Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones
This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven...
| Những tác giả chính: | , , , , |
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| Định dạng: | Bài viết |
| Ngôn ngữ: | English |
| Được phát hành: |
Malaysian Society for Molecular Biology and Biotechnology
2007
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| Truy cập trực tuyến: | http://psasir.upm.edu.my/id/eprint/58512/1/Development%20of%20simple%20sequence%20repeat%20%28SSR%29%20markers%20for%20oil%20palm%20and%20their%20application%20in%20genetic%20mapping%20and%20fingerprinting%20of%20tissue%20culture%20clones.pdf |
| _version_ | 1825931824989208576 |
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| author | Singh, Rajinder Nagappan, Jayanthi Tan, Soon Guan Panandam, Jothi Malar Cheah, Suan Choo |
| author_facet | Singh, Rajinder Nagappan, Jayanthi Tan, Soon Guan Panandam, Jothi Malar Cheah, Suan Choo |
| author_sort | Singh, Rajinder |
| collection | UPM |
| description | This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven of the 12 SSR markers exhibited expected Mendelian segregation ratios when tested on a mapping population, indicating their suitability for genetic mapping studies. The markers identified in the species Elaeis oleifera also showed applicability in a second species, that is Elaeis guineensis. Apart from genetic mapping, the SSR markers also showed promise as molecular probes for DNA fingerprinting of oil palm tissue culture clones. The SSR markers can be used for clonal identification, monitoring line uniformity between and within clones and detecting culture mix-up. |
| first_indexed | 2024-03-06T09:32:36Z |
| format | Article |
| id | upm.eprints-58512 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:32:36Z |
| publishDate | 2007 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | upm.eprints-585122018-01-19T09:53:03Z http://psasir.upm.edu.my/id/eprint/58512/ Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones Singh, Rajinder Nagappan, Jayanthi Tan, Soon Guan Panandam, Jothi Malar Cheah, Suan Choo This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven of the 12 SSR markers exhibited expected Mendelian segregation ratios when tested on a mapping population, indicating their suitability for genetic mapping studies. The markers identified in the species Elaeis oleifera also showed applicability in a second species, that is Elaeis guineensis. Apart from genetic mapping, the SSR markers also showed promise as molecular probes for DNA fingerprinting of oil palm tissue culture clones. The SSR markers can be used for clonal identification, monitoring line uniformity between and within clones and detecting culture mix-up. Malaysian Society for Molecular Biology and Biotechnology 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58512/1/Development%20of%20simple%20sequence%20repeat%20%28SSR%29%20markers%20for%20oil%20palm%20and%20their%20application%20in%20genetic%20mapping%20and%20fingerprinting%20of%20tissue%20culture%20clones.pdf Singh, Rajinder and Nagappan, Jayanthi and Tan, Soon Guan and Panandam, Jothi Malar and Cheah, Suan Choo (2007) Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones. Asia Pacific Journal of Molecular Biology and Biotechnology, 15 (3). pp. 121-131. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html153/153d.htm |
| spellingShingle | Singh, Rajinder Nagappan, Jayanthi Tan, Soon Guan Panandam, Jothi Malar Cheah, Suan Choo Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones |
| title | Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones |
| title_full | Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones |
| title_fullStr | Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones |
| title_full_unstemmed | Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones |
| title_short | Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones |
| title_sort | development of simple sequence repeat ssr markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones |
| url | http://psasir.upm.edu.my/id/eprint/58512/1/Development%20of%20simple%20sequence%20repeat%20%28SSR%29%20markers%20for%20oil%20palm%20and%20their%20application%20in%20genetic%20mapping%20and%20fingerprinting%20of%20tissue%20culture%20clones.pdf |
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