Genes associated to wounding from Aquilaria malaccensis
Agarwood is an aromatic oleoresin produced by Aquilaria in response to wounding and biological attack but the molecular mechanism is unknown. In this study, several candidate genes in wounding and defense responses were cloned and identified. Deduced amino acid of the cloned sequences exhibited sequ...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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Faculty of Forestry, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58007/1/31-5.pdf |
| _version_ | 1825931725643972608 |
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| author | Wong, Mun Theng Siah, Chai Har Qamaruz Zaman, Faridah Mohamed, Rozi |
| author_facet | Wong, Mun Theng Siah, Chai Har Qamaruz Zaman, Faridah Mohamed, Rozi |
| author_sort | Wong, Mun Theng |
| collection | UPM |
| description | Agarwood is an aromatic oleoresin produced by Aquilaria in response to wounding and biological attack but the molecular mechanism is unknown. In this study, several candidate genes in wounding and defense responses were cloned and identified. Deduced amino acid of the cloned sequences exhibited sequence similarities to their respective homologs: transcription factors of the WRKY gene family (AmWRKY) and β-1,3-glucanase (AmGLU). Phenylalanine ammonia-lyase (AmPAL) from previous work was also included. A wounding-stress experiment revealed their expression profiles via qRT-PCR analysis. AmWRKY was induced early after wounding (3 hours), while AmPAL and AmGLU expressions were induced later (16 hours). It is possible that AmWRKY mediates early wounding response while AmPAL mediates response to fungal infection by co-inducing AmGLU. |
| first_indexed | 2024-03-06T09:31:06Z |
| format | Conference or Workshop Item |
| id | upm.eprints-58007 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:31:06Z |
| publishDate | 2013 |
| publisher | Faculty of Forestry, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-580072018-01-03T04:49:28Z http://psasir.upm.edu.my/id/eprint/58007/ Genes associated to wounding from Aquilaria malaccensis Wong, Mun Theng Siah, Chai Har Qamaruz Zaman, Faridah Mohamed, Rozi Agarwood is an aromatic oleoresin produced by Aquilaria in response to wounding and biological attack but the molecular mechanism is unknown. In this study, several candidate genes in wounding and defense responses were cloned and identified. Deduced amino acid of the cloned sequences exhibited sequence similarities to their respective homologs: transcription factors of the WRKY gene family (AmWRKY) and β-1,3-glucanase (AmGLU). Phenylalanine ammonia-lyase (AmPAL) from previous work was also included. A wounding-stress experiment revealed their expression profiles via qRT-PCR analysis. AmWRKY was induced early after wounding (3 hours), while AmPAL and AmGLU expressions were induced later (16 hours). It is possible that AmWRKY mediates early wounding response while AmPAL mediates response to fungal infection by co-inducing AmGLU. Faculty of Forestry, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58007/1/31-5.pdf Wong, Mun Theng and Siah, Chai Har and Qamaruz Zaman, Faridah and Mohamed, Rozi (2013) Genes associated to wounding from Aquilaria malaccensis. In: International Forestry Graduate Students' Conference 2013 (FORGRAD 2013), 2-4 July 2013, Universiti Putra Malaysia, Serdang, Selangor. (pp. 27-31). |
| spellingShingle | Wong, Mun Theng Siah, Chai Har Qamaruz Zaman, Faridah Mohamed, Rozi Genes associated to wounding from Aquilaria malaccensis |
| title | Genes associated to wounding from Aquilaria malaccensis |
| title_full | Genes associated to wounding from Aquilaria malaccensis |
| title_fullStr | Genes associated to wounding from Aquilaria malaccensis |
| title_full_unstemmed | Genes associated to wounding from Aquilaria malaccensis |
| title_short | Genes associated to wounding from Aquilaria malaccensis |
| title_sort | genes associated to wounding from aquilaria malaccensis |
| url | http://psasir.upm.edu.my/id/eprint/58007/1/31-5.pdf |
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