A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection
A rapid and simple method for the determination of glyphosate in crude palm oil (CPO) was developed and validated using high performance liquid chromatography with fluorescence detector. Glyphosate was derivatized with 9-fluorenylmethylchloroformate (FMOC-Cl) and then separated using a C18 rever...
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| Format: | Article |
| Language: | English |
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OMICS International
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60701/1/A%20rapid%20liquid%20chromatography%20method%20for%20determination%20of%20gylphosate%20in%20crude%20palm%20oil%20with%20fluorescence%20detection.pdf |
| _version_ | 1825932230379175936 |
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| author | Mohd Salleh, Fariq Fitri Muhamad, Halimah Omar, Dzolkhifli Asib, Norhayu |
| author_facet | Mohd Salleh, Fariq Fitri Muhamad, Halimah Omar, Dzolkhifli Asib, Norhayu |
| author_sort | Mohd Salleh, Fariq Fitri |
| collection | UPM |
| description | A rapid and simple method for the determination of glyphosate in crude palm oil (CPO) was developed and validated using high performance liquid chromatography with fluorescence detector. Glyphosate was derivatized with
9-fluorenylmethylchloroformate (FMOC-Cl) and then separated using a C18 reverse phase column with potassium dihydrogen phosphate and acetonitrile as the mobile phase. A linear correlation was obtained for the concentration of glyphosate from 0.05-1.5 μg mL-1 with a correlation coefficient of 0.9998. The average recovery obtained for glyphosate ranged between 80% and 100% at five fortification levels with the relative standard deviation (RSD) of less than 3% of all cases. The limit of detection and limit of quantification for glyphosate were 0.05 and 0.1 μg/g, respectively. The method will facilitate palm oil trade through quality assurance in terms of glyphosate residues in palm oil products and also to counter any issues related to food safety for palm based products. |
| first_indexed | 2024-03-06T09:38:47Z |
| format | Article |
| id | upm.eprints-60701 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:38:47Z |
| publishDate | 2017 |
| publisher | OMICS International |
| record_format | dspace |
| spelling | upm.eprints-607012018-05-17T03:03:31Z http://psasir.upm.edu.my/id/eprint/60701/ A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection Mohd Salleh, Fariq Fitri Muhamad, Halimah Omar, Dzolkhifli Asib, Norhayu A rapid and simple method for the determination of glyphosate in crude palm oil (CPO) was developed and validated using high performance liquid chromatography with fluorescence detector. Glyphosate was derivatized with 9-fluorenylmethylchloroformate (FMOC-Cl) and then separated using a C18 reverse phase column with potassium dihydrogen phosphate and acetonitrile as the mobile phase. A linear correlation was obtained for the concentration of glyphosate from 0.05-1.5 μg mL-1 with a correlation coefficient of 0.9998. The average recovery obtained for glyphosate ranged between 80% and 100% at five fortification levels with the relative standard deviation (RSD) of less than 3% of all cases. The limit of detection and limit of quantification for glyphosate were 0.05 and 0.1 μg/g, respectively. The method will facilitate palm oil trade through quality assurance in terms of glyphosate residues in palm oil products and also to counter any issues related to food safety for palm based products. OMICS International 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60701/1/A%20rapid%20liquid%20chromatography%20method%20for%20determination%20of%20gylphosate%20in%20crude%20palm%20oil%20with%20fluorescence%20detection.pdf Mohd Salleh, Fariq Fitri and Muhamad, Halimah and Omar, Dzolkhifli and Asib, Norhayu (2017) A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection. Journal of Chromatography & Separation Techiniques, 8 (1). pp. 1-6. ISSN 2157-7064 10.4172/2157-7064.1000346 |
| spellingShingle | Mohd Salleh, Fariq Fitri Muhamad, Halimah Omar, Dzolkhifli Asib, Norhayu A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection |
| title | A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection |
| title_full | A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection |
| title_fullStr | A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection |
| title_full_unstemmed | A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection |
| title_short | A rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection |
| title_sort | rapid liquid chromatography method for determination of gylphosate in crude palm oil with fluorescence detection |
| url | http://psasir.upm.edu.my/id/eprint/60701/1/A%20rapid%20liquid%20chromatography%20method%20for%20determination%20of%20gylphosate%20in%20crude%20palm%20oil%20with%20fluorescence%20detection.pdf |
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