Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy

It is known from our previous studies that kiwifruits, which are used in common human diet, have preventive properties of coronary artery disease. This study describes a combination of 1H NMR spectroscopy, multivariate data analyses and fluorescence measurements in differentiating of some kiwifruit...

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Main Authors: Abdul Hamid, Nur Ashikin, Mediani, Ahmed, Maulidiani, M., Abas, Faridah, Yong, Seo Park, Leontowicz, Hanna, Leontowicz, Maria, Namiesnik, Jacek, Gorinstein, S.
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61098/1/Characterization%20of%20metabolites%20in%20different%20kiwifruit%20varieties%20by%20NMR%20and%20fluorescence%20spectroscopy.pdf
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author Abdul Hamid, Nur Ashikin
Mediani, Ahmed
Maulidiani, M.
Abas, Faridah
Yong, Seo Park
Leontowicz, Hanna
Leontowicz, Maria
Namiesnik, Jacek
Gorinstein, S.
author_facet Abdul Hamid, Nur Ashikin
Mediani, Ahmed
Maulidiani, M.
Abas, Faridah
Yong, Seo Park
Leontowicz, Hanna
Leontowicz, Maria
Namiesnik, Jacek
Gorinstein, S.
author_sort Abdul Hamid, Nur Ashikin
collection UPM
description It is known from our previous studies that kiwifruits, which are used in common human diet, have preventive properties of coronary artery disease. This study describes a combination of 1H NMR spectroscopy, multivariate data analyses and fluorescence measurements in differentiating of some kiwifruit varieties, their quenching and antioxidant properties. A total of 41 metabolites were identified by comparing with literature data Chenomx database and 2D NMR. The binding properties of the extracted polyphenols against HSA showed higher reactivity of studied two cultivars in comparison with the common Hayward. The results showed that the fluorescence of HSA was quenched by Bidan as much as twice than by other fruits. The correlation between the binding properties of polyphenols in the investigated fruits, their relative quantification and suggested metabolic pathway was established. These results can provide possible application of fruit extracts in pharmaceutical industry.
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spelling upm.eprints-610982018-09-20T03:45:38Z http://psasir.upm.edu.my/id/eprint/61098/ Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy Abdul Hamid, Nur Ashikin Mediani, Ahmed Maulidiani, M. Abas, Faridah Yong, Seo Park Leontowicz, Hanna Leontowicz, Maria Namiesnik, Jacek Gorinstein, S. It is known from our previous studies that kiwifruits, which are used in common human diet, have preventive properties of coronary artery disease. This study describes a combination of 1H NMR spectroscopy, multivariate data analyses and fluorescence measurements in differentiating of some kiwifruit varieties, their quenching and antioxidant properties. A total of 41 metabolites were identified by comparing with literature data Chenomx database and 2D NMR. The binding properties of the extracted polyphenols against HSA showed higher reactivity of studied two cultivars in comparison with the common Hayward. The results showed that the fluorescence of HSA was quenched by Bidan as much as twice than by other fruits. The correlation between the binding properties of polyphenols in the investigated fruits, their relative quantification and suggested metabolic pathway was established. These results can provide possible application of fruit extracts in pharmaceutical industry. Elsevier 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61098/1/Characterization%20of%20metabolites%20in%20different%20kiwifruit%20varieties%20by%20NMR%20and%20fluorescence%20spectroscopy.pdf Abdul Hamid, Nur Ashikin and Mediani, Ahmed and Maulidiani, M. and Abas, Faridah and Yong, Seo Park and Leontowicz, Hanna and Leontowicz, Maria and Namiesnik, Jacek and Gorinstein, S. (2017) Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy. Journal of Pharmaceutical and Biomedical Analysis, 138. pp. 80-91. ISSN 0731-7085; ESSN: 1873-264X https://reader.elsevier.com/reader/sd/pii/S0731708516309293?token=942108EA904CF04C2787486631F7001E09001FA36C69823563F75104B28525C15B234C1890DEDCBEEFB22AFB33835A06 10.1016/j.jpba.2017.01.046
spellingShingle Abdul Hamid, Nur Ashikin
Mediani, Ahmed
Maulidiani, M.
Abas, Faridah
Yong, Seo Park
Leontowicz, Hanna
Leontowicz, Maria
Namiesnik, Jacek
Gorinstein, S.
Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy
title Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy
title_full Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy
title_fullStr Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy
title_full_unstemmed Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy
title_short Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy
title_sort characterization of metabolites in different kiwifruit varieties by nmr and fluorescence spectroscopy
url http://psasir.upm.edu.my/id/eprint/61098/1/Characterization%20of%20metabolites%20in%20different%20kiwifruit%20varieties%20by%20NMR%20and%20fluorescence%20spectroscopy.pdf
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