The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue
Previous studies carried out in mouse 3T3-L1 cell culture have shown that conjugated linoleic acid (CLA) inhibited adipocyte differentiation. The present study was undertaken to investigate the effect of cis-9, trans-11 and trans-10, cis-12 CLA isomers on morphological changes and on mRNA expression...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56206/1/The%20effects%20of%20conjugated%20linoleic%20acid%20isomers%20on%20the%20morphological%20changes%20in%20adipose%20tissue%20and%20adipogenic%20genes%20expressions%20on%20primary%20adipose%20tissue.pdf |
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| author | Ramiah, Suriya Kumari Goh, Yong Meng Yeap, Swee Keong Ebrahimi, Mahdi Tan, Sheau Wei |
| author_facet | Ramiah, Suriya Kumari Goh, Yong Meng Yeap, Swee Keong Ebrahimi, Mahdi Tan, Sheau Wei |
| author_sort | Ramiah, Suriya Kumari |
| collection | UPM |
| description | Previous studies carried out in mouse 3T3-L1 cell culture have shown that conjugated linoleic acid (CLA) inhibited adipocyte differentiation. The present study was undertaken to investigate the effect of cis-9, trans-11 and trans-10, cis-12 CLA isomers on morphological changes and on mRNA expressions in the in vitro adipocyte isolated from specific pathogen-free (SPF) chicken. The adipocytes were isolated from SPF chicken and cultured in differentiation-induction medium with different concentrations of cis-9, trans-11 and trans-10, cis-12 CLA. After day 7, the adipocyte differentiations were monitored morphologically and mRNA expressions of lipoprotein lipase (LPL) and acyl-coenzyme A binding domain containing 5 (ACBD 5) were quantified by real-time polymerase chain reaction (PCR) analysis. Our data suggested that cis-9, trans-11 CLA downregulated the expression of LPL and ACBD 5 genes, which was concurrently observed with decrease in the adipocyte area size and cell number compared to the control and trans-10, cis-12 treated groups. Based on this finding, we concluded that dietary CLA modulate fat reduction in chicken via alteration of transcription of key adipogenic genes and adipose cellularity. |
| first_indexed | 2024-03-06T09:25:42Z |
| format | Article |
| id | upm.eprints-56206 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:25:42Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-562062017-07-04T02:46:18Z http://psasir.upm.edu.my/id/eprint/56206/ The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue Ramiah, Suriya Kumari Goh, Yong Meng Yeap, Swee Keong Ebrahimi, Mahdi Tan, Sheau Wei Previous studies carried out in mouse 3T3-L1 cell culture have shown that conjugated linoleic acid (CLA) inhibited adipocyte differentiation. The present study was undertaken to investigate the effect of cis-9, trans-11 and trans-10, cis-12 CLA isomers on morphological changes and on mRNA expressions in the in vitro adipocyte isolated from specific pathogen-free (SPF) chicken. The adipocytes were isolated from SPF chicken and cultured in differentiation-induction medium with different concentrations of cis-9, trans-11 and trans-10, cis-12 CLA. After day 7, the adipocyte differentiations were monitored morphologically and mRNA expressions of lipoprotein lipase (LPL) and acyl-coenzyme A binding domain containing 5 (ACBD 5) were quantified by real-time polymerase chain reaction (PCR) analysis. Our data suggested that cis-9, trans-11 CLA downregulated the expression of LPL and ACBD 5 genes, which was concurrently observed with decrease in the adipocyte area size and cell number compared to the control and trans-10, cis-12 treated groups. Based on this finding, we concluded that dietary CLA modulate fat reduction in chicken via alteration of transcription of key adipogenic genes and adipose cellularity. Taylor & Francis 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56206/1/The%20effects%20of%20conjugated%20linoleic%20acid%20isomers%20on%20the%20morphological%20changes%20in%20adipose%20tissue%20and%20adipogenic%20genes%20expressions%20on%20primary%20adipose%20tissue.pdf Ramiah, Suriya Kumari and Goh, Yong Meng and Yeap, Swee Keong and Ebrahimi, Mahdi and Tan, Sheau Wei (2017) The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue. Italian Journal of Animal Science, 16 (2). pp. 253-258. ISSN 1828-051X http://www.tandfonline.com/doi/full/10.1080/1828051X.2016.1277961 10.1080/1828051X.2016.1277961 |
| spellingShingle | Ramiah, Suriya Kumari Goh, Yong Meng Yeap, Swee Keong Ebrahimi, Mahdi Tan, Sheau Wei The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue |
| title | The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue |
| title_full | The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue |
| title_fullStr | The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue |
| title_full_unstemmed | The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue |
| title_short | The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue |
| title_sort | effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue |
| url | http://psasir.upm.edu.my/id/eprint/56206/1/The%20effects%20of%20conjugated%20linoleic%20acid%20isomers%20on%20the%20morphological%20changes%20in%20adipose%20tissue%20and%20adipogenic%20genes%20expressions%20on%20primary%20adipose%20tissue.pdf |
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