Biophysical properties of DNA in hydrated ionic liquids
The biophysical properties and behavior of natural calf thymus DNA in hydrated 1-ethyl-3-butylimidazolium bromide ionic liquid ([C2bim]Br) have been studied using spectroscopy technique. The effect of ionic liquid concentration and temperature towards the duplex B-DNA conformation were determined. T...
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| Format: | Conference or Workshop Item |
| Language: | English |
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AIP Publishing
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/57425/1/Biophysical%20properties%20of%20DNA%20in%20hydrated%20ionic%20liquids.pdf |
| _version_ | 1825931609969262592 |
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| author | Jumbri, Khairulazhar Ahmad, Haslina Abd. Malek, Emilia Abdul Rahman, Mohd Basyaruddin |
| author_facet | Jumbri, Khairulazhar Ahmad, Haslina Abd. Malek, Emilia Abdul Rahman, Mohd Basyaruddin |
| author_sort | Jumbri, Khairulazhar |
| collection | UPM |
| description | The biophysical properties and behavior of natural calf thymus DNA in hydrated 1-ethyl-3-butylimidazolium bromide ionic liquid ([C2bim]Br) have been studied using spectroscopy technique. The effect of ionic liquid concentration and temperature towards the duplex B-DNA conformation were determined. The presence of ionic liquid causes higher duplex DNA stability with the DNA melting temperature of ∼56°C without any addition of buffer solutions. The electrostatic attraction between ionic liquid’s cation and DNA phosphates groups was found play a main role in stabilizing native DNA structure. Understanding of the biophysical properties of DNA in this ionic media could be used as a platform for future development of specific solvent for nucleic acid nanotechnology. |
| first_indexed | 2024-03-06T09:29:21Z |
| format | Conference or Workshop Item |
| id | upm.eprints-57425 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:29:21Z |
| publishDate | 2016 |
| publisher | AIP Publishing |
| record_format | dspace |
| spelling | upm.eprints-574252017-09-27T10:13:22Z http://psasir.upm.edu.my/id/eprint/57425/ Biophysical properties of DNA in hydrated ionic liquids Jumbri, Khairulazhar Ahmad, Haslina Abd. Malek, Emilia Abdul Rahman, Mohd Basyaruddin The biophysical properties and behavior of natural calf thymus DNA in hydrated 1-ethyl-3-butylimidazolium bromide ionic liquid ([C2bim]Br) have been studied using spectroscopy technique. The effect of ionic liquid concentration and temperature towards the duplex B-DNA conformation were determined. The presence of ionic liquid causes higher duplex DNA stability with the DNA melting temperature of ∼56°C without any addition of buffer solutions. The electrostatic attraction between ionic liquid’s cation and DNA phosphates groups was found play a main role in stabilizing native DNA structure. Understanding of the biophysical properties of DNA in this ionic media could be used as a platform for future development of specific solvent for nucleic acid nanotechnology. AIP Publishing 2016 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/57425/1/Biophysical%20properties%20of%20DNA%20in%20hydrated%20ionic%20liquids.pdf Jumbri, Khairulazhar and Ahmad, Haslina and Abd. Malek, Emilia and Abdul Rahman, Mohd Basyaruddin (2016) Biophysical properties of DNA in hydrated ionic liquids. In: 4th International Conference of Fundamental and Applied Sciences (ICFAS 2016), 15-17 Aug. 2016, Kuala Lumpur Convention Centre, Kuala Lumpur. (pp. 1-7). 10.1063/1.4968137 |
| spellingShingle | Jumbri, Khairulazhar Ahmad, Haslina Abd. Malek, Emilia Abdul Rahman, Mohd Basyaruddin Biophysical properties of DNA in hydrated ionic liquids |
| title | Biophysical properties of DNA in hydrated ionic liquids |
| title_full | Biophysical properties of DNA in hydrated ionic liquids |
| title_fullStr | Biophysical properties of DNA in hydrated ionic liquids |
| title_full_unstemmed | Biophysical properties of DNA in hydrated ionic liquids |
| title_short | Biophysical properties of DNA in hydrated ionic liquids |
| title_sort | biophysical properties of dna in hydrated ionic liquids |
| url | http://psasir.upm.edu.my/id/eprint/57425/1/Biophysical%20properties%20of%20DNA%20in%20hydrated%20ionic%20liquids.pdf |
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