Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats
Aims: Antibiotic resistance in Staphylococcus pseudintermedius is increasing gradually towards those antibiotics that are frequently used leading to limited therapeutic options due to multidrug resistance. The objectives of the study were to investigate the antibiotic resistance profiles of S. pseud...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Microbiology
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60604/1/Antibiotic%20resistance%20profiles%20of%20Staphylococcus%20pseudintermedius%20isolated%20from%20dogs%20and%20cats.pdf |
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| author | Mohamed, Mohamed Abdelrahman Abdul Aziz, Saleha Dhaliwal, Gurmeet Kaur Bejo, Siti Khairani Goni, Mohammed Dauda Bitrus, Asinamai Athliamai Muhammad, Ibrahim Jalo |
| author_facet | Mohamed, Mohamed Abdelrahman Abdul Aziz, Saleha Dhaliwal, Gurmeet Kaur Bejo, Siti Khairani Goni, Mohammed Dauda Bitrus, Asinamai Athliamai Muhammad, Ibrahim Jalo |
| author_sort | Mohamed, Mohamed Abdelrahman |
| collection | UPM |
| description | Aims: Antibiotic resistance in Staphylococcus pseudintermedius is increasing gradually towards those antibiotics that are frequently used leading to limited therapeutic options due to multidrug resistance. The objectives of the study were to investigate the antibiotic resistance profiles of S. pseudintermedius isolates from pet and stray dogs and cats in Selangor, Malaysia and to detect the resistance genes (mecA and BlaZ) within the isolates.
Methodology and results: A total of 200 stray and pet dogs and cats were sampled. The samples were cultured onto Mannitol Salt agar and all the presumptive colonies were subcultured, then identified using biochemical tests and confirmed by PCR assay targeting the nuc gene. The isolates were subjected to antibiotic susceptibility test against 12 antibiotics. Twenty three isolates (11.5%) were positive to S. pseudintermedius (stray cats, 11/50; stray dogs, 9/50; pet dogs, 3/50 and pet cats, 0/50). One hundred percent (100%) of the S. pseudintermedius isolates were found to be resistant to penicillin, erythromycin and tetracycline while they showed 100% susceptible to oxacillin, amoxicillinclavulanic acid, gentamicin, chloramphenicol, vancomycin, ciprofloxacin, enrofloxacin, cephalexin and rifampicin. The blaZ gene which codes for β-lactamases production was found in all of the isolates that were resistant to penicillin but not to methicillin.
Conclusion, significance and impact of study: A high number of S. pseudintermedius from dogs and cats developed antibiotic resistance which is a public health concern. |
| first_indexed | 2024-03-06T09:38:40Z |
| format | Article |
| id | upm.eprints-60604 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:38:40Z |
| publishDate | 2017 |
| publisher | Malaysian Society for Microbiology |
| record_format | dspace |
| spelling | upm.eprints-606042018-06-08T00:25:35Z http://psasir.upm.edu.my/id/eprint/60604/ Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats Mohamed, Mohamed Abdelrahman Abdul Aziz, Saleha Dhaliwal, Gurmeet Kaur Bejo, Siti Khairani Goni, Mohammed Dauda Bitrus, Asinamai Athliamai Muhammad, Ibrahim Jalo Aims: Antibiotic resistance in Staphylococcus pseudintermedius is increasing gradually towards those antibiotics that are frequently used leading to limited therapeutic options due to multidrug resistance. The objectives of the study were to investigate the antibiotic resistance profiles of S. pseudintermedius isolates from pet and stray dogs and cats in Selangor, Malaysia and to detect the resistance genes (mecA and BlaZ) within the isolates. Methodology and results: A total of 200 stray and pet dogs and cats were sampled. The samples were cultured onto Mannitol Salt agar and all the presumptive colonies were subcultured, then identified using biochemical tests and confirmed by PCR assay targeting the nuc gene. The isolates were subjected to antibiotic susceptibility test against 12 antibiotics. Twenty three isolates (11.5%) were positive to S. pseudintermedius (stray cats, 11/50; stray dogs, 9/50; pet dogs, 3/50 and pet cats, 0/50). One hundred percent (100%) of the S. pseudintermedius isolates were found to be resistant to penicillin, erythromycin and tetracycline while they showed 100% susceptible to oxacillin, amoxicillinclavulanic acid, gentamicin, chloramphenicol, vancomycin, ciprofloxacin, enrofloxacin, cephalexin and rifampicin. The blaZ gene which codes for β-lactamases production was found in all of the isolates that were resistant to penicillin but not to methicillin. Conclusion, significance and impact of study: A high number of S. pseudintermedius from dogs and cats developed antibiotic resistance which is a public health concern. Malaysian Society for Microbiology 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60604/1/Antibiotic%20resistance%20profiles%20of%20Staphylococcus%20pseudintermedius%20isolated%20from%20dogs%20and%20cats.pdf Mohamed, Mohamed Abdelrahman and Abdul Aziz, Saleha and Dhaliwal, Gurmeet Kaur and Bejo, Siti Khairani and Goni, Mohammed Dauda and Bitrus, Asinamai Athliamai and Muhammad, Ibrahim Jalo (2017) Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats. Malaysian Journal of Microbiology, 13 (3). pp. 180-186. ISSN 1823-8262; ESSN: 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=51 |
| spellingShingle | Mohamed, Mohamed Abdelrahman Abdul Aziz, Saleha Dhaliwal, Gurmeet Kaur Bejo, Siti Khairani Goni, Mohammed Dauda Bitrus, Asinamai Athliamai Muhammad, Ibrahim Jalo Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats |
| title | Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats |
| title_full | Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats |
| title_fullStr | Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats |
| title_full_unstemmed | Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats |
| title_short | Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats |
| title_sort | antibiotic resistance profiles of staphylococcus pseudintermedius isolated from dogs and cats |
| url | http://psasir.upm.edu.my/id/eprint/60604/1/Antibiotic%20resistance%20profiles%20of%20Staphylococcus%20pseudintermedius%20isolated%20from%20dogs%20and%20cats.pdf |
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