A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus
An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the red...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Open
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60735/1/A%20simple%2C%20portable%2C%20electrochemical%20biosensor%20to%20screen%20shellfish%20for%20vibrio%20parahaemolyticus.pdf |
| _version_ | 1796977357781729280 |
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| author | Nordin, Noordiana Yusof, Nor Azah Abdullah, Jaafar Radu, Son Hushiarian, Roozbeh |
| author_facet | Nordin, Noordiana Yusof, Nor Azah Abdullah, Jaafar Radu, Son Hushiarian, Roozbeh |
| author_sort | Nordin, Noordiana |
| collection | UPM |
| description | An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10−8–2.0 × 10−13 M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool. |
| first_indexed | 2024-03-06T09:38:53Z |
| format | Article |
| id | upm.eprints-60735 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:38:53Z |
| publishDate | 2017 |
| publisher | Springer Open |
| record_format | dspace |
| spelling | upm.eprints-607352019-03-28T04:49:28Z http://psasir.upm.edu.my/id/eprint/60735/ A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus Nordin, Noordiana Yusof, Nor Azah Abdullah, Jaafar Radu, Son Hushiarian, Roozbeh An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10−8–2.0 × 10−13 M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool. Springer Open 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60735/1/A%20simple%2C%20portable%2C%20electrochemical%20biosensor%20to%20screen%20shellfish%20for%20vibrio%20parahaemolyticus.pdf Nordin, Noordiana and Yusof, Nor Azah and Abdullah, Jaafar and Radu, Son and Hushiarian, Roozbeh (2017) A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus. AMB Express, 7 (41). pp. 1-9. ISSN 2191-0855 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311015/pdf/13568_2017_Article_339.pdf 10.1186/s13568-017-0339-8 |
| spellingShingle | Nordin, Noordiana Yusof, Nor Azah Abdullah, Jaafar Radu, Son Hushiarian, Roozbeh A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus |
| title | A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus |
| title_full | A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus |
| title_fullStr | A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus |
| title_full_unstemmed | A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus |
| title_short | A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus |
| title_sort | simple portable electrochemical biosensor to screen shellfish for vibrio parahaemolyticus |
| url | http://psasir.upm.edu.my/id/eprint/60735/1/A%20simple%2C%20portable%2C%20electrochemical%20biosensor%20to%20screen%20shellfish%20for%20vibrio%20parahaemolyticus.pdf |
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