The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract
The comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of intracellular enzyme, glyceraldehydes 3-phosphate dehydrogenase (G3PDH) from unclarified disrupted yeast has been undertaken. The commercially available anion exchanger, Streamline QXL and the kiesleguh...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Malaysian Society for Molecular Biology and Biotechnology
2005
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/58492/1/The%20comparative%20recovery%20performance%20of%20anion%20exchange%20and%20dye-ligand%20fluidised%20bed%20adsorption%20of%20G3PDH%20from%20unclarified%20yeast%20extract.pdf |
| _version_ | 1825931821097943040 |
|---|---|
| author | Ling, Tau Chuan Lyddiatt, Andrew |
| author_facet | Ling, Tau Chuan Lyddiatt, Andrew |
| author_sort | Ling, Tau Chuan |
| collection | UPM |
| description | The comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of intracellular enzyme, glyceraldehydes 3-phosphate dehydrogenase (G3PDH) from unclarified disrupted yeast has been undertaken. The commercially available anion exchanger, Streamline QXL and the kiesleguhr-agarose composite adsorbent, Microsorb K6AX derived with dye-ligand (Cibaron Blue 3GA) were employed in fluidized bed experiments. The adsorbents were evaluated in respect of recovery performance in terms of yield, purity and enzyme specific activity. |
| first_indexed | 2024-03-06T09:32:33Z |
| format | Article |
| id | upm.eprints-58492 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:32:33Z |
| publishDate | 2005 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | upm.eprints-584922018-01-18T09:54:09Z http://psasir.upm.edu.my/id/eprint/58492/ The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract Ling, Tau Chuan Lyddiatt, Andrew The comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of intracellular enzyme, glyceraldehydes 3-phosphate dehydrogenase (G3PDH) from unclarified disrupted yeast has been undertaken. The commercially available anion exchanger, Streamline QXL and the kiesleguhr-agarose composite adsorbent, Microsorb K6AX derived with dye-ligand (Cibaron Blue 3GA) were employed in fluidized bed experiments. The adsorbents were evaluated in respect of recovery performance in terms of yield, purity and enzyme specific activity. Malaysian Society for Molecular Biology and Biotechnology 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58492/1/The%20comparative%20recovery%20performance%20of%20anion%20exchange%20and%20dye-ligand%20fluidised%20bed%20adsorption%20of%20G3PDH%20from%20unclarified%20yeast%20extract.pdf Ling, Tau Chuan and Lyddiatt, Andrew (2005) The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract. Asia Pacific Journal of Molecular Biology and Biotechnology, 13 (1). pp. 59-62. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html13/13e.htm |
| spellingShingle | Ling, Tau Chuan Lyddiatt, Andrew The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract |
| title | The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract |
| title_full | The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract |
| title_fullStr | The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract |
| title_full_unstemmed | The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract |
| title_short | The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract |
| title_sort | comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of g3pdh from unclarified yeast extract |
| url | http://psasir.upm.edu.my/id/eprint/58492/1/The%20comparative%20recovery%20performance%20of%20anion%20exchange%20and%20dye-ligand%20fluidised%20bed%20adsorption%20of%20G3PDH%20from%20unclarified%20yeast%20extract.pdf |
| work_keys_str_mv | AT lingtauchuan thecomparativerecoveryperformanceofanionexchangeanddyeligandfluidisedbedadsorptionofg3pdhfromunclarifiedyeastextract AT lyddiattandrew thecomparativerecoveryperformanceofanionexchangeanddyeligandfluidisedbedadsorptionofg3pdhfromunclarifiedyeastextract AT lingtauchuan comparativerecoveryperformanceofanionexchangeanddyeligandfluidisedbedadsorptionofg3pdhfromunclarifiedyeastextract AT lyddiattandrew comparativerecoveryperformanceofanionexchangeanddyeligandfluidisedbedadsorptionofg3pdhfromunclarifiedyeastextract |