Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage
The nucleocapsids of hepatitis B virus (HBV) are made of 180 or 240 subunits of core proteins or known as core antigens (HBcAg). A fusion bacteriophage bearing the WSFFSNI sequence that interacts tightly to HBcAg was employed as a diagnostic reagent for the detection of the antigen using the phage-e...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5605/1/5605.pdf |
| _version_ | 1825943893523300352 |
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| author | Hasmoni, Siti Salwa Yusoff, Khatijah Tan, Wen Siang |
| author_facet | Hasmoni, Siti Salwa Yusoff, Khatijah Tan, Wen Siang |
| author_sort | Hasmoni, Siti Salwa |
| collection | UPM |
| description | The nucleocapsids of hepatitis B virus (HBV) are made of 180 or 240 subunits of core proteins or known as core antigens (HBcAg). A fusion bacteriophage bearing the WSFFSNI sequence that interacts tightly to HBcAg was employed as a diagnostic reagent for the detection of the antigen using the phage-enzyme-linked immunosorbent (phage-ELISA), dot blot and immunoprecipitation assays. The results from phage-ELISA and dot blot assay showed that as low as 10 ng of HBcAg can be detected optimally by 1.0×1012 pfu/ml fusion M13 bacteriophage. The sensitivity of the dot blot assay corresponds with that of the phage-ELISA. HBcAg in HBV positive serum samples can also be detected using the fusion phage via the phage-ELISA and phage-dot blot assay. The phage cross-linked to cyanogen bromide (CNBr) activated agarose can also be used to precipitate HBcAg in bacterial lysate. The optimum amount of phage needed for cross-linking to 1 g of agarose is about 7.0×106 pfu/ml which could also precipitate purified and unpurified HBcAg in bacterial lysate. This study demonstrates the potential of fusion bacteriophage bearing the sequence WSFFSNI as a diagnostic reagent and a ligand for the detection and purification of HBcAg respectively. |
| first_indexed | 2024-03-06T07:07:24Z |
| format | Article |
| id | upm.eprints-5605 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:07:24Z |
| publishDate | 2005 |
| publisher | Applied Microbiology, Molecular and Cellular Biosciences Research Foundation |
| record_format | dspace |
| spelling | upm.eprints-56052016-04-25T04:35:09Z http://psasir.upm.edu.my/id/eprint/5605/ Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage Hasmoni, Siti Salwa Yusoff, Khatijah Tan, Wen Siang The nucleocapsids of hepatitis B virus (HBV) are made of 180 or 240 subunits of core proteins or known as core antigens (HBcAg). A fusion bacteriophage bearing the WSFFSNI sequence that interacts tightly to HBcAg was employed as a diagnostic reagent for the detection of the antigen using the phage-enzyme-linked immunosorbent (phage-ELISA), dot blot and immunoprecipitation assays. The results from phage-ELISA and dot blot assay showed that as low as 10 ng of HBcAg can be detected optimally by 1.0×1012 pfu/ml fusion M13 bacteriophage. The sensitivity of the dot blot assay corresponds with that of the phage-ELISA. HBcAg in HBV positive serum samples can also be detected using the fusion phage via the phage-ELISA and phage-dot blot assay. The phage cross-linked to cyanogen bromide (CNBr) activated agarose can also be used to precipitate HBcAg in bacterial lysate. The optimum amount of phage needed for cross-linking to 1 g of agarose is about 7.0×106 pfu/ml which could also precipitate purified and unpurified HBcAg in bacterial lysate. This study demonstrates the potential of fusion bacteriophage bearing the sequence WSFFSNI as a diagnostic reagent and a ligand for the detection and purification of HBcAg respectively. Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5605/1/5605.pdf Hasmoni, Siti Salwa and Yusoff, Khatijah and Tan, Wen Siang (2005) Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage. Journal of General and Applied Microbiology, 51 (2). pp. 125-131. ISSN 0022-1260; ESSN: 1349-8037 https://www.jstage.jst.go.jp/article/jgam/51/2/51_2_125/_article 10.2323/jgam.51.125 |
| spellingShingle | Hasmoni, Siti Salwa Yusoff, Khatijah Tan, Wen Siang Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage |
| title | Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage |
| title_full | Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage |
| title_fullStr | Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage |
| title_full_unstemmed | Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage |
| title_short | Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage |
| title_sort | detection and precipitation of hepatitis b core antigen using a fusion bacteriophage |
| url | http://psasir.upm.edu.my/id/eprint/5605/1/5605.pdf |
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