemia or hypercholesterolemia, which could cause atherosclerosis. Although hyperlipidemia can be treated by hypolipidemic drugs, they are limited due to lack of effectiveness and safety. Previous studies demonstrated that xanthorrhizol (XNT) isolated from Curcuma xanthorrhizza Roxb. reduced the levels of free fatty acid and triglyceride in vivo. However, its ability to inhibit cholesterol uptake in HT29 colon cells and adipogenesis in 3T3-L1 cells are yet to be reported. In this study, XNT purified from centrifugal TLC demonstrated 98.3% purity, indicating it could be an alternative purification method. The IC50 values of XNT were 30.81 ± 0.78 μg/mL in HT29 cells and 35.07 ± 0.24 μg/mL in 3T3-L1 adipocytes, respectively. Cholesterol uptake inhibition study using HT29 colon cells showed that XNT (15 μg/mL) significantly inhibited the fluorescent cholesterol analogue NBD uptake by up to 27 ± 3.1% relative to control. On the other hand, higher concentration of XNT (50 μg/mL) significantly suppressed the growth of 3T3-L1 adipocytes (5.9 ± 0.58%) compared to 3T3-L1 preadipocytes (81.31 ± 0.55%). XNT was found to impede adipogenesis of 3T3-L1 adipocytes in a dose-dependent manner from 3.125 to 12.5 μg/mL, where 12.5 μg/mL significantly suppressed 36.13 ± 2.1% of lipid accumulation. We postulate that inhibition of cholesterol uptake, adipogenesis, preadipocyte and adipocyte number may be utilized as treatment modalities to reduce the prevalence of lipidemia. To conclude, XNT could be a potential hypolipidemic agent to improve cardiovascular health in the future. |
| first_indexed | 2024-03-06T09:20:46Z |
| format | Article |
| id | upm.eprints-54474 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:20:46Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-544742018-03-20T01:10:02Z http://psasir.upm.edu.my/id/eprint/54474/ Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC Oon, Seok Fang Nallappan, Meenakshii Kassim, Nur Kartinee Shohaimi, Shamarina Sa'ariwijaya, Mohd Shazrul Fazry Tee, Thiam Tsui Cheah, Yew Hoong Hyperlipidemia is defined as the presence of either hypertriglyceridemia or hypercholesterolemia, which could cause atherosclerosis. Although hyperlipidemia can be treated by hypolipidemic drugs, they are limited due to lack of effectiveness and safety. Previous studies demonstrated that xanthorrhizol (XNT) isolated from Curcuma xanthorrhizza Roxb. reduced the levels of free fatty acid and triglyceride in vivo. However, its ability to inhibit cholesterol uptake in HT29 colon cells and adipogenesis in 3T3-L1 cells are yet to be reported. In this study, XNT purified from centrifugal TLC demonstrated 98.3% purity, indicating it could be an alternative purification method. The IC50 values of XNT were 30.81 ± 0.78 μg/mL in HT29 cells and 35.07 ± 0.24 μg/mL in 3T3-L1 adipocytes, respectively. Cholesterol uptake inhibition study using HT29 colon cells showed that XNT (15 μg/mL) significantly inhibited the fluorescent cholesterol analogue NBD uptake by up to 27 ± 3.1% relative to control. On the other hand, higher concentration of XNT (50 μg/mL) significantly suppressed the growth of 3T3-L1 adipocytes (5.9 ± 0.58%) compared to 3T3-L1 preadipocytes (81.31 ± 0.55%). XNT was found to impede adipogenesis of 3T3-L1 adipocytes in a dose-dependent manner from 3.125 to 12.5 μg/mL, where 12.5 μg/mL significantly suppressed 36.13 ± 2.1% of lipid accumulation. We postulate that inhibition of cholesterol uptake, adipogenesis, preadipocyte and adipocyte number may be utilized as treatment modalities to reduce the prevalence of lipidemia. To conclude, XNT could be a potential hypolipidemic agent to improve cardiovascular health in the future. Elsevier 2016-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54474/1/Hypolipidemic%20activities%20of%20xanthorrhizol%20purified%20from%20centrifugal%20TLC.pdf Oon, Seok Fang and Nallappan, Meenakshii and Kassim, Nur Kartinee and Shohaimi, Shamarina and Sa'ariwijaya, Mohd Shazrul Fazry and Tee, Thiam Tsui and Cheah, Yew Hoong (2016) Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC. Biochemical and Biophysical Research Communications, 478 (3). pp. 1403-1408. ISSN 0006-291X; ESSN: 1090-2104 https://www.sciencedirect.com/science/article/pii/S0006291X16314000 Xanthorrhizol; Centrifugal TLC; HT29 colon cells; 3T3-L1 adipocytes; Cholesterol; Adipogenesis 10.1016/j.bbrc.2016.08.136 |
| spellingShingle | Xanthorrhizol; Centrifugal TLC; HT29 colon cells; 3T3-L1 adipocytes; Cholesterol; Adipogenesis Oon, Seok Fang Nallappan, Meenakshii Kassim, Nur Kartinee Shohaimi, Shamarina Sa'ariwijaya, Mohd Shazrul Fazry Tee, Thiam Tsui Cheah, Yew Hoong Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC |
| title | Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC |
| title_full | Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC |
| title_fullStr | Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC |
| title_full_unstemmed | Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC |
| title_short | Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC |
| title_sort | hypolipidemic activities of xanthorrhizol purified from centrifugal tlc |
| topic | Xanthorrhizol; Centrifugal TLC; HT29 colon cells; 3T3-L1 adipocytes; Cholesterol; Adipogenesis |
| url | http://psasir.upm.edu.my/id/eprint/54474/1/Hypolipidemic%20activities%20of%20xanthorrhizol%20purified%20from%20centrifugal%20TLC.pdf |
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