Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil
The purpose of the study was to identify effective bacteria that degrade phenanthrene and pyrene from used engine oil contaminated soil. A total of 93 bacterial isolates were obtained and their degradations were determined. Based on culture-depended technique and tested for in vitro phenanthrene and...
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| Format: | Article |
| Language: | English |
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IJSER Publications
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54486/1/Identification%20of%20phenanthrene%20and%20pyrene%20degrading%20bacteria%20from%20used%20engine%20oil%20contaminated%20soil.pdf |
| _version_ | 1825931046950010880 |
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| author | Darma, Umar Zubairu Abd Aziz, Nor Azwady Zulkefli, Syaizwan Zahmir Mustafa, Muskhazli |
| author_facet | Darma, Umar Zubairu Abd Aziz, Nor Azwady Zulkefli, Syaizwan Zahmir Mustafa, Muskhazli |
| author_sort | Darma, Umar Zubairu |
| collection | UPM |
| description | The purpose of the study was to identify effective bacteria that degrade phenanthrene and pyrene from used engine oil contaminated soil. A total of 93 bacterial isolates were obtained and their degradations were determined. Based on culture-depended technique and tested for in vitro phenanthrene and pyrene degradations using spray plate technique and colorimetric assay with 2,6-dichloroindophenol (DCPIP). Fifty three isolates shows evidence of 5 g/l phenanthrene degradation within 72 hours in spray plate technique. Further 23 isolates were selected from 53 isolates after the colorimetric screening. Biodegradation study shows two isolates named MM045 and MM087 with 75.2% and 80.2% degradations for 500 mg/l phenanthrene and also 54.3% and 59.7% of 250 mg/l pyrene degradations within 24 hours respectively. These isolates were identified as Cronobacter sakazakii strain MM045 (Accession number: KT933253) and Enterobacter specie strain MM087 (Accession number: KT933254) using 16S rRNA gene sequencing and neighbour joining tree analyses. The identified isolates were proven to be fast effective in degrading high concentrations of phenanthrene and pyrene as their sole carbon and energy sources. |
| first_indexed | 2024-03-06T09:20:48Z |
| format | Article |
| id | upm.eprints-54486 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:20:48Z |
| publishDate | 2016 |
| publisher | IJSER Publications |
| record_format | dspace |
| spelling | upm.eprints-544862018-03-21T01:50:25Z http://psasir.upm.edu.my/id/eprint/54486/ Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil Darma, Umar Zubairu Abd Aziz, Nor Azwady Zulkefli, Syaizwan Zahmir Mustafa, Muskhazli The purpose of the study was to identify effective bacteria that degrade phenanthrene and pyrene from used engine oil contaminated soil. A total of 93 bacterial isolates were obtained and their degradations were determined. Based on culture-depended technique and tested for in vitro phenanthrene and pyrene degradations using spray plate technique and colorimetric assay with 2,6-dichloroindophenol (DCPIP). Fifty three isolates shows evidence of 5 g/l phenanthrene degradation within 72 hours in spray plate technique. Further 23 isolates were selected from 53 isolates after the colorimetric screening. Biodegradation study shows two isolates named MM045 and MM087 with 75.2% and 80.2% degradations for 500 mg/l phenanthrene and also 54.3% and 59.7% of 250 mg/l pyrene degradations within 24 hours respectively. These isolates were identified as Cronobacter sakazakii strain MM045 (Accession number: KT933253) and Enterobacter specie strain MM087 (Accession number: KT933254) using 16S rRNA gene sequencing and neighbour joining tree analyses. The identified isolates were proven to be fast effective in degrading high concentrations of phenanthrene and pyrene as their sole carbon and energy sources. IJSER Publications 2016-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54486/1/Identification%20of%20phenanthrene%20and%20pyrene%20degrading%20bacteria%20from%20used%20engine%20oil%20contaminated%20soil.pdf Darma, Umar Zubairu and Abd Aziz, Nor Azwady and Zulkefli, Syaizwan Zahmir and Mustafa, Muskhazli (2016) Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil. International Journal of Scientific & Engineering Research, 7 (3). pp. 680-686. ISSN 2229-5518 https://www.ijser.org/paper/Identification-of-Phenanthrene-and-Pyrene-degrading-Bacteria-from-used-Engine-Oil-contaminated-Soil.html Biodegradation; Contaminated soil; Phenanthrene; Pyrene |
| spellingShingle | Biodegradation; Contaminated soil; Phenanthrene; Pyrene Darma, Umar Zubairu Abd Aziz, Nor Azwady Zulkefli, Syaizwan Zahmir Mustafa, Muskhazli Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil |
| title | Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil |
| title_full | Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil |
| title_fullStr | Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil |
| title_full_unstemmed | Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil |
| title_short | Identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil |
| title_sort | identification of phenanthrene and pyrene degrading bacteria from used engine oil contaminated soil |
| topic | Biodegradation; Contaminated soil; Phenanthrene; Pyrene |
| url | http://psasir.upm.edu.my/id/eprint/54486/1/Identification%20of%20phenanthrene%20and%20pyrene%20degrading%20bacteria%20from%20used%20engine%20oil%20contaminated%20soil.pdf |
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