A transmission electron microscopy study of anticoagulant-induced platelet vesiculation
Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to comp...
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Format: | Article |
Language: | English |
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PBJ-Associaco Porto Biomedical
2017
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Online Access: | http://psasir.upm.edu.my/id/eprint/60752/1/A%20transmission%20electron%20microscopy%20study%20of%20anticoagulant-induced%20platelet%20vesiculation.pdf |
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author | Almhanawai, Bahaa Hadi Jaber Khalid, Bahariah Tengku Ibrahim, Tengku Azmi Mohd Tohit, Eusni Rahayu |
author_facet | Almhanawai, Bahaa Hadi Jaber Khalid, Bahariah Tengku Ibrahim, Tengku Azmi Mohd Tohit, Eusni Rahayu |
author_sort | Almhanawai, Bahaa Hadi Jaber |
collection | UPM |
description | Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to compare the direct effects of ethylenediaminetetraacetic acid (EDTA) and sodium citrate anticoagulants on platelet structure and PMP vesiculation using transmission electron microscopy to visualize the morphologic changes in platelets. Micrographs revealed that platelets in the EDTA-anticoagulated tube manifested with significant morphologic changes and induced PMP vesiculation. On the other hand, the sodium citrate-anticoagulated tube showed a normal platelet structure and minor modifications in some cases, with poor indication of PMP vesiculation. In conclusion, EDTA induced platelet activation and PMP vesiculation and represents a major source of artifacts during the pre-analysis steps of PMP vesiculation. |
first_indexed | 2024-03-06T09:38:55Z |
format | Article |
id | upm.eprints-60752 |
institution | Universiti Putra Malaysia |
language | English |
last_indexed | 2024-03-06T09:38:55Z |
publishDate | 2017 |
publisher | PBJ-Associaco Porto Biomedical |
record_format | dspace |
spelling | upm.eprints-607522019-04-29T01:17:05Z http://psasir.upm.edu.my/id/eprint/60752/ A transmission electron microscopy study of anticoagulant-induced platelet vesiculation Almhanawai, Bahaa Hadi Jaber Khalid, Bahariah Tengku Ibrahim, Tengku Azmi Mohd Tohit, Eusni Rahayu Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to compare the direct effects of ethylenediaminetetraacetic acid (EDTA) and sodium citrate anticoagulants on platelet structure and PMP vesiculation using transmission electron microscopy to visualize the morphologic changes in platelets. Micrographs revealed that platelets in the EDTA-anticoagulated tube manifested with significant morphologic changes and induced PMP vesiculation. On the other hand, the sodium citrate-anticoagulated tube showed a normal platelet structure and minor modifications in some cases, with poor indication of PMP vesiculation. In conclusion, EDTA induced platelet activation and PMP vesiculation and represents a major source of artifacts during the pre-analysis steps of PMP vesiculation. PBJ-Associaco Porto Biomedical 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60752/1/A%20transmission%20electron%20microscopy%20study%20of%20anticoagulant-induced%20platelet%20vesiculation.pdf Almhanawai, Bahaa Hadi Jaber and Khalid, Bahariah and Tengku Ibrahim, Tengku Azmi and Mohd Tohit, Eusni Rahayu (2017) A transmission electron microscopy study of anticoagulant-induced platelet vesiculation. Porto Biomedical Journal, 2 (1). 23 - 27. ISSN 2444-8664 https://reader.elsevier.com/reader/sd/pii/S2444866416300964?token=66A46483B82856C546F3C428841960FFF0B96099E7746896F6944458704B7A04F5010626F9684494DCAA4530E9602E61 10.1016/j.pbj.2016.11.002 |
spellingShingle | Almhanawai, Bahaa Hadi Jaber Khalid, Bahariah Tengku Ibrahim, Tengku Azmi Mohd Tohit, Eusni Rahayu A transmission electron microscopy study of anticoagulant-induced platelet vesiculation |
title | A transmission electron microscopy study of anticoagulant-induced platelet vesiculation |
title_full | A transmission electron microscopy study of anticoagulant-induced platelet vesiculation |
title_fullStr | A transmission electron microscopy study of anticoagulant-induced platelet vesiculation |
title_full_unstemmed | A transmission electron microscopy study of anticoagulant-induced platelet vesiculation |
title_short | A transmission electron microscopy study of anticoagulant-induced platelet vesiculation |
title_sort | transmission electron microscopy study of anticoagulant induced platelet vesiculation |
url | http://psasir.upm.edu.my/id/eprint/60752/1/A%20transmission%20electron%20microscopy%20study%20of%20anticoagulant-induced%20platelet%20vesiculation.pdf |
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