Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts
The purpose of this study was to investigate the effect of dietary lecithin on skeletal muscle gene expression of collagen precursors and enzymes involved in collagen synthesis and degradation. Finisher gilts with an average start weight of 55.9 ± 2.22 kg were fed diets containing either 0, 4, 20 or...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute (MDPI)
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55517/1/Dietary%20Lecithin%20Decreases%20Skeletal%20Muscle%20COL1A1%20and%20COL3A1%20Gene%20Expression%20in%20Finisher%20Gilts.pdf |
| _version_ | 1796976290119548928 |
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| author | Akit, Henny Collins, Cherie Fahri, Fahri T. Hung, Alex D'Souza , Darryl Nicholas Leury, Brian J. Dunshea, Frank Rowland |
| author_facet | Akit, Henny Collins, Cherie Fahri, Fahri T. Hung, Alex D'Souza , Darryl Nicholas Leury, Brian J. Dunshea, Frank Rowland |
| author_sort | Akit, Henny |
| collection | UPM |
| description | The purpose of this study was to investigate the effect of dietary lecithin on skeletal muscle gene expression of collagen precursors and enzymes involved in collagen synthesis and degradation. Finisher gilts with an average start weight of 55.9 ± 2.22 kg were fed diets containing either 0, 4, 20 or 80 g/kg soybean lecithin prior to harvest for six weeks and the rectus abdominis muscle gene expression profile was analyzed by quantitative real-time PCR. Lecithin treatment down-regulated Type I (α1) procollagen (COL1A1) and Type III (α1) procollagen (COL3A1) mRNA expression (p < 0.05, respectively), indicating a decrease in the precursors for collagen synthesis. The α-subunit of prolyl 4-hydroxylase (P4H) mRNA expression also tended to be down-regulated (p = 0.056), indicating a decrease in collagen synthesis. Decreased matrix metalloproteinase-1 (MMP-1) mRNA expression may reflect a positive regulatory response to the reduced collagen synthesis in muscle from the pigs fed lecithin (p = 0.035). Lecithin had no effect on tissue inhibitor metalloproteinase-1 (TIMP-1), matrix metalloproteinase-13 (MMP-13) and lysyl oxidase mRNA expression. In conclusion, lecithin down-regulated COL1A1 and COL3A1 as well as tended to down-regulate α-subunit P4H expression. However, determination of muscle collagen content and solubility are required to support the gene functions. |
| first_indexed | 2024-03-06T09:23:39Z |
| format | Article |
| id | upm.eprints-55517 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:23:39Z |
| publishDate | 2016 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| record_format | dspace |
| spelling | upm.eprints-555172017-09-14T02:22:00Z http://psasir.upm.edu.my/id/eprint/55517/ Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts Akit, Henny Collins, Cherie Fahri, Fahri T. Hung, Alex D'Souza , Darryl Nicholas Leury, Brian J. Dunshea, Frank Rowland The purpose of this study was to investigate the effect of dietary lecithin on skeletal muscle gene expression of collagen precursors and enzymes involved in collagen synthesis and degradation. Finisher gilts with an average start weight of 55.9 ± 2.22 kg were fed diets containing either 0, 4, 20 or 80 g/kg soybean lecithin prior to harvest for six weeks and the rectus abdominis muscle gene expression profile was analyzed by quantitative real-time PCR. Lecithin treatment down-regulated Type I (α1) procollagen (COL1A1) and Type III (α1) procollagen (COL3A1) mRNA expression (p < 0.05, respectively), indicating a decrease in the precursors for collagen synthesis. The α-subunit of prolyl 4-hydroxylase (P4H) mRNA expression also tended to be down-regulated (p = 0.056), indicating a decrease in collagen synthesis. Decreased matrix metalloproteinase-1 (MMP-1) mRNA expression may reflect a positive regulatory response to the reduced collagen synthesis in muscle from the pigs fed lecithin (p = 0.035). Lecithin had no effect on tissue inhibitor metalloproteinase-1 (TIMP-1), matrix metalloproteinase-13 (MMP-13) and lysyl oxidase mRNA expression. In conclusion, lecithin down-regulated COL1A1 and COL3A1 as well as tended to down-regulate α-subunit P4H expression. However, determination of muscle collagen content and solubility are required to support the gene functions. Multidisciplinary Digital Publishing Institute (MDPI) 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55517/1/Dietary%20Lecithin%20Decreases%20Skeletal%20Muscle%20COL1A1%20and%20COL3A1%20Gene%20Expression%20in%20Finisher%20Gilts.pdf Akit, Henny and Collins, Cherie and Fahri, Fahri T. and Hung, Alex and D'Souza , Darryl Nicholas and Leury, Brian J. and Dunshea, Frank Rowland (2016) Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts. Animals, 6 (6). pp. 1-8. ISSN 2076-2615 http://www.mdpi.com/2076-2615/6/6/38 10.3390/ani6060038 |
| spellingShingle | Akit, Henny Collins, Cherie Fahri, Fahri T. Hung, Alex D'Souza , Darryl Nicholas Leury, Brian J. Dunshea, Frank Rowland Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts |
| title | Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts |
| title_full | Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts |
| title_fullStr | Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts |
| title_full_unstemmed | Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts |
| title_short | Dietary lecithin decreases skeletal muscle COL1A1 and COL3A1 gene expression in finisher gilts |
| title_sort | dietary lecithin decreases skeletal muscle col1a1 and col3a1 gene expression in finisher gilts |
| url | http://psasir.upm.edu.my/id/eprint/55517/1/Dietary%20Lecithin%20Decreases%20Skeletal%20Muscle%20COL1A1%20and%20COL3A1%20Gene%20Expression%20in%20Finisher%20Gilts.pdf |
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