Determination of Poly-β-Hydroxyalkanoate in peat
Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material that has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61429/1/Determination%20of%20Poly-%CE%B2-hydroxyalkanoate%20in%20peat.pdf |
| _version_ | 1825932335845998592 |
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| author | Dowrick, David J. Md Yusoff, Fatimah Gough, Rachel Freeman, Christopher |
| author_facet | Dowrick, David J. Md Yusoff, Fatimah Gough, Rachel Freeman, Christopher |
| author_sort | Dowrick, David J. |
| collection | UPM |
| description | Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material that has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change. |
| first_indexed | 2024-03-06T09:40:27Z |
| format | Article |
| id | upm.eprints-61429 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:40:27Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-614292018-05-23T00:36:05Z http://psasir.upm.edu.my/id/eprint/61429/ Determination of Poly-β-Hydroxyalkanoate in peat Dowrick, David J. Md Yusoff, Fatimah Gough, Rachel Freeman, Christopher Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material that has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change. Taylor & Francis 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61429/1/Determination%20of%20Poly-%CE%B2-hydroxyalkanoate%20in%20peat.pdf Dowrick, David J. and Md Yusoff, Fatimah and Gough, Rachel and Freeman, Christopher (2017) Determination of Poly-β-Hydroxyalkanoate in peat. Communications in Soil Science and Plant Analysis, 48 (5). 576 - 580. ISSN 0010-3624; ESSN: 1532-2416 https://www.tandfonline.com/doi/abs/10.1080/00103624.2017.1282506 10.1080/00103624.2017.1282506 |
| spellingShingle | Dowrick, David J. Md Yusoff, Fatimah Gough, Rachel Freeman, Christopher Determination of Poly-β-Hydroxyalkanoate in peat |
| title | Determination of Poly-β-Hydroxyalkanoate in peat |
| title_full | Determination of Poly-β-Hydroxyalkanoate in peat |
| title_fullStr | Determination of Poly-β-Hydroxyalkanoate in peat |
| title_full_unstemmed | Determination of Poly-β-Hydroxyalkanoate in peat |
| title_short | Determination of Poly-β-Hydroxyalkanoate in peat |
| title_sort | determination of poly β hydroxyalkanoate in peat |
| url | http://psasir.upm.edu.my/id/eprint/61429/1/Determination%20of%20Poly-%CE%B2-hydroxyalkanoate%20in%20peat.pdf |
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