Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes
The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expre...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The Korean Society of Veterinary Science
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54518/1/Improved%20immunogenicity%20of%20Newcastle%20disease%20virus%20inactivated%20vaccine%20.pdf |
| _version_ | 1796976097980579840 |
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| author | Firouzamandi, Masoumeh Moeini, Hassan Hosseini, Davood Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh Ideris, Aini |
| author_facet | Firouzamandi, Masoumeh Moeini, Hassan Hosseini, Davood Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh Ideris, Aini |
| author_sort | Firouzamandi, Masoumeh |
| collection | UPM |
| description | The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant difference in enzyme-linked immunosorbent assay antibody levels (p < 0.05) elicited by either pIRES/F, pIRES/F+ pIRES/HN or pIRES-F/HN at one week after the booster in specific pathogen free chickens when compared with the inactivated vaccine alone. Taken together, these results indicated that recombinant pDNA could be used to increase the efficacy of the inactivated vaccine immunization procedure. |
| first_indexed | 2024-03-06T09:20:54Z |
| format | Article |
| id | upm.eprints-54518 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:20:54Z |
| publishDate | 2016 |
| publisher | The Korean Society of Veterinary Science |
| record_format | dspace |
| spelling | upm.eprints-545182020-05-28T04:23:25Z http://psasir.upm.edu.my/id/eprint/54518/ Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes Firouzamandi, Masoumeh Moeini, Hassan Hosseini, Davood Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh Ideris, Aini The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant difference in enzyme-linked immunosorbent assay antibody levels (p < 0.05) elicited by either pIRES/F, pIRES/F+ pIRES/HN or pIRES-F/HN at one week after the booster in specific pathogen free chickens when compared with the inactivated vaccine alone. Taken together, these results indicated that recombinant pDNA could be used to increase the efficacy of the inactivated vaccine immunization procedure. The Korean Society of Veterinary Science 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54518/1/Improved%20immunogenicity%20of%20Newcastle%20disease%20virus%20inactivated%20vaccine%20.pdf Firouzamandi, Masoumeh and Moeini, Hassan and Hosseini, Davood and Bejo, Mohd Hair and Omar, Abdul Rahman and Mehrbod, Parvaneh and Ideris, Aini (2016) Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes. Journal of Veterinary Science, 17 (1). pp. 21-26. ISSN 1229-845X; ESSN: 1976-555X https://vetsci.org/search.php?where=aview&id=10.4142/jvs.2016.17.1.21&code=0118JVS&vmode=AONLY 10.4142/jvs.2016.17.1.21 |
| spellingShingle | Firouzamandi, Masoumeh Moeini, Hassan Hosseini, Davood Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh Ideris, Aini Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes |
| title | Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes |
| title_full | Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes |
| title_fullStr | Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes |
| title_full_unstemmed | Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes |
| title_short | Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes |
| title_sort | improved immunogenicity of newcastle disease virus inactivated vaccine following dna vaccination using newcastle disease virus hemagglutinin neuraminidase and fusion protein genes |
| url | http://psasir.upm.edu.my/id/eprint/54518/1/Improved%20immunogenicity%20of%20Newcastle%20disease%20virus%20inactivated%20vaccine%20.pdf |
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