Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics
BACKGROUND: Java tea is a well-known herbal infusion prepared from the leaves of Orthosiphon stamineus (OS). The biological properties of tea are in direct correlation with the primary and secondary metabolite composition, which in turn largely depends on the choice of drying method. Herein, the imp...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Society of Chemical Industry
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/59681/1/Identification%20of%20the%20compositional%20changes%20in%20Orthosiphon%20stamineus%20leaves%20triggered%20by%20different%20drying%20techniques%20using%201H%20NMR%20metabolomics.pdf |
| _version_ | 1825932047131082752 |
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| author | Pariyani, Raghunath Ismail, Intan Safinar Ahmad Azam, Amalina Abas, Faridah Shaari, Khozirah |
| author_facet | Pariyani, Raghunath Ismail, Intan Safinar Ahmad Azam, Amalina Abas, Faridah Shaari, Khozirah |
| author_sort | Pariyani, Raghunath |
| collection | UPM |
| description | BACKGROUND: Java tea is a well-known herbal infusion prepared from the leaves of Orthosiphon stamineus (OS). The biological properties of tea are in direct correlation with the primary and secondary metabolite composition, which in turn largely depends on the choice of drying method. Herein, the impact of three commonly used drying methods, i.e. shade, microwave and freeze drying, on the metabolite composition and antioxidant activity of OS leaves was investigated using proton nuclear magnetic resonance (1H NMR) spectroscopy combined with multivariate classification and regression analysis tools.
RESULTS: A total of 31 constituents comprising primary and secondary metabolites belonging to the chemical classes of fatty acids, amino acids, sugars, terpenoids and phenolic compounds were identified. Shade-dried leaves were identified to possess the highest concentrations of bioactive secondary metabolites such as chlorogenic acid, caffeic acid, luteolin, orthosiphol and apigenin, followed by microwave-dried samples. Freeze-dried leaves had higher concentrations of choline, amino acids leucine, alanine and glutamine and sugars such as fructose and α-glucose, but contained the lowest levels of secondary metabolites.
CONCLUSION: Metabolite profiling coupled with multivariate analysis identified shade drying as the best method to prepare OS leaves as Java tea or to include in traditional medicine preparation. |
| first_indexed | 2024-03-06T09:35:59Z |
| format | Article |
| id | upm.eprints-59681 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:35:59Z |
| publishDate | 2017 |
| publisher | Society of Chemical Industry |
| record_format | dspace |
| spelling | upm.eprints-596812018-03-16T01:15:58Z http://psasir.upm.edu.my/id/eprint/59681/ Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics Pariyani, Raghunath Ismail, Intan Safinar Ahmad Azam, Amalina Abas, Faridah Shaari, Khozirah BACKGROUND: Java tea is a well-known herbal infusion prepared from the leaves of Orthosiphon stamineus (OS). The biological properties of tea are in direct correlation with the primary and secondary metabolite composition, which in turn largely depends on the choice of drying method. Herein, the impact of three commonly used drying methods, i.e. shade, microwave and freeze drying, on the metabolite composition and antioxidant activity of OS leaves was investigated using proton nuclear magnetic resonance (1H NMR) spectroscopy combined with multivariate classification and regression analysis tools. RESULTS: A total of 31 constituents comprising primary and secondary metabolites belonging to the chemical classes of fatty acids, amino acids, sugars, terpenoids and phenolic compounds were identified. Shade-dried leaves were identified to possess the highest concentrations of bioactive secondary metabolites such as chlorogenic acid, caffeic acid, luteolin, orthosiphol and apigenin, followed by microwave-dried samples. Freeze-dried leaves had higher concentrations of choline, amino acids leucine, alanine and glutamine and sugars such as fructose and α-glucose, but contained the lowest levels of secondary metabolites. CONCLUSION: Metabolite profiling coupled with multivariate analysis identified shade drying as the best method to prepare OS leaves as Java tea or to include in traditional medicine preparation. Society of Chemical Industry 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59681/1/Identification%20of%20the%20compositional%20changes%20in%20Orthosiphon%20stamineus%20leaves%20triggered%20by%20different%20drying%20techniques%20using%201H%20NMR%20metabolomics.pdf Pariyani, Raghunath and Ismail, Intan Safinar and Ahmad Azam, Amalina and Abas, Faridah and Shaari, Khozirah (2017) Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics. Journal of the Science of Food and Agriculture, 97 (12). pp. 4169-4179. ISSN 0022-5142; ESSN: 1097-0010 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jsfa.8288/abstract 10.1002/jsfa.8288 |
| spellingShingle | Pariyani, Raghunath Ismail, Intan Safinar Ahmad Azam, Amalina Abas, Faridah Shaari, Khozirah Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics |
| title | Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics |
| title_full | Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics |
| title_fullStr | Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics |
| title_full_unstemmed | Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics |
| title_short | Identification of the compositional changes in Orthosiphon stamineus leaves triggered by different drying techniques using 1H NMR metabolomics |
| title_sort | identification of the compositional changes in orthosiphon stamineus leaves triggered by different drying techniques using 1h nmr metabolomics |
| url | http://psasir.upm.edu.my/id/eprint/59681/1/Identification%20of%20the%20compositional%20changes%20in%20Orthosiphon%20stamineus%20leaves%20triggered%20by%20different%20drying%20techniques%20using%201H%20NMR%20metabolomics.pdf |
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