Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis
Surveys were conducted between the years of 2005 and 2006 at several locations in the northern, central and southern parts of West Malaysia to study the polymorphism of Ralstonia solanacearum strains. These sites included vegetables and farms with known hosts of the pathogen, such as banana, tomato,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5610/1/Study%20on%20intraspecific%20diversity%20of%20Ralstonia%20solanacearum%20strains%20in%20West%20Malaysia%20using%20whole%20cell%20fatty%20acid%20analysis.pdf |
| _version_ | 1825943894489038848 |
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| author | Khakvar, Reza Sijam, Kamaruzaman Thong, Kwai Lin Radu, Son Wong, Mui Yun Jones, Jeffery |
| author_facet | Khakvar, Reza Sijam, Kamaruzaman Thong, Kwai Lin Radu, Son Wong, Mui Yun Jones, Jeffery |
| author_sort | Khakvar, Reza |
| collection | UPM |
| description | Surveys were conducted between the years of 2005 and 2006 at several locations in the northern, central and southern parts of West Malaysia to study the polymorphism of Ralstonia solanacearum strains. These sites included vegetables and farms with known hosts of the pathogen, such as banana, tomato, eggplant, chili and tobacco. Samples were collected from the suspected wilted plants and weeds, including soil and water samples, in selected areas. The bacterium was isolated in all samples using semi-selective tetrazolium chloride medium (TZC). The bacteria strains were detected by using the BIOLOG identification system and were confirmed by nested-PCR. Fatty Acid Methyl Esters (FAME) profiling was performed to determine polymorphism among 58 bacterial isolates. The results showed that the fatty acid composition varied for all R. solanacearum isolates. Grouping of R. solanacearum isolates by fatty acid composition suggested that the existence of distinct groups that were significantly related to host of bacteria but low correlation between fatty acid profiles and biovar or sampling site was detected. A unique FAME profile was found among the strains that have been isolated from banana. |
| first_indexed | 2024-03-06T07:07:25Z |
| format | Article |
| id | upm.eprints-5610 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:07:25Z |
| publishDate | 2009 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-56102016-04-22T02:09:01Z http://psasir.upm.edu.my/id/eprint/5610/ Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis Khakvar, Reza Sijam, Kamaruzaman Thong, Kwai Lin Radu, Son Wong, Mui Yun Jones, Jeffery Surveys were conducted between the years of 2005 and 2006 at several locations in the northern, central and southern parts of West Malaysia to study the polymorphism of Ralstonia solanacearum strains. These sites included vegetables and farms with known hosts of the pathogen, such as banana, tomato, eggplant, chili and tobacco. Samples were collected from the suspected wilted plants and weeds, including soil and water samples, in selected areas. The bacterium was isolated in all samples using semi-selective tetrazolium chloride medium (TZC). The bacteria strains were detected by using the BIOLOG identification system and were confirmed by nested-PCR. Fatty Acid Methyl Esters (FAME) profiling was performed to determine polymorphism among 58 bacterial isolates. The results showed that the fatty acid composition varied for all R. solanacearum isolates. Grouping of R. solanacearum isolates by fatty acid composition suggested that the existence of distinct groups that were significantly related to host of bacteria but low correlation between fatty acid profiles and biovar or sampling site was detected. A unique FAME profile was found among the strains that have been isolated from banana. Taylor & Francis 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5610/1/Study%20on%20intraspecific%20diversity%20of%20Ralstonia%20solanacearum%20strains%20in%20West%20Malaysia%20using%20whole%20cell%20fatty%20acid%20analysis.pdf Khakvar, Reza and Sijam, Kamaruzaman and Thong, Kwai Lin and Radu, Son and Wong, Mui Yun and Jones, Jeffery (2009) Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis. Archives of Phytopathology and Plant Protection, 42 (6). pp. 594-601. ISSN 0323-5408; ESSN: 1477-2906 http://www.tandfonline.com/doi/abs/10.1080/03235400802478910 10.1080/03235400802478910 |
| spellingShingle | Khakvar, Reza Sijam, Kamaruzaman Thong, Kwai Lin Radu, Son Wong, Mui Yun Jones, Jeffery Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis |
| title | Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis |
| title_full | Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis |
| title_fullStr | Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis |
| title_full_unstemmed | Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis |
| title_short | Study on intraspecific diversity of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis |
| title_sort | study on intraspecific diversity of ralstonia solanacearum strains in west malaysia using whole cell fatty acid analysis |
| url | http://psasir.upm.edu.my/id/eprint/5610/1/Study%20on%20intraspecific%20diversity%20of%20Ralstonia%20solanacearum%20strains%20in%20West%20Malaysia%20using%20whole%20cell%20fatty%20acid%20analysis.pdf |
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