Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment
This study investigates the production of biopesticide based on Bacillus thuringiensis activity in culture media supplemented with semi-solid wastewater sludge as one of the raw ingredient. A series of testing using mixture of sludge and source of protein as B. thuringiensis growth media were carrie...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55183/1/Bacillus%20thuringiensis%20entomotoxicity%20activity%20in%20wastewater%20sludge-culture%20medium%20towards%20Bactrocera%20dorsalis%20and%20their%20histopathological%20assessment.pdf |
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| author | M. G., Mona Fatin Syazwanee U. A., Noormasshela A. A., Nor Azwady G., Rusea M., Muskhazli |
| author_facet | M. G., Mona Fatin Syazwanee U. A., Noormasshela A. A., Nor Azwady G., Rusea M., Muskhazli |
| author_sort | M. G., Mona Fatin Syazwanee |
| collection | UPM |
| description | This study investigates the production of biopesticide based on Bacillus thuringiensis activity in culture media supplemented with semi-solid wastewater sludge as one of the raw ingredient. A series of testing using mixture of sludge and source of protein as B. thuringiensis growth media were carried out and selection of media was based on viable spore count. The entomotoxicity test of B. thuringiensis was carried out against larvae of fruit fly using diet incorporation method. Further impact of entomotoxicity was observed based on histology deformities on columnar epithelial cell and goblet cell of the midgut. A mixture of sludge with 60% wheat bran produced up to 1.64 × 10
10CFU/mL of viable spore count within 10 days of incubation. Based on entomotoxicity test, incorporation of 12 mL of semi-solid wastewater sludge-culture media into fruit fly artificial diet caused the highest fruit fly mortality at 64.8%. The value of semi-solid wastewater sludge-culture media concentration for LC50 was determined at 8.43%. Effect of entomotoxicity can be seen started from 3rd instar larvae where histopathological studies showed that up to 10% of columnar epithelial cells in the intestine were swollen and severe reduction of goblet cell’s size. Thus, it decreases the survivality of the fruit fly larvae. The present study indicated that semi-solid wastewater sludge has the potential to enhance B. thuringiensis entomotoxicity activity. |
| first_indexed | 2024-03-06T09:22:41Z |
| format | Article |
| id | upm.eprints-55183 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:22:41Z |
| publishDate | 2016 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | upm.eprints-551832018-05-15T06:35:02Z http://psasir.upm.edu.my/id/eprint/55183/ Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment M. G., Mona Fatin Syazwanee U. A., Noormasshela A. A., Nor Azwady G., Rusea M., Muskhazli This study investigates the production of biopesticide based on Bacillus thuringiensis activity in culture media supplemented with semi-solid wastewater sludge as one of the raw ingredient. A series of testing using mixture of sludge and source of protein as B. thuringiensis growth media were carried out and selection of media was based on viable spore count. The entomotoxicity test of B. thuringiensis was carried out against larvae of fruit fly using diet incorporation method. Further impact of entomotoxicity was observed based on histology deformities on columnar epithelial cell and goblet cell of the midgut. A mixture of sludge with 60% wheat bran produced up to 1.64 × 10 10CFU/mL of viable spore count within 10 days of incubation. Based on entomotoxicity test, incorporation of 12 mL of semi-solid wastewater sludge-culture media into fruit fly artificial diet caused the highest fruit fly mortality at 64.8%. The value of semi-solid wastewater sludge-culture media concentration for LC50 was determined at 8.43%. Effect of entomotoxicity can be seen started from 3rd instar larvae where histopathological studies showed that up to 10% of columnar epithelial cells in the intestine were swollen and severe reduction of goblet cell’s size. Thus, it decreases the survivality of the fruit fly larvae. The present study indicated that semi-solid wastewater sludge has the potential to enhance B. thuringiensis entomotoxicity activity. Penerbit Universiti Kebangsaan Malaysia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55183/1/Bacillus%20thuringiensis%20entomotoxicity%20activity%20in%20wastewater%20sludge-culture%20medium%20towards%20Bactrocera%20dorsalis%20and%20their%20histopathological%20assessment.pdf M. G., Mona Fatin Syazwanee and U. A., Noormasshela and A. A., Nor Azwady and G., Rusea and M., Muskhazli (2016) Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment. Sains Malaysiana, 45 (4). pp. 589-594. ISSN 0026-6039 http://www.ukm.my/jsm/ |
| spellingShingle | M. G., Mona Fatin Syazwanee U. A., Noormasshela A. A., Nor Azwady G., Rusea M., Muskhazli Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment |
| title | Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment |
| title_full | Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment |
| title_fullStr | Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment |
| title_full_unstemmed | Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment |
| title_short | Bacillus thuringiensis entomotoxicity activity in wastewater sludge-culture medium towards Bactrocera dorsalis and their histopathological assessment |
| title_sort | bacillus thuringiensis entomotoxicity activity in wastewater sludge culture medium towards bactrocera dorsalis and their histopathological assessment |
| url | http://psasir.upm.edu.my/id/eprint/55183/1/Bacillus%20thuringiensis%20entomotoxicity%20activity%20in%20wastewater%20sludge-culture%20medium%20towards%20Bactrocera%20dorsalis%20and%20their%20histopathological%20assessment.pdf |
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