Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure
In this study, we developed a mouse model and characterized the effects of intranasal inoculation of virulent Brucella melitensis strain 16M and its lipopolysaccharide (LPS). The effects of the exposure were compared with respective control groups. Both Brucella melitensis-infected and LPS-infected...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61166/1/LIPO.pdf |
| _version_ | 1796977422759886848 |
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| author | Osman, Abdinasir Yusuf Saharee, Abdul Aziz Abdullah, Faez Firdaus Jesse Abdul Kadir, Arifah |
| author_facet | Osman, Abdinasir Yusuf Saharee, Abdul Aziz Abdullah, Faez Firdaus Jesse Abdul Kadir, Arifah |
| author_sort | Osman, Abdinasir Yusuf |
| collection | UPM |
| description | In this study, we developed a mouse model and characterized the effects of intranasal inoculation of virulent Brucella melitensis strain 16M and its lipopolysaccharide (LPS). The effects of the exposure were compared with respective control groups. Both Brucella melitensis-infected and LPS-infected groups showed no significant clinical presentation with minor relevance in the mortality associated with the infection. In Brucella melitensis-infected group, significant histopathological changes in comparison to the LPS infected group with increase bacterial burden in the lungs, reproductive and reticuloendothelial organs were observed. However, both infected groups showed elevated levels of pro-inflammatory cytokine expression (IL-1β and IL6) and antibody production (IgM an IgG) as early as 3 days post-infection with predominance in LPS infected group. In contrast, low levels of sex related hormonal changes was recorded in both infected groups throughout the experimental period. This is the first detailed investigation comparing the infection progression and host responses in relation to the immunopathophysiological aspects in mouse model after intranasal inoculation with B. melitensis and its lipopolysaccharide. The study revealed a significant difference between infected and control groups with overlap in clinical, pathological, and immunological responses as well as sex related hormonal changes resulting from the infections. |
| first_indexed | 2024-03-06T09:39:54Z |
| format | Article |
| id | upm.eprints-61166 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:39:54Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-611662021-07-17T22:34:12Z http://psasir.upm.edu.my/id/eprint/61166/ Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure Osman, Abdinasir Yusuf Saharee, Abdul Aziz Abdullah, Faez Firdaus Jesse Abdul Kadir, Arifah In this study, we developed a mouse model and characterized the effects of intranasal inoculation of virulent Brucella melitensis strain 16M and its lipopolysaccharide (LPS). The effects of the exposure were compared with respective control groups. Both Brucella melitensis-infected and LPS-infected groups showed no significant clinical presentation with minor relevance in the mortality associated with the infection. In Brucella melitensis-infected group, significant histopathological changes in comparison to the LPS infected group with increase bacterial burden in the lungs, reproductive and reticuloendothelial organs were observed. However, both infected groups showed elevated levels of pro-inflammatory cytokine expression (IL-1β and IL6) and antibody production (IgM an IgG) as early as 3 days post-infection with predominance in LPS infected group. In contrast, low levels of sex related hormonal changes was recorded in both infected groups throughout the experimental period. This is the first detailed investigation comparing the infection progression and host responses in relation to the immunopathophysiological aspects in mouse model after intranasal inoculation with B. melitensis and its lipopolysaccharide. The study revealed a significant difference between infected and control groups with overlap in clinical, pathological, and immunological responses as well as sex related hormonal changes resulting from the infections. Elsevier 2017-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61166/1/LIPO.pdf Osman, Abdinasir Yusuf and Saharee, Abdul Aziz and Abdullah, Faez Firdaus Jesse and Abdul Kadir, Arifah (2017) Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure. Microbial Pathogenesis, 110. 365 - 374. ISSN 0882-4010; ESSN: 1096-1208 https://www.sciencedirect.com/science/article/pii/S0882401016307069 10.1016/j.micpath.2017.07.014 |
| spellingShingle | Osman, Abdinasir Yusuf Saharee, Abdul Aziz Abdullah, Faez Firdaus Jesse Abdul Kadir, Arifah Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure |
| title | Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure |
| title_full | Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure |
| title_fullStr | Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure |
| title_full_unstemmed | Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure |
| title_short | Comparative study of immunopathophysiological responses induced by B. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure |
| title_sort | comparative study of immunopathophysiological responses induced by b melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure |
| url | http://psasir.upm.edu.my/id/eprint/61166/1/LIPO.pdf |
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