Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro
This paper reports on the extraction, partial characterization and the potential application of crude polysaccharides from defatted coconut residue as a prebiotic. The coconut residue was defatted and extracted to obtain the crude polysaccharides and its physicochemical properties were determined. T...
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| Format: | Article |
| Language: | English |
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Springer
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60573/1/Defatted%20coconut%20residue%20crude%20polysaccharides%20as%20potential%20prebiotics%20study%20of%20their%20effects%20on%20proliferation%20and%20acidifying%20activity%20of%20probiotics%20in%20vitro.pdf |
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| author | Mohd Nor, Nur 'Ain Najwa Abbasiliasi, Sahar Marikkar, Mohammed Nazrim Ariff, Arbakariya Amid, Mehrnoush Lamasudin, Dhilia Udie Abd Manap, Mohd Yazid Mustafa, Shuhaimi |
| author_facet | Mohd Nor, Nur 'Ain Najwa Abbasiliasi, Sahar Marikkar, Mohammed Nazrim Ariff, Arbakariya Amid, Mehrnoush Lamasudin, Dhilia Udie Abd Manap, Mohd Yazid Mustafa, Shuhaimi |
| author_sort | Mohd Nor, Nur 'Ain Najwa |
| collection | UPM |
| description | This paper reports on the extraction, partial characterization and the potential application of crude polysaccharides from defatted coconut residue as a prebiotic. The coconut residue was defatted and extracted to obtain the crude polysaccharides and its physicochemical properties were determined. The crude polysaccharides were assessed for monosaccharide composition, total carbohydrate content, reducing sugar concentration and protein content determination. The functional group and structural elucidation of crude polysaccharides was also done using Fourier transform infrared spectra analysis. The product was then subjected to artificial human gastric juice treatment to determine digestibility. Finally, an in vitro proliferation and acid production by two probiotic bacteria namely Lactobacillus casei Shirota and Lactobacillus bulgaricus were included in this study. It was found that the defatted coconut residue contained ash (0.54%), moisture (55.42%), protein (1.69%), crude fat (17.26%) and carbohydrate (25.73%). The percentage of crude polysaccharides extracted was 0.73 ± 0.04. The two fractions of monosaccharides obtained were glucose and fructose. Total carbohydrate content of DCR was 13.35% (w/v). The quantitative value of the reducing sugars obtained was 20.71%. Protein content in the crude polysaccharides was 0.009% and the peaks which indicated the presence of protein were observed at around 1640 cm−1 (amide I) and 1530 cm−1 (amide II). DCR crude polysaccharides were highly resistant (88%) to hydrolysis when subjected to artificial human gastric juice. The product was found to markedly stimulate two tested probiotics to proliferate and produce organic acids. All the above findings are supportive of the fact that polysaccharides extracted from DCR, an industrial waste, have a vast potential to be exploited as novel prebiotics. |
| first_indexed | 2024-03-06T09:38:34Z |
| format | Article |
| id | upm.eprints-60573 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:38:34Z |
| publishDate | 2017 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-605732018-06-07T08:42:13Z http://psasir.upm.edu.my/id/eprint/60573/ Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro Mohd Nor, Nur 'Ain Najwa Abbasiliasi, Sahar Marikkar, Mohammed Nazrim Ariff, Arbakariya Amid, Mehrnoush Lamasudin, Dhilia Udie Abd Manap, Mohd Yazid Mustafa, Shuhaimi This paper reports on the extraction, partial characterization and the potential application of crude polysaccharides from defatted coconut residue as a prebiotic. The coconut residue was defatted and extracted to obtain the crude polysaccharides and its physicochemical properties were determined. The crude polysaccharides were assessed for monosaccharide composition, total carbohydrate content, reducing sugar concentration and protein content determination. The functional group and structural elucidation of crude polysaccharides was also done using Fourier transform infrared spectra analysis. The product was then subjected to artificial human gastric juice treatment to determine digestibility. Finally, an in vitro proliferation and acid production by two probiotic bacteria namely Lactobacillus casei Shirota and Lactobacillus bulgaricus were included in this study. It was found that the defatted coconut residue contained ash (0.54%), moisture (55.42%), protein (1.69%), crude fat (17.26%) and carbohydrate (25.73%). The percentage of crude polysaccharides extracted was 0.73 ± 0.04. The two fractions of monosaccharides obtained were glucose and fructose. Total carbohydrate content of DCR was 13.35% (w/v). The quantitative value of the reducing sugars obtained was 20.71%. Protein content in the crude polysaccharides was 0.009% and the peaks which indicated the presence of protein were observed at around 1640 cm−1 (amide I) and 1530 cm−1 (amide II). DCR crude polysaccharides were highly resistant (88%) to hydrolysis when subjected to artificial human gastric juice. The product was found to markedly stimulate two tested probiotics to proliferate and produce organic acids. All the above findings are supportive of the fact that polysaccharides extracted from DCR, an industrial waste, have a vast potential to be exploited as novel prebiotics. Springer 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60573/1/Defatted%20coconut%20residue%20crude%20polysaccharides%20as%20potential%20prebiotics%20study%20of%20their%20effects%20on%20proliferation%20and%20acidifying%20activity%20of%20probiotics%20in%20vitro.pdf Mohd Nor, Nur 'Ain Najwa and Abbasiliasi, Sahar and Marikkar, Mohammed Nazrim and Ariff, Arbakariya and Amid, Mehrnoush and Lamasudin, Dhilia Udie and Abd Manap, Mohd Yazid and Mustafa, Shuhaimi (2017) Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro. Journal of Food Science and Technology, 54 (1). pp. 164-173. ISSN 0022-1155; ESSN: 0975-8402 https://link.springer.com/article/10.1007%2Fs13197-016-2448-9 10.1007/s13197-016-2448-9 |
| spellingShingle | Mohd Nor, Nur 'Ain Najwa Abbasiliasi, Sahar Marikkar, Mohammed Nazrim Ariff, Arbakariya Amid, Mehrnoush Lamasudin, Dhilia Udie Abd Manap, Mohd Yazid Mustafa, Shuhaimi Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro |
| title | Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro |
| title_full | Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro |
| title_fullStr | Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro |
| title_full_unstemmed | Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro |
| title_short | Defatted coconut residue crude polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro |
| title_sort | defatted coconut residue crude polysaccharides as potential prebiotics study of their effects on proliferation and acidifying activity of probiotics in vitro |
| url | http://psasir.upm.edu.my/id/eprint/60573/1/Defatted%20coconut%20residue%20crude%20polysaccharides%20as%20potential%20prebiotics%20study%20of%20their%20effects%20on%20proliferation%20and%20acidifying%20activity%20of%20probiotics%20in%20vitro.pdf |
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