Efects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology
Background: Clinacanthus nutans Lindau is a well-known plant, native to tropical Asian countries. Reports on this plant that is rich in phenolic compounds have focused on its therapeutic anti-inflammatory, anti-herpes simplex, antioxidant, and anti-cancer characteristics. In this paper, the influenc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Chemistry Central
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61675/1/Efects%20of%C2%A0temperature%2C%20time%2C%20and%C2%A0solvent.pdf |
| _version_ | 1796977500109144064 |
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| author | Che Sulaiman, Intan Soraya Basri, Mahiran Masoumi, Hamid Reza Fard Chee, Wei Jian Ashari, Siti Efiza Ismail, Maznah |
| author_facet | Che Sulaiman, Intan Soraya Basri, Mahiran Masoumi, Hamid Reza Fard Chee, Wei Jian Ashari, Siti Efiza Ismail, Maznah |
| author_sort | Che Sulaiman, Intan Soraya |
| collection | UPM |
| description | Background: Clinacanthus nutans Lindau is a well-known plant, native to tropical Asian countries. Reports on this plant that is rich in phenolic compounds have focused on its therapeutic anti-inflammatory, anti-herpes simplex, antioxidant, and anti-cancer characteristics. In this paper, the influence of the extraction parameters—temperatures (60–80 °C), times (80–120 min), and solvent ratios (70:30–90:10) of water:ethanol were investigated using response surface methodology in order to determine the optimum extraction conditions that could produce maximum extraction yields of the phenolic compounds and the highest anti-radical activity of the C. nutans extract. Results: The optimum conditions suggested by the predicted model were: an extraction temperature of 60 °C, an extraction time of 120 min and a water:ethanol solvent ratio of 90:10 v/v%. The residual standard error of 0.2% indicated that there was no significant difference between the actual and predicted values and it proved that the models were adequate to predict the relevant responses. All the independent variables had a significant effect (p < 0.05) on all the responses which indicated that all extraction parameters employed in this study were important in the optimization process. The R2 values for three responses, extraction yields, DPPH radical scavenging activity and TPC were 0.9999, 0.9999 and 0.9983 respectively, suggesting that the quadratic polynomial models developed were satisfactorily accurate to be used in analyzing the interactions of the parameters (response and independent variables). Conclusion:
This study could be useful in the development of cosmeceutical products containing extracts of C. nutans. |
| first_indexed | 2024-03-06T09:41:02Z |
| format | Article |
| id | upm.eprints-61675 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:41:02Z |
| publishDate | 2017 |
| publisher | Chemistry Central |
| record_format | dspace |
| spelling | upm.eprints-616752018-10-31T00:59:00Z http://psasir.upm.edu.my/id/eprint/61675/ Efects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology Che Sulaiman, Intan Soraya Basri, Mahiran Masoumi, Hamid Reza Fard Chee, Wei Jian Ashari, Siti Efiza Ismail, Maznah Background: Clinacanthus nutans Lindau is a well-known plant, native to tropical Asian countries. Reports on this plant that is rich in phenolic compounds have focused on its therapeutic anti-inflammatory, anti-herpes simplex, antioxidant, and anti-cancer characteristics. In this paper, the influence of the extraction parameters—temperatures (60–80 °C), times (80–120 min), and solvent ratios (70:30–90:10) of water:ethanol were investigated using response surface methodology in order to determine the optimum extraction conditions that could produce maximum extraction yields of the phenolic compounds and the highest anti-radical activity of the C. nutans extract. Results: The optimum conditions suggested by the predicted model were: an extraction temperature of 60 °C, an extraction time of 120 min and a water:ethanol solvent ratio of 90:10 v/v%. The residual standard error of 0.2% indicated that there was no significant difference between the actual and predicted values and it proved that the models were adequate to predict the relevant responses. All the independent variables had a significant effect (p < 0.05) on all the responses which indicated that all extraction parameters employed in this study were important in the optimization process. The R2 values for three responses, extraction yields, DPPH radical scavenging activity and TPC were 0.9999, 0.9999 and 0.9983 respectively, suggesting that the quadratic polynomial models developed were satisfactorily accurate to be used in analyzing the interactions of the parameters (response and independent variables). Conclusion: This study could be useful in the development of cosmeceutical products containing extracts of C. nutans. Chemistry Central 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61675/1/Efects%20of%C2%A0temperature%2C%20time%2C%20and%C2%A0solvent.pdf Che Sulaiman, Intan Soraya and Basri, Mahiran and Masoumi, Hamid Reza Fard and Chee, Wei Jian and Ashari, Siti Efiza and Ismail, Maznah (2017) Efects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology. Chemistry Central Journal, 11. pp. 1-11. ISSN 1752-153X https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471285/ 10.1186/s13065-017-0285-1 |
| spellingShingle | Che Sulaiman, Intan Soraya Basri, Mahiran Masoumi, Hamid Reza Fard Chee, Wei Jian Ashari, Siti Efiza Ismail, Maznah Efects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology |
| title | Efects of temperature, time, and solvent
ratio on the extraction of phenolic compounds
and the anti-radical activity of Clinacanthus
nutans Lindau leaves by response surface
methodology |
| title_full | Efects of temperature, time, and solvent
ratio on the extraction of phenolic compounds
and the anti-radical activity of Clinacanthus
nutans Lindau leaves by response surface
methodology |
| title_fullStr | Efects of temperature, time, and solvent
ratio on the extraction of phenolic compounds
and the anti-radical activity of Clinacanthus
nutans Lindau leaves by response surface
methodology |
| title_full_unstemmed | Efects of temperature, time, and solvent
ratio on the extraction of phenolic compounds
and the anti-radical activity of Clinacanthus
nutans Lindau leaves by response surface
methodology |
| title_short | Efects of temperature, time, and solvent
ratio on the extraction of phenolic compounds
and the anti-radical activity of Clinacanthus
nutans Lindau leaves by response surface
methodology |
| title_sort | efects of temperature time and solvent ratio on the extraction of phenolic compounds and the anti radical activity of clinacanthus nutans lindau leaves by response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/61675/1/Efects%20of%C2%A0temperature%2C%20time%2C%20and%C2%A0solvent.pdf |
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