Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense
Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse condit...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Society of Microbiologists
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/55190/1/Basidiospore%20and%20protoplast%20regeneration%20from%20raised%20fruiting%20bodies%20of%20pathogenic%20Ganoderma%20boninense.pdf |
| _version_ | 1825931172447780864 |
|---|---|
| author | Govender, Nisha Thopla Mahmood, Maziah Abu Seman, Idris Wong, Mui Yun |
| author_facet | Govender, Nisha Thopla Mahmood, Maziah Abu Seman, Idris Wong, Mui Yun |
| author_sort | Govender, Nisha Thopla |
| collection | UPM |
| description | Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 x 104 basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma harzianum (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 x 106 single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period). |
| first_indexed | 2024-03-06T09:22:43Z |
| format | Article |
| id | upm.eprints-55190 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:22:43Z |
| publishDate | 2016 |
| publisher | Polish Society of Microbiologists |
| record_format | dspace |
| spelling | upm.eprints-551902017-12-19T01:56:42Z http://psasir.upm.edu.my/id/eprint/55190/ Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense Govender, Nisha Thopla Mahmood, Maziah Abu Seman, Idris Wong, Mui Yun Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 x 104 basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma harzianum (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 x 106 single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period). Polish Society of Microbiologists 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55190/1/Basidiospore%20and%20protoplast%20regeneration%20from%20raised%20fruiting%20bodies%20of%20pathogenic%20Ganoderma%20boninense.pdf Govender, Nisha Thopla and Mahmood, Maziah and Abu Seman, Idris and Wong, Mui Yun (2016) Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense. Polish Journal of Microbiology, 65 (3). pp. 383-388. ISSN 1733-1331 http://www.pjm.microbiology.pl 10.5604/17331331.1215619 |
| spellingShingle | Govender, Nisha Thopla Mahmood, Maziah Abu Seman, Idris Wong, Mui Yun Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense |
| title | Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense |
| title_full | Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense |
| title_fullStr | Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense |
| title_full_unstemmed | Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense |
| title_short | Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense |
| title_sort | basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic ganoderma boninense |
| url | http://psasir.upm.edu.my/id/eprint/55190/1/Basidiospore%20and%20protoplast%20regeneration%20from%20raised%20fruiting%20bodies%20of%20pathogenic%20Ganoderma%20boninense.pdf |
| work_keys_str_mv | AT govendernishathopla basidiosporeandprotoplastregenerationfromraisedfruitingbodiesofpathogenicganodermaboninense AT mahmoodmaziah basidiosporeandprotoplastregenerationfromraisedfruitingbodiesofpathogenicganodermaboninense AT abusemanidris basidiosporeandprotoplastregenerationfromraisedfruitingbodiesofpathogenicganodermaboninense AT wongmuiyun basidiosporeandprotoplastregenerationfromraisedfruitingbodiesofpathogenicganodermaboninense |