Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum)

Pepino (Solanum muricatum var. pepino) plants were found affected by an extensive leaf spot caused by plant pathogenic fungi during a survey in the Cameron highlands, Pahang state, Malaysia. Symptomatic leaf samples were collected from infected pepino plants and cultivated on PDA medium, and the pat...

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Main Authors: Nasehi, Abbas, Kadir, Jugah, Esfahani, Mehdi Nasr, Ashtiani, Farnaz Abed, Golkhandan, Elham, Ashkani, Sadegh
Format: Article
Language:English
Published: Blackwell Verlag GmbH 2016
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/54488/1/Identification%20of%20the%20new%20pathogen%20%28Stemphylium%20lycopersici%29%20causing%20leaf%20spot%20on%20pepino%20%28Solanum%20muricatum%29%20.pdf
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author Nasehi, Abbas
Kadir, Jugah
Esfahani, Mehdi Nasr
Ashtiani, Farnaz Abed
Golkhandan, Elham
Ashkani, Sadegh
author_facet Nasehi, Abbas
Kadir, Jugah
Esfahani, Mehdi Nasr
Ashtiani, Farnaz Abed
Golkhandan, Elham
Ashkani, Sadegh
author_sort Nasehi, Abbas
collection UPM
description Pepino (Solanum muricatum var. pepino) plants were found affected by an extensive leaf spot caused by plant pathogenic fungi during a survey in the Cameron highlands, Pahang state, Malaysia. Symptomatic leaf samples were collected from infected pepino plants and cultivated on PDA medium, and the pathogen was isolated and purified; then, consequently, all isolates were identified as Stemphylium lycopersici on the basis of their cultural and morphological characteristics and combined sequences of the internal transcribed spacer (ITS) and glyceraldehyde‐3‐phosphate dehydrogenase (gpd) regions. A pathogenicity assay on detached leaves further confirmed that S. lycopersici causes leaf spot disease. To the best of our knowledge, this is the first report of S. lycopersici causing leaf spot on pepino in Malaysia and worldwide.
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spelling upm.eprints-544882018-03-21T02:36:09Z http://psasir.upm.edu.my/id/eprint/54488/ Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum) Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Ashtiani, Farnaz Abed Golkhandan, Elham Ashkani, Sadegh Pepino (Solanum muricatum var. pepino) plants were found affected by an extensive leaf spot caused by plant pathogenic fungi during a survey in the Cameron highlands, Pahang state, Malaysia. Symptomatic leaf samples were collected from infected pepino plants and cultivated on PDA medium, and the pathogen was isolated and purified; then, consequently, all isolates were identified as Stemphylium lycopersici on the basis of their cultural and morphological characteristics and combined sequences of the internal transcribed spacer (ITS) and glyceraldehyde‐3‐phosphate dehydrogenase (gpd) regions. A pathogenicity assay on detached leaves further confirmed that S. lycopersici causes leaf spot disease. To the best of our knowledge, this is the first report of S. lycopersici causing leaf spot on pepino in Malaysia and worldwide. Blackwell Verlag GmbH 2016-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54488/1/Identification%20of%20the%20new%20pathogen%20%28Stemphylium%20lycopersici%29%20causing%20leaf%20spot%20on%20pepino%20%28Solanum%20muricatum%29%20.pdf Nasehi, Abbas and Kadir, Jugah and Esfahani, Mehdi Nasr and Ashtiani, Farnaz Abed and Golkhandan, Elham and Ashkani, Sadegh (2016) Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum). Journal of Phytopathology, 164 (6). pp. 421-426. ISSN 0931-1785; ESSN: 1439-0434 https://onlinelibrary.wiley.com/doi/abs/10.1111/jph.12431 Leaf spot; Pepino; Phylogeny; Solanum muricatum var. pepino; Stemphylium lycopersici 10.1111/jph.12431
spellingShingle Leaf spot; Pepino; Phylogeny; Solanum muricatum var. pepino; Stemphylium lycopersici
Nasehi, Abbas
Kadir, Jugah
Esfahani, Mehdi Nasr
Ashtiani, Farnaz Abed
Golkhandan, Elham
Ashkani, Sadegh
Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum)
title Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum)
title_full Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum)
title_fullStr Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum)
title_full_unstemmed Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum)
title_short Identification of the new pathogen (Stemphylium lycopersici) causing leaf spot on pepino (Solanum muricatum)
title_sort identification of the new pathogen stemphylium lycopersici causing leaf spot on pepino solanum muricatum
topic Leaf spot; Pepino; Phylogeny; Solanum muricatum var. pepino; Stemphylium lycopersici
url http://psasir.upm.edu.my/id/eprint/54488/1/Identification%20of%20the%20new%20pathogen%20%28Stemphylium%20lycopersici%29%20causing%20leaf%20spot%20on%20pepino%20%28Solanum%20muricatum%29%20.pdf
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