Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia
This study describes the isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia (Nile Tilapia Oreochromis niloticus × Mozambique Tilapia O. mossambicus) in Malaysia. The isolated gram-positive S. iniae appeared punctiform, transparently white, catalase and oxidase negative...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Fisheries Society
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/59615/1/Isolation%20and%20pathogenicity%20of%20Streptococcus%20iniae%20in%20cultured%20red%20hybrid%20tilapia%20in%20Malaysia.pdf |
| _version_ | 1825932034948726784 |
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| author | Rahmatullah, Muhammad Ariff, M. Kahieshesfandiari, M. Mohd Daud, Hassan Saad, Mohd Zamri Mohd Yusoff, Md Sabri Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany |
| author_facet | Rahmatullah, Muhammad Ariff, M. Kahieshesfandiari, M. Mohd Daud, Hassan Saad, Mohd Zamri Mohd Yusoff, Md Sabri Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany |
| author_sort | Rahmatullah, Muhammad |
| collection | UPM |
| description | This study describes the isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia (Nile Tilapia Oreochromis niloticus × Mozambique Tilapia O. mossambicus) in Malaysia. The isolated gram-positive S. iniae appeared punctiform, transparently white, catalase and oxidase negative and produced complete β-hemolysis on blood agar, while a PCR assay resulted in the amplification of the 16 S rRNA gene and lactate oxidase encoded genes. The isolate was sensitive to tetracycline, vancomycin, and bacitracin but was resistant to streptomycin, ampicillin, penicillin, and erythromycin. Pathogenicity trials conducted in local red hybrid tilapia (mean ± SE = 20.00 ± 0.45 g) showed 90.0, 96.7, and 100.0% mortality within 14 d postinfection following intraperitoneal exposure to 104, 106, and 108 CFU/mL of the pathogen, respectively. The clinical signs included erratic swimming, lethargy, and inappetance at 6 h postinfection, while mortality was recorded at less than 24 h postinfection in all infected groups. The LD50-336 h of S. iniae against the red hybrid tilapia was 102 CFU/mL. The post mortem examinations revealed congested livers, kidneys, and spleens of the infected fish. This is the first report of S. iniae experimental infection in cultured red hybrid tilapia in Malaysia. |
| first_indexed | 2024-03-06T09:35:48Z |
| format | Article |
| id | upm.eprints-59615 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:35:48Z |
| publishDate | 2017 |
| publisher | American Fisheries Society |
| record_format | dspace |
| spelling | upm.eprints-596152018-03-13T08:12:35Z http://psasir.upm.edu.my/id/eprint/59615/ Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia Rahmatullah, Muhammad Ariff, M. Kahieshesfandiari, M. Mohd Daud, Hassan Saad, Mohd Zamri Mohd Yusoff, Md Sabri Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany This study describes the isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia (Nile Tilapia Oreochromis niloticus × Mozambique Tilapia O. mossambicus) in Malaysia. The isolated gram-positive S. iniae appeared punctiform, transparently white, catalase and oxidase negative and produced complete β-hemolysis on blood agar, while a PCR assay resulted in the amplification of the 16 S rRNA gene and lactate oxidase encoded genes. The isolate was sensitive to tetracycline, vancomycin, and bacitracin but was resistant to streptomycin, ampicillin, penicillin, and erythromycin. Pathogenicity trials conducted in local red hybrid tilapia (mean ± SE = 20.00 ± 0.45 g) showed 90.0, 96.7, and 100.0% mortality within 14 d postinfection following intraperitoneal exposure to 104, 106, and 108 CFU/mL of the pathogen, respectively. The clinical signs included erratic swimming, lethargy, and inappetance at 6 h postinfection, while mortality was recorded at less than 24 h postinfection in all infected groups. The LD50-336 h of S. iniae against the red hybrid tilapia was 102 CFU/mL. The post mortem examinations revealed congested livers, kidneys, and spleens of the infected fish. This is the first report of S. iniae experimental infection in cultured red hybrid tilapia in Malaysia. American Fisheries Society 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59615/1/Isolation%20and%20pathogenicity%20of%20Streptococcus%20iniae%20in%20cultured%20red%20hybrid%20tilapia%20in%20Malaysia.pdf Rahmatullah, Muhammad and Ariff, M. and Kahieshesfandiari, M. and Mohd Daud, Hassan and Saad, Mohd Zamri and Mohd Yusoff, Md Sabri and Azmai, Mohammad Noor Amal and Md Yasin, Ina Salwany (2017) Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia. Journal of Aquatic Animal Health, 29 (4). pp. 208-213. ISSN 0899-7659; ESSN: 1548-8667 http://onlinelibrary.wiley.com/doi/10.1080/08997659.2017.1360411/abstract 10.1080/08997659.2017.1360411 |
| spellingShingle | Rahmatullah, Muhammad Ariff, M. Kahieshesfandiari, M. Mohd Daud, Hassan Saad, Mohd Zamri Mohd Yusoff, Md Sabri Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia |
| title | Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia |
| title_full | Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia |
| title_fullStr | Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia |
| title_full_unstemmed | Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia |
| title_short | Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia |
| title_sort | isolation and pathogenicity of streptococcus iniae in cultured red hybrid tilapia in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/59615/1/Isolation%20and%20pathogenicity%20of%20Streptococcus%20iniae%20in%20cultured%20red%20hybrid%20tilapia%20in%20Malaysia.pdf |
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