Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa)
Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high leve...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Science Publications
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/57076/1/ajisp.2005.31.36.pdf |
| _version_ | 1825931540402536448 |
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| author | Bilung, Lesley Maurice Radu, Son Bahaman, Abdul Rani Abdul Rahim, Raha Napis, Suhaimi Cheah, Yoke Kqueen Murugaiah, Chandrika Abdul Hadi, Yousr Robin, Tunung Nishibuchi, Mitsuaki |
| author_facet | Bilung, Lesley Maurice Radu, Son Bahaman, Abdul Rani Abdul Rahim, Raha Napis, Suhaimi Cheah, Yoke Kqueen Murugaiah, Chandrika Abdul Hadi, Yousr Robin, Tunung Nishibuchi, Mitsuaki |
| author_sort | Bilung, Lesley Maurice |
| collection | UPM |
| description | Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains. |
| first_indexed | 2024-03-06T09:28:18Z |
| format | Article |
| id | upm.eprints-57076 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:28:18Z |
| publishDate | 2005 |
| publisher | Science Publications |
| record_format | dspace |
| spelling | upm.eprints-570762017-09-07T03:47:17Z http://psasir.upm.edu.my/id/eprint/57076/ Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa) Bilung, Lesley Maurice Radu, Son Bahaman, Abdul Rani Abdul Rahim, Raha Napis, Suhaimi Cheah, Yoke Kqueen Murugaiah, Chandrika Abdul Hadi, Yousr Robin, Tunung Nishibuchi, Mitsuaki Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains. Science Publications 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/57076/1/ajisp.2005.31.36.pdf Bilung, Lesley Maurice and Radu, Son and Bahaman, Abdul Rani and Abdul Rahim, Raha and Napis, Suhaimi and Cheah, Yoke Kqueen and Murugaiah, Chandrika and Abdul Hadi, Yousr and Robin, Tunung and Nishibuchi, Mitsuaki (2005) Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa). American Journal of Immunology, 1 (1). pp. 31-36. ISSN 1553-619X; ESSN: 1558-3775 http://thescipub.com/html/10.3844/ajisp.2005.31.36 10.3844/ajisp.2005.31.36 |
| spellingShingle | Bilung, Lesley Maurice Radu, Son Bahaman, Abdul Rani Abdul Rahim, Raha Napis, Suhaimi Cheah, Yoke Kqueen Murugaiah, Chandrika Abdul Hadi, Yousr Robin, Tunung Nishibuchi, Mitsuaki Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa) |
| title | Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa) |
| title_full | Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa) |
| title_fullStr | Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa) |
| title_full_unstemmed | Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa) |
| title_short | Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa) |
| title_sort | random amplified polymorphic dna pcr typing of vibrio parahaemolyticus isolated from local cockles anadara granosa |
| url | http://psasir.upm.edu.my/id/eprint/57076/1/ajisp.2005.31.36.pdf |
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