Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat
Brucellosis, caused by Brucella melitensis, is a significant problem for both public and animal health worldwide. The Rose Bengal plate test (RBPT) antigen from Brucella melitensis local isolates were developed in this study. The performance of the assay was investigated using serum samples collecte...
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| Format: | Article |
| Language: | English |
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Department of Veterinary Services Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56573/1/Development%20of%20an%20in-house%20Rose%20Bengal%20plate%20test%20for%20diagnosis%20of%20brucellosis%20in%20goat.pdf |
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| author | Yahaya, Sani Mohammed Bejo, Siti Khairani Zakaria, Zunita Omar, Mohamed Ariff Bitrus, Asinamai Athliamai |
| author_facet | Yahaya, Sani Mohammed Bejo, Siti Khairani Zakaria, Zunita Omar, Mohamed Ariff Bitrus, Asinamai Athliamai |
| author_sort | Yahaya, Sani Mohammed |
| collection | UPM |
| description | Brucellosis, caused by Brucella melitensis, is a significant problem for both public and animal health worldwide. The Rose Bengal plate test (RBPT) antigen from Brucella melitensis local isolates were developed in this study. The performance of the assay was investigated using serum samples collected from goats. A total of 1063 serum samples obtained from goats were examined for the presence of antibodies against Brucella by in-house RBPT (LRBPT), commercial RBPT (Veterinary Laboratory Agency – VLA, UK) and Compliment Fixation test (CFT). The sensitivity and specificity was calculated using CFT as the gold standard. Out of 1063 goats sera analyzed 364 (34.24%), 335 (31.51%), and 373 (35.08%) were found to be positive by LRBPT, commercial RBPT and CFT, respectively. The sensitivity calculated for the LRBPT, was 90.1% compared to commercial RBPT 85.0%. However, the specificity of the LRBPT was lower (95.9%), than the commercial RBPT (97.4%). Furthermore the LRBPT has better value of NPV (94.7%) than commercial RBPT NPV (92.3%). While the PPV, of the commercial RBPT is higher (94.6%) than LRBPT (92.3%) respectively. High sensitive and low cost LRBPT compared to cRBPT B. melitensis RBPT test was successfully developed in this present study. Therefore it was concluded that this diagnostic test kit can complement and replace the available commercial RBPT which is relatively more expensive and less sensitive in detection of brucellosis in goats. It could also be used for epidemiological surveillance of caprine brucellosis in Malaysia. |
| first_indexed | 2024-03-06T09:26:48Z |
| format | Article |
| id | upm.eprints-56573 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:26:48Z |
| publishDate | 2016 |
| publisher | Department of Veterinary Services Malaysia |
| record_format | dspace |
| spelling | upm.eprints-565732017-08-03T04:53:10Z http://psasir.upm.edu.my/id/eprint/56573/ Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat Yahaya, Sani Mohammed Bejo, Siti Khairani Zakaria, Zunita Omar, Mohamed Ariff Bitrus, Asinamai Athliamai Brucellosis, caused by Brucella melitensis, is a significant problem for both public and animal health worldwide. The Rose Bengal plate test (RBPT) antigen from Brucella melitensis local isolates were developed in this study. The performance of the assay was investigated using serum samples collected from goats. A total of 1063 serum samples obtained from goats were examined for the presence of antibodies against Brucella by in-house RBPT (LRBPT), commercial RBPT (Veterinary Laboratory Agency – VLA, UK) and Compliment Fixation test (CFT). The sensitivity and specificity was calculated using CFT as the gold standard. Out of 1063 goats sera analyzed 364 (34.24%), 335 (31.51%), and 373 (35.08%) were found to be positive by LRBPT, commercial RBPT and CFT, respectively. The sensitivity calculated for the LRBPT, was 90.1% compared to commercial RBPT 85.0%. However, the specificity of the LRBPT was lower (95.9%), than the commercial RBPT (97.4%). Furthermore the LRBPT has better value of NPV (94.7%) than commercial RBPT NPV (92.3%). While the PPV, of the commercial RBPT is higher (94.6%) than LRBPT (92.3%) respectively. High sensitive and low cost LRBPT compared to cRBPT B. melitensis RBPT test was successfully developed in this present study. Therefore it was concluded that this diagnostic test kit can complement and replace the available commercial RBPT which is relatively more expensive and less sensitive in detection of brucellosis in goats. It could also be used for epidemiological surveillance of caprine brucellosis in Malaysia. Department of Veterinary Services Malaysia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56573/1/Development%20of%20an%20in-house%20Rose%20Bengal%20plate%20test%20for%20diagnosis%20of%20brucellosis%20in%20goat.pdf Yahaya, Sani Mohammed and Bejo, Siti Khairani and Zakaria, Zunita and Omar, Mohamed Ariff and Bitrus, Asinamai Athliamai (2016) Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat. Malaysian Journal of Veterinary Research, 7 (2). pp. 59-65. ISSN 2180-3897 http://www.dvs.gov.my/index.php/pages/view/1592 |
| spellingShingle | Yahaya, Sani Mohammed Bejo, Siti Khairani Zakaria, Zunita Omar, Mohamed Ariff Bitrus, Asinamai Athliamai Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat |
| title | Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat |
| title_full | Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat |
| title_fullStr | Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat |
| title_full_unstemmed | Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat |
| title_short | Development of an in-house Rose Bengal plate test for diagnosis of brucellosis in goat |
| title_sort | development of an in house rose bengal plate test for diagnosis of brucellosis in goat |
| url | http://psasir.upm.edu.my/id/eprint/56573/1/Development%20of%20an%20in-house%20Rose%20Bengal%20plate%20test%20for%20diagnosis%20of%20brucellosis%20in%20goat.pdf |
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