Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia
The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) includes important destructive pathogens causing blast disease on various species from the family Poaceae. Thirty-five Magnaporthe isolates were collected from diseased rice plants (variety MR219) in different rice-growing regions of Malaysi...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55248/1/Characterisation%20of%20Magnaporthe%20oryzae%20isolates%20from%20rice%20in%20peninsular%20Malaysia.pdf |
| _version_ | 1796976232784461824 |
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| author | Abed-Ashtiani, Farnaz Kadir, Jugah Nasehi, Abbas Reza, Seyed Hashemian, Rahaghi Vadamalai, Ganesan Rambe, Siti Khadijah |
| author_facet | Abed-Ashtiani, Farnaz Kadir, Jugah Nasehi, Abbas Reza, Seyed Hashemian, Rahaghi Vadamalai, Ganesan Rambe, Siti Khadijah |
| author_sort | Abed-Ashtiani, Farnaz |
| collection | UPM |
| description | The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) includes important destructive pathogens causing
blast disease on various species from the family Poaceae. Thirty-five Magnaporthe isolates were collected from diseased rice plants (variety MR219) in different rice-growing regions of Malaysia, including the five states of Selangor,Penang, Kedah, Kelantan, and Perak between 2010 and 2014. DNA sequence analyses of the internal transcribed spacer (ITS), actin, β-tubulin and calmodulin gene regions, random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) analyses were conducted to analyse 35 Magnaporthe isolates. Phylogenetic analysis of the combined dataset confirmed the identification of all isolates as M. oryzae with a high distance from other Magnaporthe (Pyricularia) species. RAPD and ISSR analyses indicated the existence of a relatively low similarity index value among M. oryzae isolates through identification of four main clades. The clustering of RAPD and ISSR analyses demonstrated that there was a correlation between the isolates and their geographical origins. |
| first_indexed | 2024-03-06T09:22:52Z |
| format | Article |
| id | upm.eprints-55248 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:22:52Z |
| publishDate | 2016 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | dspace |
| spelling | upm.eprints-552482018-01-22T08:28:18Z http://psasir.upm.edu.my/id/eprint/55248/ Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia Abed-Ashtiani, Farnaz Kadir, Jugah Nasehi, Abbas Reza, Seyed Hashemian, Rahaghi Vadamalai, Ganesan Rambe, Siti Khadijah The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) includes important destructive pathogens causing blast disease on various species from the family Poaceae. Thirty-five Magnaporthe isolates were collected from diseased rice plants (variety MR219) in different rice-growing regions of Malaysia, including the five states of Selangor,Penang, Kedah, Kelantan, and Perak between 2010 and 2014. DNA sequence analyses of the internal transcribed spacer (ITS), actin, β-tubulin and calmodulin gene regions, random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) analyses were conducted to analyse 35 Magnaporthe isolates. Phylogenetic analysis of the combined dataset confirmed the identification of all isolates as M. oryzae with a high distance from other Magnaporthe (Pyricularia) species. RAPD and ISSR analyses indicated the existence of a relatively low similarity index value among M. oryzae isolates through identification of four main clades. The clustering of RAPD and ISSR analyses demonstrated that there was a correlation between the isolates and their geographical origins. Czech Academy of Agricultural Sciences 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55248/1/Characterisation%20of%20Magnaporthe%20oryzae%20isolates%20from%20rice%20in%20peninsular%20Malaysia.pdf Abed-Ashtiani, Farnaz and Kadir, Jugah and Nasehi, Abbas and Reza, Seyed and Hashemian, Rahaghi and Vadamalai, Ganesan and Rambe, Siti Khadijah (2016) Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia. Czech Journal of Genetics and Plant Breeding, 52 (4). pp. 145-156. ISSN 1212-1975; ESSN: 1805-9325 10.17221/102/2016-CJGPB |
| spellingShingle | Abed-Ashtiani, Farnaz Kadir, Jugah Nasehi, Abbas Reza, Seyed Hashemian, Rahaghi Vadamalai, Ganesan Rambe, Siti Khadijah Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia |
| title | Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia |
| title_full | Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia |
| title_fullStr | Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia |
| title_full_unstemmed | Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia |
| title_short | Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia |
| title_sort | characterisation of magnaporthe oryzae isolates from rice in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/55248/1/Characterisation%20of%20Magnaporthe%20oryzae%20isolates%20from%20rice%20in%20peninsular%20Malaysia.pdf |
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