Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu
Polyphenol oxidase (PPO) and peroxidase (POD) activities were visualized histochemically at the cellular level of a young and a mature tree of Metroxylon sagu employing histochemical technique. In the mature tree, intense PPO activity was observed in the cell wall of the parenchyma and xylem cells w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58519/1/Histochemical%20localization%20of%20polyphenol%20oxidase%20and%20peroxidase%20from%20Metroxylon%20sagu.pdf |
| _version_ | 1796976917012807680 |
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| author | Onsa, Galila Hassan Saari, Nazamid Selamat, Jinap Bakar, Jamilah Mohammed, Abdulkarim Sabo Abd Razak, Shamsul Bahri |
| author_facet | Onsa, Galila Hassan Saari, Nazamid Selamat, Jinap Bakar, Jamilah Mohammed, Abdulkarim Sabo Abd Razak, Shamsul Bahri |
| author_sort | Onsa, Galila Hassan |
| collection | UPM |
| description | Polyphenol oxidase (PPO) and peroxidase (POD) activities were visualized histochemically at the cellular level of a young and a mature tree of Metroxylon sagu employing histochemical technique. In the mature tree, intense PPO activity was observed in the cell wall of the parenchyma and xylem cells when visualized under light microscope. Pith collected from the young tree showed PPO activity in the amyloplast and mitochondria inner membrane and to some extent in the golgi complex and endoplasmic reticulum. Whereas, a positive POD reaction product was visualized in the cell wall, peroxisomes and to some extent in the cytoplasm and the vacuole. The localization of PPO activity in the amyloplast and being adsorbed by the starch granules is in line with the general view that enzyme is involved in the browning of sago starch. |
| first_indexed | 2024-03-06T09:32:38Z |
| format | Article |
| id | upm.eprints-58519 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:32:38Z |
| publishDate | 2007 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | upm.eprints-585192018-01-19T10:17:14Z http://psasir.upm.edu.my/id/eprint/58519/ Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu Onsa, Galila Hassan Saari, Nazamid Selamat, Jinap Bakar, Jamilah Mohammed, Abdulkarim Sabo Abd Razak, Shamsul Bahri Polyphenol oxidase (PPO) and peroxidase (POD) activities were visualized histochemically at the cellular level of a young and a mature tree of Metroxylon sagu employing histochemical technique. In the mature tree, intense PPO activity was observed in the cell wall of the parenchyma and xylem cells when visualized under light microscope. Pith collected from the young tree showed PPO activity in the amyloplast and mitochondria inner membrane and to some extent in the golgi complex and endoplasmic reticulum. Whereas, a positive POD reaction product was visualized in the cell wall, peroxisomes and to some extent in the cytoplasm and the vacuole. The localization of PPO activity in the amyloplast and being adsorbed by the starch granules is in line with the general view that enzyme is involved in the browning of sago starch. Malaysian Society for Molecular Biology and Biotechnology 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58519/1/Histochemical%20localization%20of%20polyphenol%20oxidase%20and%20peroxidase%20from%20Metroxylon%20sagu.pdf Onsa, Galila Hassan and Saari, Nazamid and Selamat, Jinap and Bakar, Jamilah and Mohammed, Abdulkarim Sabo and Abd Razak, Shamsul Bahri (2007) Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu. Asia Pacific Journal of Molecular Biology and Biotechnology, 15 (2). pp. 91-98. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html152/152f.htm |
| spellingShingle | Onsa, Galila Hassan Saari, Nazamid Selamat, Jinap Bakar, Jamilah Mohammed, Abdulkarim Sabo Abd Razak, Shamsul Bahri Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu |
| title | Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu |
| title_full | Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu |
| title_fullStr | Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu |
| title_full_unstemmed | Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu |
| title_short | Histochemical localization of polyphenol oxidase and peroxidase from Metroxylon sagu |
| title_sort | histochemical localization of polyphenol oxidase and peroxidase from metroxylon sagu |
| url | http://psasir.upm.edu.my/id/eprint/58519/1/Histochemical%20localization%20of%20polyphenol%20oxidase%20and%20peroxidase%20from%20Metroxylon%20sagu.pdf |
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