Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos
The impacts of environmental stressors on polyploid organisms are largely unknown. This study investigated changes in morphometric, molecular, and biochemical parameters in full-sibling diploid and triploid African catfish (Clarias gariepinus) in response to chlorpyrifos (CPF) exposures. Juvenile fi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55532/1/Diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20differ%20in%20biomarker%20responses%20.pdf |
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| author | Karami, Ali Goh, Yong Meng Jahromi, Mohammad Faseleh James Lazorchak, Ali Abdullah, Maha Courtenay, Simon C. |
| author_facet | Karami, Ali Goh, Yong Meng Jahromi, Mohammad Faseleh James Lazorchak, Ali Abdullah, Maha Courtenay, Simon C. |
| author_sort | Karami, Ali |
| collection | UPM |
| description | The impacts of environmental stressors on polyploid organisms are largely unknown. This study investigated changes in morphometric, molecular, and biochemical parameters in full-sibling diploid and triploid African catfish (Clarias gariepinus) in response to chlorpyrifos (CPF) exposures. Juvenile fish were exposed to three concentrations of CPF (mean measured μg/L (SD): 9.71 (2.27), 15.7 (3.69), 31.21 (5.04)) under a static-renewal condition for 21 days. Diploid control groups had higher hepatosomatic index (HSI), plasma testosterone (T), and brain GnRH and cyp19a2 expression levels than triploids. In CPF-exposed groups, changes in HSI, total weight and length were different between the diploid and triploid fish. In contrast, condition factor did not alter in any of the treatments, while visceral-somatic index (VSI) changed only in diploids. In diploid fish, exposure to CPF did not change brain 11β-hsd2, ftz-f1, foxl2, GnRH or cyp19a2 mRNA levels, while reduced tph2 transcript levels compared to the control group. In contrast, 11β-hsd2 and foxl2 expression levels were changed in triploids following CPF exposures. In diploids, plasma T levels showed a linear dose-response reduction across CPF treatments correlating with liver weight and plasma total cholesterol concentrations. In contrast, no changes in plasma cholesterol and T concentrations were observed in triploids. Plasma cortisol and 17-β estradiol (E2) showed no response to CPF exposure in either ploidy. Results of this first comparison of biomarker responses to pesticide exposure in diploid and polyploid animals showed substantial differences between diploid and triploid C. gariepinus. |
| first_indexed | 2024-03-06T09:23:42Z |
| format | Article |
| id | upm.eprints-55532 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:23:42Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-555322017-09-13T02:12:03Z http://psasir.upm.edu.my/id/eprint/55532/ Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos Karami, Ali Goh, Yong Meng Jahromi, Mohammad Faseleh James Lazorchak, Ali Abdullah, Maha Courtenay, Simon C. The impacts of environmental stressors on polyploid organisms are largely unknown. This study investigated changes in morphometric, molecular, and biochemical parameters in full-sibling diploid and triploid African catfish (Clarias gariepinus) in response to chlorpyrifos (CPF) exposures. Juvenile fish were exposed to three concentrations of CPF (mean measured μg/L (SD): 9.71 (2.27), 15.7 (3.69), 31.21 (5.04)) under a static-renewal condition for 21 days. Diploid control groups had higher hepatosomatic index (HSI), plasma testosterone (T), and brain GnRH and cyp19a2 expression levels than triploids. In CPF-exposed groups, changes in HSI, total weight and length were different between the diploid and triploid fish. In contrast, condition factor did not alter in any of the treatments, while visceral-somatic index (VSI) changed only in diploids. In diploid fish, exposure to CPF did not change brain 11β-hsd2, ftz-f1, foxl2, GnRH or cyp19a2 mRNA levels, while reduced tph2 transcript levels compared to the control group. In contrast, 11β-hsd2 and foxl2 expression levels were changed in triploids following CPF exposures. In diploids, plasma T levels showed a linear dose-response reduction across CPF treatments correlating with liver weight and plasma total cholesterol concentrations. In contrast, no changes in plasma cholesterol and T concentrations were observed in triploids. Plasma cortisol and 17-β estradiol (E2) showed no response to CPF exposure in either ploidy. Results of this first comparison of biomarker responses to pesticide exposure in diploid and polyploid animals showed substantial differences between diploid and triploid C. gariepinus. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55532/1/Diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20differ%20in%20biomarker%20responses%20.pdf Karami, Ali and Goh, Yong Meng and Jahromi, Mohammad Faseleh and James Lazorchak, Ali and Abdullah, Maha and Courtenay, Simon C. (2016) Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos. Science of The Total Environment, 557558 (July). pp. 204-211. ISSN 0048-9697; ESSN: 1879-1026 10.1016/j.scitotenv.2016.03.030 |
| spellingShingle | Karami, Ali Goh, Yong Meng Jahromi, Mohammad Faseleh James Lazorchak, Ali Abdullah, Maha Courtenay, Simon C. Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos |
| title | Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos |
| title_full | Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos |
| title_fullStr | Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos |
| title_full_unstemmed | Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos |
| title_short | Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos |
| title_sort | diploid and triploid african catfish clarias gariepinus differ in biomarker responses to the pesticide chlorpyrifos |
| url | http://psasir.upm.edu.my/id/eprint/55532/1/Diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20differ%20in%20biomarker%20responses%20.pdf |
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