Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents
Enzyme immobilization via covalent binding provides a strong interaction between enzyme and support material. In this study, the effect of different coupling agents (spacer arms and ligands) in cyclodextrin glucanotransferase (CGTase) immobilization on bleached kenaf microfiber as a support matrix w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61016/1/Bleached%20kenaf%20microfiber%20as%20a%20support%20matrix%20for%20cyclodextrin%20glucanotransferase%20immobilization%20via%20covalent%20binding%20by%20different%20coupling%20agents.pdf |
| _version_ | 1796977398803070976 |
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| author | Ng, Lin Cieh Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli |
| author_facet | Ng, Lin Cieh Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli |
| author_sort | Ng, Lin Cieh |
| collection | UPM |
| description | Enzyme immobilization via covalent binding provides a strong interaction between enzyme and support material. In this study, the effect of different coupling agents (spacer arms and ligands) in cyclodextrin glucanotransferase (CGTase) immobilization on bleached kenaf microfiber as a support matrix was investigated. The immobilized CGTase properties such as storage stability, thermal stability and reusability were evaluated. Immobilized CGTases on microfiber resulted in 0.162–0.24 U/mg-fiber when 55.6 U/mL of CGTase activity was initially added during the immobilization. The highest storage stability (60 °C) was shown by CGTase that was immobilized with ethylenediamine and o-phthalaldehyde, whereby 60% of its activity remained after 15 days. Its high stability was also confirmed by the lowest deactivation constant, kd that was obtained at 25 °C (0.0161 day−1) and 60 °C (0.0361 day−1). The CGTase immobilized using ethylenediamine and glutaraldehyde has shown the best retention of enzyme activity up to 72.72% after 12 cycles of batch reaction. The results indicate that kenaf microfiber has potential to be applied as a support for enzyme immobilization and its enzymatic properties were affected by the coupling agents. |
| first_indexed | 2024-03-06T09:39:29Z |
| format | Article |
| id | upm.eprints-61016 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:39:29Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-610162019-03-20T08:56:19Z http://psasir.upm.edu.my/id/eprint/61016/ Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents Ng, Lin Cieh Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli Enzyme immobilization via covalent binding provides a strong interaction between enzyme and support material. In this study, the effect of different coupling agents (spacer arms and ligands) in cyclodextrin glucanotransferase (CGTase) immobilization on bleached kenaf microfiber as a support matrix was investigated. The immobilized CGTase properties such as storage stability, thermal stability and reusability were evaluated. Immobilized CGTases on microfiber resulted in 0.162–0.24 U/mg-fiber when 55.6 U/mL of CGTase activity was initially added during the immobilization. The highest storage stability (60 °C) was shown by CGTase that was immobilized with ethylenediamine and o-phthalaldehyde, whereby 60% of its activity remained after 15 days. Its high stability was also confirmed by the lowest deactivation constant, kd that was obtained at 25 °C (0.0161 day−1) and 60 °C (0.0361 day−1). The CGTase immobilized using ethylenediamine and glutaraldehyde has shown the best retention of enzyme activity up to 72.72% after 12 cycles of batch reaction. The results indicate that kenaf microfiber has potential to be applied as a support for enzyme immobilization and its enzymatic properties were affected by the coupling agents. Elsevier 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61016/1/Bleached%20kenaf%20microfiber%20as%20a%20support%20matrix%20for%20cyclodextrin%20glucanotransferase%20immobilization%20via%20covalent%20binding%20by%20different%20coupling%20agents.pdf Ng, Lin Cieh and Sulaiman, Safwan and Mokhtar, Mohd Noriznan and Naim, Mohd Nazli (2017) Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents. Process Biochemistry (56). 81 - 89. ISSN 1359-5113; ESSN: 1873-3298 10.1016/j.procbio.2017.02.025 |
| spellingShingle | Ng, Lin Cieh Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents |
| title | Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents |
| title_full | Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents |
| title_fullStr | Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents |
| title_full_unstemmed | Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents |
| title_short | Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents |
| title_sort | bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents |
| url | http://psasir.upm.edu.my/id/eprint/61016/1/Bleached%20kenaf%20microfiber%20as%20a%20support%20matrix%20for%20cyclodextrin%20glucanotransferase%20immobilization%20via%20covalent%20binding%20by%20different%20coupling%20agents.pdf |
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