Staphylococcus aureus carriage in selected kindergartens in Klang Valley
Introduction: Nasal colonisation of S. aureus in healthy children was 18% to 30%. One to three percent of them were colonised by Methicillin-resistant Staphlycoccus aureus (MRSA). Although MRSA infection has become increasingly reported, population-based S. aureus and MRSA colonisation estimates are...
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| Format: | Article |
| Language: | English |
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Malaysian Medical Association
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56861/1/Staphylococcus%20aureus%20carriage%20in%20selected%20kindergartens%20in%20Klang%20Valley.pdf |
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| author | Mohamed @ Abd Aziz, Nurul Azmawati Ramli, Shalinawati Zulkifli Amin, Nur Natasha Wan Sulaiman, Wan Shahida Che Isahak, Che Ilina Tengku Jamaluddin, Tengku Zetty Maztura Mohammed Salleh, Nooriah |
| author_facet | Mohamed @ Abd Aziz, Nurul Azmawati Ramli, Shalinawati Zulkifli Amin, Nur Natasha Wan Sulaiman, Wan Shahida Che Isahak, Che Ilina Tengku Jamaluddin, Tengku Zetty Maztura Mohammed Salleh, Nooriah |
| author_sort | Mohamed @ Abd Aziz, Nurul Azmawati |
| collection | UPM |
| description | Introduction: Nasal colonisation of S. aureus in healthy children was 18% to 30%. One to three percent of them were colonised by Methicillin-resistant Staphlycoccus aureus (MRSA). Although MRSA infection has become increasingly reported, population-based S. aureus and MRSA colonisation estimates are lacking. The main objective of this study was to determine the prevalence of S. aureus carriage among children.
Methods: Nasal samples for S. aureus culture were obtained from 250 children from three kindergartens in the Klang Valley, after consent was obtained from the children and their parents. Swabs were transported in Stuart medium, and inoculated on mannitol-salt agar within four hours of collection. Identification and disk diffusion test were done according to guidelines. Polymerase chain reaction was done on MRSA isolates for the presence of mecA and lukS/FPV genes.
Results: Overall prevalence of S. aureus and MRSA carriage were 19.2% (48/250) and 1.6% (4/250) respectively. mecA gene was present in all isolates, 50% isolates carried Panton-Valentine leucocidin (PVL) gene. Sccmec type I was found in 2 isolates and the remaining isolates has Sccmec type V.
Conclusion: The prevalence of S. aureus and MRSA carriage were similar to other studies. However, risk of contracting severe infection might be higher due to presence of PVL gene in half of the MRSA isolates. |
| first_indexed | 2024-03-06T09:27:40Z |
| format | Article |
| id | upm.eprints-56861 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:27:40Z |
| publishDate | 2016 |
| publisher | Malaysian Medical Association |
| record_format | dspace |
| spelling | upm.eprints-568612019-10-03T02:26:26Z http://psasir.upm.edu.my/id/eprint/56861/ Staphylococcus aureus carriage in selected kindergartens in Klang Valley Mohamed @ Abd Aziz, Nurul Azmawati Ramli, Shalinawati Zulkifli Amin, Nur Natasha Wan Sulaiman, Wan Shahida Che Isahak, Che Ilina Tengku Jamaluddin, Tengku Zetty Maztura Mohammed Salleh, Nooriah Introduction: Nasal colonisation of S. aureus in healthy children was 18% to 30%. One to three percent of them were colonised by Methicillin-resistant Staphlycoccus aureus (MRSA). Although MRSA infection has become increasingly reported, population-based S. aureus and MRSA colonisation estimates are lacking. The main objective of this study was to determine the prevalence of S. aureus carriage among children. Methods: Nasal samples for S. aureus culture were obtained from 250 children from three kindergartens in the Klang Valley, after consent was obtained from the children and their parents. Swabs were transported in Stuart medium, and inoculated on mannitol-salt agar within four hours of collection. Identification and disk diffusion test were done according to guidelines. Polymerase chain reaction was done on MRSA isolates for the presence of mecA and lukS/FPV genes. Results: Overall prevalence of S. aureus and MRSA carriage were 19.2% (48/250) and 1.6% (4/250) respectively. mecA gene was present in all isolates, 50% isolates carried Panton-Valentine leucocidin (PVL) gene. Sccmec type I was found in 2 isolates and the remaining isolates has Sccmec type V. Conclusion: The prevalence of S. aureus and MRSA carriage were similar to other studies. However, risk of contracting severe infection might be higher due to presence of PVL gene in half of the MRSA isolates. Malaysian Medical Association 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56861/1/Staphylococcus%20aureus%20carriage%20in%20selected%20kindergartens%20in%20Klang%20Valley.pdf Mohamed @ Abd Aziz, Nurul Azmawati and Ramli, Shalinawati and Zulkifli Amin, Nur Natasha and Wan Sulaiman, Wan Shahida and Che Isahak, Che Ilina and Tengku Jamaluddin, Tengku Zetty Maztura and Mohammed Salleh, Nooriah (2016) Staphylococcus aureus carriage in selected kindergartens in Klang Valley. Medical Journal of Malaysia, 71 (2). pp. 62-65. ISSN 0300-5283 http://www.e-mjm.org/2016/v71n2/index.html |
| spellingShingle | Mohamed @ Abd Aziz, Nurul Azmawati Ramli, Shalinawati Zulkifli Amin, Nur Natasha Wan Sulaiman, Wan Shahida Che Isahak, Che Ilina Tengku Jamaluddin, Tengku Zetty Maztura Mohammed Salleh, Nooriah Staphylococcus aureus carriage in selected kindergartens in Klang Valley |
| title | Staphylococcus aureus carriage in selected kindergartens in Klang Valley |
| title_full | Staphylococcus aureus carriage in selected kindergartens in Klang Valley |
| title_fullStr | Staphylococcus aureus carriage in selected kindergartens in Klang Valley |
| title_full_unstemmed | Staphylococcus aureus carriage in selected kindergartens in Klang Valley |
| title_short | Staphylococcus aureus carriage in selected kindergartens in Klang Valley |
| title_sort | staphylococcus aureus carriage in selected kindergartens in klang valley |
| url | http://psasir.upm.edu.my/id/eprint/56861/1/Staphylococcus%20aureus%20carriage%20in%20selected%20kindergartens%20in%20Klang%20Valley.pdf |
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