Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology
An optimisation study of the large-scale enzymatic synthesis of a liquid wax ester from oleic acid and oleyl alcohol using Novozym 435 was carried out. Investigations were performed in batch mode with a stirred tank reactor (STR) with one multi-bladed impeller. Response surface methodology (RSM) bas...
| 主要な著者: | , , , , , , |
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| フォーマット: | 論文 |
| 言語: | English |
| 出版事項: |
Society of Chemical Industry
2006
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| オンライン･アクセス: | http://psasir.upm.edu.my/id/eprint/5606/1/Optimisation%20study%20of%20large.pdf |
| _version_ | 1825943893714141184 |
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| author | Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha |
| author_facet | Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha |
| author_sort | Mat Radzi, Salina |
| collection | UPM |
| description | An optimisation study of the large-scale enzymatic synthesis of a liquid wax ester from oleic acid and oleyl alcohol using Novozym 435 was carried out. Investigations were performed in batch mode with a stirred tank reactor (STR) with one multi-bladed impeller. Response surface methodology (RSM) based on a five-level, three-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of various parameters. The parameters are amount of enzyme (A) (90–120 g), impeller speed (B) (100–400 rpm) and temperature (C) (40–60 °C). The optimum conditions derived via RSM at a fixed reaction time of 1 h were successfully optimised as A = 104 g, B = 388.0 rpm and C = 49.7 °C. The actual experimental yield was 96.7% under the optimum conditions, which compared well with the maximum predicted value of 97.6%. |
| first_indexed | 2024-03-06T07:07:24Z |
| format | Article |
| id | upm.eprints-5606 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:07:24Z |
| publishDate | 2006 |
| publisher | Society of Chemical Industry |
| record_format | dspace |
| spelling | upm.eprints-56062016-09-28T03:23:24Z http://psasir.upm.edu.my/id/eprint/5606/ Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha An optimisation study of the large-scale enzymatic synthesis of a liquid wax ester from oleic acid and oleyl alcohol using Novozym 435 was carried out. Investigations were performed in batch mode with a stirred tank reactor (STR) with one multi-bladed impeller. Response surface methodology (RSM) based on a five-level, three-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of various parameters. The parameters are amount of enzyme (A) (90–120 g), impeller speed (B) (100–400 rpm) and temperature (C) (40–60 °C). The optimum conditions derived via RSM at a fixed reaction time of 1 h were successfully optimised as A = 104 g, B = 388.0 rpm and C = 49.7 °C. The actual experimental yield was 96.7% under the optimum conditions, which compared well with the maximum predicted value of 97.6%. Society of Chemical Industry 2006-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5606/1/Optimisation%20study%20of%20large.pdf Mat Radzi, Salina and Basri, Mahiran and Salleh, Abu Bakar and Ariff, Arbakariya and Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2006) Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology. Journal of Chemical Technology and Biotechnology, 81 (3). pp. 374-380. ISSN 0268-2575; ESSN: 1097-4660 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jctb.1404/abstract 10.1002/jctb.1404 |
| spellingShingle | Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology |
| title | Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology |
| title_full | Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology |
| title_fullStr | Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology |
| title_full_unstemmed | Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology |
| title_short | Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology |
| title_sort | optimisation study of large scale enzymatic synthesis of oleyl oleate a liquid wax ester by response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/5606/1/Optimisation%20study%20of%20large.pdf |
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