Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats
This study attempts to develop an experimental gestational diabetes mellitus (GDM) animal model in female Sprague-Dawley rats. Rats were fed with high fat sucrose diet, impregnated, and induced with Streptozotocin and Nicotinamide on gestational day 0 (D0). Sleeping patterns of the rats were also ma...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55066/1/Animal%20Model%20of%20Gestational%20Diabetes%20Mellitus.pdf |
| _version_ | 1825931147992891392 |
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| author | Abdul Aziz, Siti Hajar John, Cini Mathew Mohamed Yusof, Nur Intan Saidaah Nordin, Massita Ramasamy, Rajesh Adam, Aishah Mohd Fauzi, Fazlin |
| author_facet | Abdul Aziz, Siti Hajar John, Cini Mathew Mohamed Yusof, Nur Intan Saidaah Nordin, Massita Ramasamy, Rajesh Adam, Aishah Mohd Fauzi, Fazlin |
| author_sort | Abdul Aziz, Siti Hajar |
| collection | UPM |
| description | This study attempts to develop an experimental gestational diabetes mellitus (GDM) animal model in female Sprague-Dawley rats. Rats were fed with high fat sucrose diet, impregnated, and induced with Streptozotocin and Nicotinamide on gestational day 0 (D0). Sleeping patterns of the rats were also manipulated to induce stress, a lifestyle factor that contributes to GDM. Rats were tested for glycemic parameters (glucose, C-peptide, and insulin), lipid profiles (total cholesterol, triglycerides, HDL, and LDL), genes affecting insulin signaling (IRS-2, AKT-1, and PCK-1), glucose transporters (GLUT-2 and GLUT-4), proinflammatory cytokines (IL-6, TNF-α), and antioxidants (SOD, CAT, and GPX) on D6 and D21. GDM rats showed possible insulin resistance as evidenced by high expression of proinflammatory cytokines, PCK-1 and CRP. Furthermore, low levels of IRS-2 and AKT-1 genes and downregulation of GLUT-4 from the initial to final phases indicate possible defect of insulin signaling. GDM rats also showed an impairment of antioxidant status and a hyperlipidemic state. Additionally, GDM rats exhibited significantly higher body weight and blood glucose and lower plasma insulin level and C-peptide than control. Based on the findings outlined, the current GDM animal model closely replicates the disease state in human and can serve as a reference for future investigations. |
| first_indexed | 2024-03-06T09:22:20Z |
| format | Article |
| id | upm.eprints-55066 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:22:20Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-550662018-07-13T08:57:42Z http://psasir.upm.edu.my/id/eprint/55066/ Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats Abdul Aziz, Siti Hajar John, Cini Mathew Mohamed Yusof, Nur Intan Saidaah Nordin, Massita Ramasamy, Rajesh Adam, Aishah Mohd Fauzi, Fazlin This study attempts to develop an experimental gestational diabetes mellitus (GDM) animal model in female Sprague-Dawley rats. Rats were fed with high fat sucrose diet, impregnated, and induced with Streptozotocin and Nicotinamide on gestational day 0 (D0). Sleeping patterns of the rats were also manipulated to induce stress, a lifestyle factor that contributes to GDM. Rats were tested for glycemic parameters (glucose, C-peptide, and insulin), lipid profiles (total cholesterol, triglycerides, HDL, and LDL), genes affecting insulin signaling (IRS-2, AKT-1, and PCK-1), glucose transporters (GLUT-2 and GLUT-4), proinflammatory cytokines (IL-6, TNF-α), and antioxidants (SOD, CAT, and GPX) on D6 and D21. GDM rats showed possible insulin resistance as evidenced by high expression of proinflammatory cytokines, PCK-1 and CRP. Furthermore, low levels of IRS-2 and AKT-1 genes and downregulation of GLUT-4 from the initial to final phases indicate possible defect of insulin signaling. GDM rats also showed an impairment of antioxidant status and a hyperlipidemic state. Additionally, GDM rats exhibited significantly higher body weight and blood glucose and lower plasma insulin level and C-peptide than control. Based on the findings outlined, the current GDM animal model closely replicates the disease state in human and can serve as a reference for future investigations. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/55066/1/Animal%20Model%20of%20Gestational%20Diabetes%20Mellitus.pdf Abdul Aziz, Siti Hajar and John, Cini Mathew and Mohamed Yusof, Nur Intan Saidaah and Nordin, Massita and Ramasamy, Rajesh and Adam, Aishah and Mohd Fauzi, Fazlin (2016) Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats. BioMed Research International, 2016 (9704607). pp. 1-14. ISSN 2314-6133; ESSN: 2314-6141 10.1155/2016/9704607 |
| spellingShingle | Abdul Aziz, Siti Hajar John, Cini Mathew Mohamed Yusof, Nur Intan Saidaah Nordin, Massita Ramasamy, Rajesh Adam, Aishah Mohd Fauzi, Fazlin Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats |
| title | Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats |
| title_full | Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats |
| title_fullStr | Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats |
| title_full_unstemmed | Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats |
| title_short | Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats |
| title_sort | animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors nutritional pharmacological and stress in rats |
| url | http://psasir.upm.edu.my/id/eprint/55066/1/Animal%20Model%20of%20Gestational%20Diabetes%20Mellitus.pdf |
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