Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet
BACKGROUND: Obesity is a major health concern both in developed and developing countries. The use of herbal medicines became the subject of interest for the management of obesity due to its natural origin, cost effectiveness and minimal side effects. The present study aimed at investigating anti-obe...
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| Format: | Article |
| Language: | English |
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BioMed Central
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60891/1/Anti-obesity%20effect%20of%20ethanolic%20extract%20from%20Cosmos%20caudatus%20Kunth%20leaf%20in%20lean%20rats%20fed%20a%20high%20fat%20diet.pdf |
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| author | Abdul Rahman, Hafeedza Sahib, Najla Gooda Saari, Nazamid Abas, Faridah Ismail, Amin Mumtaz, Muhammad Waseem Abdul Hamid, Azizah |
| author_facet | Abdul Rahman, Hafeedza Sahib, Najla Gooda Saari, Nazamid Abas, Faridah Ismail, Amin Mumtaz, Muhammad Waseem Abdul Hamid, Azizah |
| author_sort | Abdul Rahman, Hafeedza |
| collection | UPM |
| description | BACKGROUND: Obesity is a major health concern both in developed and developing countries. The use of herbal medicines became the subject of interest for the management of obesity due to its natural origin, cost effectiveness and minimal side effects. The present study aimed at investigating anti-obesity potential of ethanolic extract from Cosmos caudatus Kunth leaf (EECCL). METHODS: In this study, the rats were randomly divided into six groups i.e., (1) Normal Diet (ND); (2) Normal Diet and 175 mg/kgBW of EECCL (ND + 175 mg/kgBW); (3) Normal Diet and 350 mg/kgBW of EECCL (ND + 350 mg/kgBW); (4) High Fat Diet (HFD); (5) High Fat Diet and 175 mg/kgBW of EECCL (HFD + 175 mg/kgBW); (6) High Fat Diet and 350 mg/kgBW of EECCL (HFD + 350 mg/kgBW). The anti-obesity potential was evaluated through analyses of changes in body weight, visceral fat weight, and blood biochemicals including total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), leptin, insulin, adiponectin, ghrelin and fecal fat content. In addition, metabolite profiling of EECCL was carried out using NMR spectroscopy. RESULTS: Rats receiving EECCL together with HFD showed significant (p < 0.05) reduction in body weight gain compared to rats receiving HFD only. At the end of study, the body weight gain of EECCL treated rats was not significantly (p > 0.05) different with those of ND rats. Other related obesity biomarkers including plasma lipid profiles, insulin, leptin, ghrelin and adiponectin levels also showed significant improvement (p < 0.05). Administration of EECCL caused significant (p < 0.05) increase in fecal fat excretion, which validates the hypothesis of lipase inhibition, an anti-obesity mechanism similar to standard drug of Orlistat. The 1H-NMR spectra of EECCL ascertained the presence of catechin, quercetin, rutin, kaempherol and chlorogenic acid in the extract. CONCLUSION: Conclusively, EECCL showed anti-obesity properties by inhibition of intestinal lipid absorption and modulation of adipocytes markers. |
| first_indexed | 2024-03-06T09:39:11Z |
| format | Article |
| id | upm.eprints-60891 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:39:11Z |
| publishDate | 2017 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | upm.eprints-608912019-04-23T09:04:13Z http://psasir.upm.edu.my/id/eprint/60891/ Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet Abdul Rahman, Hafeedza Sahib, Najla Gooda Saari, Nazamid Abas, Faridah Ismail, Amin Mumtaz, Muhammad Waseem Abdul Hamid, Azizah BACKGROUND: Obesity is a major health concern both in developed and developing countries. The use of herbal medicines became the subject of interest for the management of obesity due to its natural origin, cost effectiveness and minimal side effects. The present study aimed at investigating anti-obesity potential of ethanolic extract from Cosmos caudatus Kunth leaf (EECCL). METHODS: In this study, the rats were randomly divided into six groups i.e., (1) Normal Diet (ND); (2) Normal Diet and 175 mg/kgBW of EECCL (ND + 175 mg/kgBW); (3) Normal Diet and 350 mg/kgBW of EECCL (ND + 350 mg/kgBW); (4) High Fat Diet (HFD); (5) High Fat Diet and 175 mg/kgBW of EECCL (HFD + 175 mg/kgBW); (6) High Fat Diet and 350 mg/kgBW of EECCL (HFD + 350 mg/kgBW). The anti-obesity potential was evaluated through analyses of changes in body weight, visceral fat weight, and blood biochemicals including total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), leptin, insulin, adiponectin, ghrelin and fecal fat content. In addition, metabolite profiling of EECCL was carried out using NMR spectroscopy. RESULTS: Rats receiving EECCL together with HFD showed significant (p < 0.05) reduction in body weight gain compared to rats receiving HFD only. At the end of study, the body weight gain of EECCL treated rats was not significantly (p > 0.05) different with those of ND rats. Other related obesity biomarkers including plasma lipid profiles, insulin, leptin, ghrelin and adiponectin levels also showed significant improvement (p < 0.05). Administration of EECCL caused significant (p < 0.05) increase in fecal fat excretion, which validates the hypothesis of lipase inhibition, an anti-obesity mechanism similar to standard drug of Orlistat. The 1H-NMR spectra of EECCL ascertained the presence of catechin, quercetin, rutin, kaempherol and chlorogenic acid in the extract. CONCLUSION: Conclusively, EECCL showed anti-obesity properties by inhibition of intestinal lipid absorption and modulation of adipocytes markers. BioMed Central 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60891/1/Anti-obesity%20effect%20of%20ethanolic%20extract%20from%20Cosmos%20caudatus%20Kunth%20leaf%20in%20lean%20rats%20fed%20a%20high%20fat%20diet.pdf Abdul Rahman, Hafeedza and Sahib, Najla Gooda and Saari, Nazamid and Abas, Faridah and Ismail, Amin and Mumtaz, Muhammad Waseem and Abdul Hamid, Azizah (2017) Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet. BMC Complementary and Alternative Medicine, 17 (122). pp. 1-17. ISSN 1472-6882 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322639/pdf/12906_2017_Article_1640.pdf 10.1186/s12906-017-1640-4 |
| spellingShingle | Abdul Rahman, Hafeedza Sahib, Najla Gooda Saari, Nazamid Abas, Faridah Ismail, Amin Mumtaz, Muhammad Waseem Abdul Hamid, Azizah Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet |
| title | Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet |
| title_full | Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet |
| title_fullStr | Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet |
| title_full_unstemmed | Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet |
| title_short | Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet |
| title_sort | anti obesity effect of ethanolic extract from cosmos caudatus kunth leaf in lean rats fed a high fat diet |
| url | http://psasir.upm.edu.my/id/eprint/60891/1/Anti-obesity%20effect%20of%20ethanolic%20extract%20from%20Cosmos%20caudatus%20Kunth%20leaf%20in%20lean%20rats%20fed%20a%20high%20fat%20diet.pdf |
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