Expression of human cytokine genes associated with chronic Hepatitis B disease progression
Background: Hepatitis viruses are non-cytopathic viruses that lead to the infection and pathogenesis of liver diseases as a result of immunologically mediated event. Objective: To investigate the expression of human inflammatory cytokines in chronic hepatitis B patients according to the severity of...
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| Format: | Article |
| Language: | English |
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Iranian Society for Immunology & Allergy
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61824/1/Expression%20of%20human%20cytokine%20genes%20associated%20with%20chronic%20Hepatitis%20B%20disease%20progression.pdf |
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| author | Hudu, Shuaibu Abdullahi Niazlin, Mohd Taib Amin Nordin, Syafinaz Saeed, Mohammed Ibrahim Tan, Soek Siam Sekawi, Zamberi |
| author_facet | Hudu, Shuaibu Abdullahi Niazlin, Mohd Taib Amin Nordin, Syafinaz Saeed, Mohammed Ibrahim Tan, Soek Siam Sekawi, Zamberi |
| author_sort | Hudu, Shuaibu Abdullahi |
| collection | UPM |
| description | Background: Hepatitis viruses are non-cytopathic viruses that lead to the infection and pathogenesis of liver diseases as a result of immunologically mediated event. Objective: To investigate the expression of human inflammatory cytokines in chronic hepatitis B patients according to the severity of the infection. Methods: We recruited a total of 120 patients, 40 of whom from cirrhotic, 40 non-cirrhotic, and 40 acute flare chronic hepatitis B and 40 healthy controls. For all groups total cellular RNA was extracted from whole blood samples, genomic DNA was eliminated, and cDNA was synthesized using the RT2 first strand kit, as instructed by the manufacturer. The real-time profiler PCR array was performed on an a master cycler ep realplex and the data were analyzed using an online data analysis software. Results: Non-cirrhotic chronic hepatitis B patients were found to significantly upregulate interleukin 10 receptors that regulate the balance between T helpers 1 and 2. On the other hand, patients with cirrhosis were found to have significant upregulated interleukin 3 gene expression. Conclusion: Our finding suggests that upregulation of anti-inflammatory and downregulation of pro-inflammatory cytokines may play a roles in the progression of non-cirrhotic chronic hepatitis B patients to cirrhotic and acute flare. However, a multi-center study with a larger sample size is needed to confirm our findings. |
| first_indexed | 2024-03-06T09:41:23Z |
| format | Article |
| id | upm.eprints-61824 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:41:23Z |
| publishDate | 2017 |
| publisher | Iranian Society for Immunology & Allergy |
| record_format | dspace |
| spelling | upm.eprints-618242019-01-30T04:56:08Z http://psasir.upm.edu.my/id/eprint/61824/ Expression of human cytokine genes associated with chronic Hepatitis B disease progression Hudu, Shuaibu Abdullahi Niazlin, Mohd Taib Amin Nordin, Syafinaz Saeed, Mohammed Ibrahim Tan, Soek Siam Sekawi, Zamberi Background: Hepatitis viruses are non-cytopathic viruses that lead to the infection and pathogenesis of liver diseases as a result of immunologically mediated event. Objective: To investigate the expression of human inflammatory cytokines in chronic hepatitis B patients according to the severity of the infection. Methods: We recruited a total of 120 patients, 40 of whom from cirrhotic, 40 non-cirrhotic, and 40 acute flare chronic hepatitis B and 40 healthy controls. For all groups total cellular RNA was extracted from whole blood samples, genomic DNA was eliminated, and cDNA was synthesized using the RT2 first strand kit, as instructed by the manufacturer. The real-time profiler PCR array was performed on an a master cycler ep realplex and the data were analyzed using an online data analysis software. Results: Non-cirrhotic chronic hepatitis B patients were found to significantly upregulate interleukin 10 receptors that regulate the balance between T helpers 1 and 2. On the other hand, patients with cirrhosis were found to have significant upregulated interleukin 3 gene expression. Conclusion: Our finding suggests that upregulation of anti-inflammatory and downregulation of pro-inflammatory cytokines may play a roles in the progression of non-cirrhotic chronic hepatitis B patients to cirrhotic and acute flare. However, a multi-center study with a larger sample size is needed to confirm our findings. Iranian Society for Immunology & Allergy 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61824/1/Expression%20of%20human%20cytokine%20genes%20associated%20with%20chronic%20Hepatitis%20B%20disease%20progression.pdf Hudu, Shuaibu Abdullahi and Niazlin, Mohd Taib and Amin Nordin, Syafinaz and Saeed, Mohammed Ibrahim and Tan, Soek Siam and Sekawi, Zamberi (2017) Expression of human cytokine genes associated with chronic Hepatitis B disease progression. Iranian Journal of Immunology, 14 (4). 281 - 292. ISSN 1735-1383; ESSN: 1735-367X http://iji.sums.ac.ir/?sid=Entrez:PubMed&id=pmid:29276181&key=2017.14.4.281 |
| spellingShingle | Hudu, Shuaibu Abdullahi Niazlin, Mohd Taib Amin Nordin, Syafinaz Saeed, Mohammed Ibrahim Tan, Soek Siam Sekawi, Zamberi Expression of human cytokine genes associated with chronic Hepatitis B disease progression |
| title | Expression of human cytokine genes associated with chronic Hepatitis B disease progression |
| title_full | Expression of human cytokine genes associated with chronic Hepatitis B disease progression |
| title_fullStr | Expression of human cytokine genes associated with chronic Hepatitis B disease progression |
| title_full_unstemmed | Expression of human cytokine genes associated with chronic Hepatitis B disease progression |
| title_short | Expression of human cytokine genes associated with chronic Hepatitis B disease progression |
| title_sort | expression of human cytokine genes associated with chronic hepatitis b disease progression |
| url | http://psasir.upm.edu.my/id/eprint/61824/1/Expression%20of%20human%20cytokine%20genes%20associated%20with%20chronic%20Hepatitis%20B%20disease%20progression.pdf |
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